Bacteriostatic activity expressed by MIC values of S. aureus 209P was compared with MIC values for B. subtilis 6633 on 26 propolis extracts. Extracts with different MIC values ranging from a 0.060 to 0.600 mg/ml basis were tested. The determined MIC values for both species of bacteria were found to be identical in 19 samples of propolis extracts. Small differences in MIC values were observed in 7 samples. The differences did not exceed 0.010 mg/ml in 3 and 0.020 mg/ml of medium in 4 samples. The results showed that for the determination of bacteriostatic activity of propolis extracts the application of B. subtilis 6633 yielded an identical value to S. aureus 209P.