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2007 | 58 | 4 |

Tytuł artykułu

Beta-carotene and arachidonic acid induced DNA methylation and the regulation of pro-chemotactic activity of endothelial cells and its progenitors

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
DNA methylation is one of the important mechanisms regulating gene expression. Since beta-carotene (BC) was shown to have pro-chemotactic activity and stimulates expression of pro-angiogenic genes, this study was undertaken to define the possible changes in DNA methylation in endothelial cell and its progenitors in the presence of BC. The culture medium for human umbilical vein endothelial cells (HUVEC) and endothelial progenitor cells (EPC) was supplemented with BC (1 - 10 µM) with the presence of arachidonic acid (AA) (3 µM). Global DNA methylation tended to be lower in both endothelial cell lines, after incubation with BC and AA. HUVEC incubated with AA demonstrated the lowest DNA methylation. The decrease of DNA methylation in EPC, induced by BC, was concentration - dependent. The microarray study revealed, that the angiogenesis and homing – related genes were mostly influenced by BC and AA in investigated cells. Our results indicate that BC and AA-induced DNA hypomethylation in EPC and HUVEC, might be a mechanism which may alter gene expression in endothelial cells what in certain conditions may be connected with the suggested pro-malignant effect of this compounds.

Wydawca

-

Rocznik

Tom

58

Numer

4

Opis fizyczny

p.757-766,fig.,ref.

Twórcy

autor
  • Jagiellonian University, Kopernika 15a, 30-504 Krakow, Poland
autor
autor

Bibliografia

Typ dokumentu

Bibliografia

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Identyfikator YADDA

bwmeta1.element.agro-article-2790ea2c-ecec-4434-a7a5-64c5c3a4f055
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