Beta-carotene and arachidonic acid induced DNA methylation and the regulation of pro-chemotactic activity of endothelial cells and its progenitors

• Autorzy: Kiec-Wilk B. , Polus A. , Mikolajczyk M. , Mathers J.C.
• Języki publikacji: EN

Abstrakty

EN

DNA methylation is one of the important mechanisms regulating gene expression. Since beta-carotene (BC) was shown to have pro-chemotactic activity and stimulates expression of pro-angiogenic genes, this study was undertaken to define the possible changes in DNA methylation in endothelial cell and its progenitors in the presence of BC. The culture medium for human umbilical vein endothelial cells (HUVEC) and endothelial progenitor cells (EPC) was supplemented with BC (1 - 10 µM) with the presence of arachidonic acid (AA) (3 µM). Global DNA methylation tended to be lower in both endothelial cell lines, after incubation with BC and AA. HUVEC incubated with AA demonstrated the lowest DNA methylation. The decrease of DNA methylation in EPC, induced by BC, was concentration - dependent. The microarray study revealed, that the angiogenesis and homing – related genes were mostly influenced by BC and AA in investigated cells. Our results indicate that BC and AA-induced DNA hypomethylation in EPC and HUVEC, might be a mechanism which may alter gene expression in endothelial cells what in certain conditions may be connected with the suggested pro-malignant effect of this compounds.

Słowa kluczowe

EN

pro-chemotactic activity; beta-carotene; arachidonic acid; human umbilical vein endothelial cell; gene expression; endothelial cell; angiogenesis; progenitor; endothelial progenitor cell; DNA methylation

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 4
• Opis fizyczny: p.757-766,fig.,ref.

Twórcy

autor

Kiec-Wilk B.

• Jagiellonian University, Kopernika 15a, 30-504 Krakow, Poland

autor

Polus A.

autor

Mikolajczyk M.

autor

Mathers J.C.