The application of flow cytometric assay for evaluation of phagocytosis of neutrophils

• Autorzy: Zielinska M. , Fenrych W.
• Języki publikacji: EN

Abstrakty

EN

Phagocytosis and the release of oxidative products generated by the respira­tory burst have been studied in vitro under the influence of non-steroidal anti-inflammatory drugs: naproxen and ibuprofen, using.phagocytes of periph­eral blood from healthy human donors. Phagocytosis wis monitored by flow cytometry in order to investigate the uptake of propvdium iodide-labelled bacteria (Staphylococcus aureus) by polymorphonuclear leucocytes. In addi­tion, the phagocytic capacity and percentage of killed bacteria was measured in isolated neutrophils using the Pantazis & Kniker method. It was found that naproxen and ibuprofen affect the phagocytic function and hydrogen peroxide production in the examined granulocytes.

Słowa kluczowe

EN

granulocyte; neutrophil function; production; antiinflammatory drug; 2',7'-dichlorofluorescein diacetate; phagocytosis; hydrogen peroxide; Staphylococcus aureus; bacteria; flow cytometry; phagocytic function

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 1
• Opis fizyczny: p.121-125,fig.

Twórcy

autor

Zielinska M.

• Karol Marcinkowski University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland

autor

Fenrych W.

Bibliografia

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