Micropropagation of Senecio macrophyllus M.Bieb.

• Autorzy: Trejgell A , Michalska M. , tretyn A.
• Języki publikacji: EN

Abstrakty

EN

This is the first protocol for in vitro micropropagation of Senecio macrophyllus. Shoot tips and fragments of the cotyledon, hypocotyls and roots were isolated from 10-day-old sterile seedlings. The morphological response was tested on MS medium supplemented with different types of cytokinins: BA (2.2 μM, 4.4 μM or 13.3 μM), KN (4.7 μM or 13.9 μM) and ZEA (4.6 μM or 13,7 μM) in combination with 0.54 μM NAA or 0.27 μM NAA (with 2.2 μM BA only), but only shoot tips were capable of shoot organogenesis. Shoot proliferation was highest for explants cultured on MS medium supplemented with 4.4 μM BA in combination with 0.54 μM NAA. The shoots formed were then multiplied on the same medium. Rooting was achieved on full- and half-strength MS medium without auxin, but shoots cultured on medium BA-supplemented began inducing roots a week later than shoots obtained on media with other types of cytokinins. Well-rooted plantlets were transferred to ex vitro conditions. The survival rate of rooted plants was 100% for plants cultured in a mixture of vermiculite and sand, and 92% for those planted in soil after 4 weeks of acclimatization. In the first year the plants grew intensively under field conditions and were able to develop a leaf rosette. In the second year the plants were able to flower and produce viable seeds.

Słowa kluczowe

EN

Compositae; Senecio macrophyllus; shoot tip; cotyledon; micropropagation; flowering plantlet; benzylaminopurine

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2010
• Tom: 52
• Numer: 1
• Opis fizyczny: p.67-72,fig.,ref.

Twórcy

autor

Trejgell A

• Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

autor

Michalska M.

autor

tretyn A.

Bibliografia

• BASKARAN P, and JAYABALAN N. 2005. An efficient micropropagation system for Eclipa alba – a valuable medicinalherb. In Vitro Cellular and Developmental Biology –Plant 41: 532–539.
• BIRMETA G, and WELANDER M. 2004. Efficient micropropagation of Ensete ventricosum applying meristem wounding:a three-step protocol. Plant Cell Reports 23:277–283.
• BRAMWELL B. 1990. The role of in vitro cultivation in the conservation of endangered species. In: Hernandez BermejoJE, Clemente M, Heywood V [eds.], ConservationTechniques in Botanic Garden, 3–15. Koeltz ScientificBooks, Koenigstein.
• CHAUDHURI KN, GHOSH B, and JHA S. 2004. The root: a potential new source of competent cells for high-frequencyregeneration in Tylophora indica. Plant Cell Reports 22:731–740.
• CUENCA S, AMO-MARCO JB, and PARRA R. 1999. Micropropagation from inflorescence stems of the Spanish endemicplant Centaurea paui Loscos ex Willk. (Compositae).Plant Cell Reports 18: 674–679.
• CZARNECKA B. 1995. Biology and ecology of the isolated population of Senecio rivularis (Waldst. et Kit.) DC. andSenecio umbrosus Waldst. et Kit., 27–39. Maria Curie-Skłodowska University Press, Lublin.
• CZARNECKA B. 2006. Large-scale vs. small-scale factors affecting flowering patterns in Senecio macrophyllusM. Bieb., a long-lived perennial, Acta Agrobotanica 59:233–239.
• DHAKA N, and KOTHARI SL. 2005. Micropropagation of Eclipta alba (L.) Hassk – an important medicinal plant. In VitroCellular and Developmental Biology – Plant 41:658–661.
• EMMANUEL S, IGNACIMUTHU S, and KATHIRAVAN K. 2000. Micropropagation of Wedelia calendulacea Less., amedicinal plant. Phytomorphology 50: 195–200
• EVENOR D, and REUVENI M. 2004. Micropropagation of Achillea filipendulina cv. 'Parker'. Plant Cell, Tissue and OrganCulture 79: 91–93.
• GERTSSON UE. 1988. Large-scale in vitro propagation of Senecio x hybridus Hyl. Journal of HorticulturalScience 63: 131–136.
• GRUBISIĆ D, SAVIKIN-FODULOVIC K, MISIC D, GIBA Z, and KONJEVIC R. 2004. In vitro stem elongation of stemless carlinethistle. Plant Growth Regulation 44: 65–69.
• HAZARIKA BN. 2003. Acclimatization of tissue-cultured plants. Current Science 85: 1704–1712.
• JOSHI M, and DHAR U. 2003. In vitro propagation of Saussurea obvallata (DC.) Edgew. – an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Reports 21:933–939.
• KIM KH, PARK HK, PARK MS, and YEA UD. 2001. Effects of auxin and cytokinin on organogenesis of soybean Glycine maxL. Journal of Plant Biotechnology 3: 95–100.
• KORACH A, JULIANI HR, KAPTEYN J, and SIMON JE. 2002. In vitro regeneration of Echinacea purpurea from leaf explants.Plant Cell, Tissue and Organ Culture 69: 79–83.
• MINOCHA SC. 1987. Plant growth regulators and morphogenesis in cell and tissue culture of forest trees. In: Bonga JM,Durjan DJ [eds.], Cell and Tissue Culture in Forestry, vol. 1, 50–66. Martinus Nijhoff Publishers,Dordrecht.
• MISRA P, and DUTTA SK. 1999 In vitro propagation of white marigold (Tagetes erecta L.) through root tip proliferation. Current Science 77: 1138–1140.
• MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 437–497.
• POONAWALA IS, JANA MM, and NADGAUDA RS. 1999. Factors influencing bud break and rooting and mass-scale micropropagationof three Phragmites species: P. karka,P. communis and P. australis. Plant Cell Reports 18:696–700.
• SUJATHA M. 1997. In vitro adventitious shoot regeneration for effective maintenance of male sterile niger [Guizotia abyssinica (L.f.) Cass.]. Euphytica 93: 89–95.
• SUJATHA M, and REDDY TP. 1998. Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Reports17: 561–566.
• SUJATHA G, and RANJITHA KUMARI BD. 2007. Effect of phytohormones on micropropagation of Artemisia vulgaris L.Acta Physiologica Plantarum 29: 189–195.
• TUTIN FG, HEYWOOD VH, BURGES NA, MOORE DM, VALENTINE DH, WALTERS SM, and WEBB DA. 1976. Flora Europea, vol. 4.Plantaginaceae to Compositae (and Rubiaceae), 196.Cambridge University Press, Cambridge – New York-Melbourne.
• TIWARI V, TIWARI KN, and SINGH BD. 2001. Comparative of cytokinins on in vitro propagation of Bacopa monniera.Plant Cell, Tissue and Organ Culture 66: 9–16.
• TREJGELL A, DĄBROWSKA G, and TRETYN A. 2009. In vitro regeneration of Carlina acaulis subsp. simplex from seedlingexplants. Acta Physiologica Plantarum 31: 445–453.
• VANEGAS PE, CRUZ-HERNANDEZ A, VALVERDE ME, and PEREDESLÓPEZ O. 2002. Plant regeneration via organogenesis inmarigold. Plant Cell, Tissue and Organ Culture 69: 279–283.