Micropropagation of Carlina acaulis L.

• Autorzy: Trejgell A , Bednarek M. , Tretyn A.
• Języki publikacji: EN

Abstrakty

EN

An efficient shoot propagation system for Carlina acaulis was developed in this study. The experimental material consisted of shoot tips and fragments of hypocotyls excised from 10-day-old seedlings. The explants were transferred to proliferation medium supplemented with different types of cytokinins: 6-benzylaminopurine (BA, 4.4 or 13.3 µM), kinetin (Kn, 4.7 or 13.9 µM) and zeatin (Zea, 4.6 or 13.7 µM) in combination with naphthaleneacetic acid (0.54 µM NAA). The morphogenetic response was best in culture on medium supplemented with 13.3 µM BA, and shoot organogenesis frequency was highest for shoot tips (100%). On average, 7.5 shoots were induced per explant of the initial material, and the multiplication rate in five subsequent subcultures was 6.1. Shoot length was lower in culture with BA in the medium than with Kn or Zea. Plantlets rooted with 60% frequency in vitro on full-strength MS medium and with 55.3% frequency ex vitro. Reduction of the mineral salt concentration (1/2MS) stimulated rhizogenesis. Addition of auxins stimulated both the frequency and number of roots per shoot, but only in combination with full-strength MS medium. Regenerated plants were able to flower and gave viable seeds.

Słowa kluczowe

EN

shoot; Carlina acaulis; benzylaminopurine; hypocotyl; stemless carline thistle; Compositae; shoot propagation system; micropropagation; perennial plant

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2009
• Tom: 51
• Numer: 1
• Opis fizyczny: p.97-103,fig.,ref.

Twórcy

autor

Trejgell A

• Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

autor

Bednarek M.

autor

Tretyn A.

Bibliografia

• Baskaran P, and Jayabalan N. 2005. An efficient micropropagation system for Eclipa alba - a valuable medicinal herb. In Vitro Cellular and Developmental Biology - Plant 41: 532-539.
• Chaudhuri KN, Ghosh B, and Jha S. 2004. The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica. Plant Cell Reports 22: 731-740.
• Ćurković Perica M. 2003. In vitro propagation of Centaurea rupestris L. Acta Biologica Cracoviensia Series Botanica 45: 127-130.
• Dhaka N, and Kothari SL. 2005. Micropropagation of Eclipta alba (L.) Hassk - an important medicinal plant. In Vitro Cellular and Developmental Biology - Plant 41: 658-661.
• Emmanuel S, Ignacimuthu S, and Kathiravan K. 2000. Micropropagation of Wedelia calendulacea Less., a medicinal plant. Phytomorphology 50: 195-200.
• Galek R, and Kukułczanka K. 2000. Propagation of Carlina acaulis in vitro culture methods. IX All-Poland Conference In Vitro Cultures and Plant Biotechnology. 10-13.09.2000, Gdańsk - Sobieszewo, 132.
• Grubisić D, Savikin-Fodulovic K, Misic D, Giba Z, and Konjevic R. 2004. In vitro stem elongation of stemless carline thistle. Plant Growth Regulation. 44: 65-69.
• Joshi M, and Dhar U. 2003. In vitro propagation of Saussurea obvallata (DC.) Edgew. - an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Reports 21: 933-939.
• Kim KH, Park HK, Park MS, and Yea UD. 2001. Effects of auxin and cytokinin on organogenesis of soybean Glycine max L. Journal of Plant Biotechnology 3: 95-100.
• Korach A, Juliani HR, Kapteyn J, and Simon JE. 2002. In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell, Tissue and Organ Culture 69: 79-83.
• Lisowska K, and Wysokinska H. 2000. In vitro propagation of Catalpa ovata G. Don. Plant Cell, Tissue and Organ Culture 60: 171-176.
• Liu Z, and Li Z. 2001. Micropropagation of Camptotheca acuminata Decaisne from axillary buds, shoot tips, and seed embryos in a tissue culture system. In Vitro Cellular and Developmental Biology - Plant 37: 84-88.
• Martin KP. 2003. Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Reports 21: 415-420.
• Misra P, and Dutta SK. 1999. In vitro propagation of white marigold (Tagetes erecta L.) through shoot tip proliferation. Current Science 77: 1138-1140.
• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physlologia Plantarum 15: 437-497.
• Poonawala IS, Jana MM, and Nadgauda RS. 1999. Factors influencing bud break and rooting and mass-scale micropropagation of three Phragmites species: P. karka, P. communis and P. australis. Plant Cell Reports 18: 696-700.
• Rybczyński JJ, and Mikuła A. 2006. Engagement of biotechnology in protection of threatened plant species in Poland. Biodiversity Research and Conservation 3-4: 361-368.
• Scarpa GM, Milia M, and Satta M. 2000. The influence of growth regulators on proliferation and rooting of in vitro propagation myrtle. Plant Cell, Tissue and Organ Culture 62: 175-179.
• Sujatha M. 1997. In vitro adventitious shoot regeneration for effective maintenance of male sterile niger (Guizotia abyssunica (L.f.) Cass.). Euphytica 93: 89-95.
• Sujatha M, and Reddy TP. 1998. Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Reports 17: 561-566.
• Trejgell A, Dąbrowska G, and Tretyn A. 2009. In vitro regeneration of Carlina acaulis subsp. simplex from seedling explants. Acta Physiologiae Plantarum 31: 445-453. DOI: 10.1007/s11738-008-0252-5
• Tutin FG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, and Webb DA. 1976. Flora Europea (Plantaginaceae to Compositae and Rubiaceeae), Vol. 4: 210. Cambridge University Press, Cambridge - New York - Melbourne.
• Yucesan B, Turke AU, and Gurel E. 2007. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell, Tissue and Organ Culture 91: 243-250.
• Zając A, and Zając M. 2001. Distribution Atlas of Vascular Plants in Poland. Institute of Botany of Jagiellonian University, Krakow.
• Zarzycki K, and Szeląg Z. 2006. Red list of the vascular plants in Poland. In: Mirek Z [ed.]. Red List Plants and Fungi in Poland, 9-20. Szafer Institute of Botany, Polish Academy of Sciences, Krakow.