Tissue-specific expression of 14-3-3 isoforms during barley microspore and zygotic embryogenesis

• Autorzy: De F. M. S. , Louwerse J.D. , Lamers G.E.M. , Spaink H.P. , Wang M.
• Języki publikacji: EN

Abstrakty

EN

Conserved 14-3-3 proteins have been shown to play regulatory roles in eukaryotic cells, including cell cycle control and differentiation. We were interested in the possible involvement of 14-3-3 proteins in the embryogenic process of barley (Hordeum vulgare L.). Barley microspore-derived embryo development was used as a model system. Immunolocalization of three barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, was carried out using isoform-specific antibodies. In immature microspore-derived embryos, 14-3-3C was specifically expressed underneath the L1 layer of the shoot apical meristem and in the scutellum. Comparative studies showed that 14-3-3C was also expressed underneath the L1 layer of the shoot apical meristem and in the scutellum of immature zygotic embryos. We further demonstrated that 14-3-3C expression is restricted to L2 layer-derived cells of in vitro shoot meristematic cultures. Our results reveal that 14-3-3C isoform tissue-specific expression is closely related to defined events during differentiation processes in embryogenesis and in vitro meristematic cultures.

Słowa kluczowe

EN

cell cycle; 14-3-3 protein; 30 kD protein; eukaryotic organism; zygotic embryogenesis; microspore; eukaryotic cell; botany; isoform; barley; embryogenesis; Hordeum vulgare; plant; differentiation

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2003
• Tom: 45
• Numer: 1
• Opis fizyczny: p.103-106,fig.

Twórcy

autor

De F. M. S.

• Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

autor

Louwerse J.D.

autor

Lamers G.E.M.

autor

Spaink H.P.

autor

Wang M.

Bibliografia

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