Porownanie metod szachownicy i E-testu stosowanych do badania przeciwbakteryjnej aktywnosci zestawien dwoch antybiotykow

• Autorzy: Grzybowska W. , Banaszczyk-Rus M. , Tyski S.

Warianty tytułu

EN

Comparison of checkerboard and E-test methods used for analysis of two antibiotics combination

• Języki publikacji: PL

Abstrakty

PL

Stosując dwie metody oceny łącznego oddziaływania antybiotyków - klasyczną metodę szachownicy oraz nową metodę opartą o E-testy, uzyskano porównywalne wyniki oznaczeń przeciwbakteryjnej aktywności zestawień aminoglikozydów z drugim antybiotykiem.

EN

The aim of this study was to compare checkerboard method with E-test assay for interaction analysis of aminoglycosides in combination with other antibiotics on selected clinical bacterial strains. In the first step, MIC values of analysed antibiotics, against particular bacterial strain were established. In the next step, antibiotics interaction was analysed by checkerboard technique and E-test. We found some difficulties while comparing these two methods. The checkerboard and E-test results corresponded in about 55%. Twenty-one percentage of results obtained by both methods showed some discrepancies. In 15% of cases, because of high MICs values, comparison of the results was impossible. Some investigators declare FIC indexes, from over 0.5 up to 4, for neutral effects. Sharing this point of view, above 21% of discrepancies results agreed. In such situation, definite disagreement was observed only in 8% of obtained results. In this investigation, additive and neutral effects were dominant. The E-test technique is less-laborous than standard agar method. In this study the E-test assay indicated synergy in only one case. Because of the manner in which the E-test strips were placed on the agar (scales intersecting at the MICs) only dramatic cases of antagonism were detected. Mild cases were undetected because the inhibition zone run under the crossed strips and was therefore unreadable and interpreted as indifference. On the basis of these results, examination of interactions between antibiotics by E-test, appears to be possible alternative to checkerboard method with mentioned limitations.

Słowa kluczowe

PL

szczepy bakteryjne; antybiotyki; oddzialywanie laczne antybiotykow; antybiotyki aminoglikozydowe; aktywnosc antybakteryjna; metody oceny; test E-test; metoda szachownicy

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 1
• Opis fizyczny: s.65-75,rys.,tab.,bibliogr.

Twórcy

autor

Grzybowska W.

• Narodowy Instytut Zdrowia Publicznego w Warszawie, ul.Chelmska 30/34, 00-725 Warszawa

autor

Banaszczyk-Rus M.

autor

Tyski S.

Bibliografia

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• 2. Cappelletty DM, RybakMJ. Comparision of methodologies for synergism testing of drug combinations against resistant strains of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1996; 40: 677-83.
• 3. Grzybowska W, Wójcik A, Tyski S. Ocena oddziaływania neomycyny z antybiotykiem innej grupy na wybrane szczepy bakteryjne. Med Dośw Mikrobiol 2004; 56: 187-98.
• 4. Grzybowska W, Вanaszczyk-Ruś M, Tyski S. Ocena oddziaływania aminoglikozydu z antybiotykiem innej grupy na wybrane szczepy bakteryjne. Med Dośw Mikrobiol 2004; 56: 275-85.
• 5. Grzybowska W, Вanaszczyk-Ruś M, Wójcik A, Tyski S. Porównanie metod szachownicy i „time-kill" stosowanych do badania zestawienia dwóch antybiotyków. Med Dośw Mikrobiol 2004;56: 391-403.
• 6. Howe RA, WoottonM, Bennett PM, MacGowan АР, Walsh TR. Interactions between methicillin and vancomycin in methicillin-resistant Staphylococcus aureus strains displaying different phenotypes of vancomycin susceptibility. J Clin Microbiol 1999; 37: 3068-71.
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• 8. White RL, Burgess DS, Manduru M. i inni. Comparision of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test. Antimicrob Agents Chemother 1996; 40: 1914-8.