Wspolczesne metody immunochemiczne umozliwiajace okreslenie gatunku pszenicy Triticum aestivum i Triticum durum

• Autorzy: Makowska A. , Obuchowski W.
• Języki publikacji: PL

Abstrakty

EN

The potential of immunochemical methods to be applied for the classification of wheat species was verified. The experimental material was: common wheat grain of different cultivars, grain of the cross common by durum wheat, durum wheat grain and wheat grain samples of unknown species. Pasta samples of different common wheat flour content were also tested. Three immunochemical kits for wheat species classification were used: Durotest P, Pasta Quality Control (the quantity tests - ELISA) and membrane kit - Durotest S. The results indicate that the absorbance values depend on the cultivar of common wheat grain. In the case of the cross common by durum wheat the results are similar to those obtained for common wheat grain. Those immunochemical methods allow the discrimination between common and durum wheat grain quickly and unmistakably. The results of quantity tests show that it is possible to determine common wheat flour content in pasta products at the level of 2%.

Słowa kluczowe

PL

metody immunochemiczne; Triticum aestivum; zboza; Triticum durum; pszenica; identyfikacja

• Wydawca: -
• Czasopismo: Pamiętnik Puławski
• Rocznik: 2004
• Tom: 135
• Opis fizyczny: s.171-179,rys.,tab.,bibliogr.

Twórcy

autor

Makowska A.

• Akademia Rolnicza, Poznan

autor

Obuchowski W.

Bibliografia

• 1. Autran J.C., Barnwell P., Bony M., Landi A., Lumley I., Griffin M., Paraf A., Resmini P., Stimson W., Violle P.: Determination of Common Wheat in Pasta Products Dried at Elevated Temperatures. Fin. Rep. Mes. and Test. Progr. (BCR), 1994, 1-85.
• 2. BioKits. Pasta Quality Control for the Detection of Common Wheat in Durum Wheat Pasta Products by Enzyme Immunoassay. Cat. No. 902058M. 2000. Tepnel BioSystems Ltd, Flintshire, U.K. 1-12.
• 3. Brett G. M., Mills E. N. C., Parmar S , Tatham A. S., Shewry A.P.R., Morgan M.R.A.: Monoclonal Antibodies that Recognise the Repeat Motif of the S-Poor Prolamins. J. Cereal Sci., 1993, 12: 245-255.
• 4. Brzeziński W. J.: Identyfikacja pszenicy durum oraz określanie domieszek pszenicy zwyczajnej w makaronach. Przegl. Zboż.-Mlyn., 1996, 6: 37-38.
• 5. Dawood M .R., Howes N.K., Bushuk W.: Preparation of Monoclonal Antibodies against Specific Gliadin Proteins and Preliminary Investigation of Their Ability to Discriminate Cereal Cultivars. J. Cereal Sci., 1989, 10: 105-112.
• 6. Denery-Papini S., Samson M.F., Autran J.C.: Anti-peptide Antibodies Directed against Omega-gliadins for the Detection of Sequences from Bread and Durum Wheat. Food Agric. Immunol., 2000, 12: 67-75.
• 7. Durotest P. Plate Format - Durum Purity Test for Pasta. 1999 Rhone Diagnostics Technologies Ltd, Scotland, 1-4.
• 8. Durotest S. For the Detection of Non-durum Wheat Adulterants in Semolina. 1999. Rhone Diagnostics Technologies Ltd, Scotland, 1-5.
• 9. Gąsiorowski H., Obuchowski W.: Pszenica makaronowa durum (Triticum durum). Post. Nauk Rol., 1978, 1: 35-52.
• 10. Gilles K .A , Youngs V.L.: Lipids of Durum Wheat and Their Role in Distinguishing Durum from Common Wheats. Macaroni J., 1969, 51: 12-15.
• 11. Kmieciak S.: Wykrywanie dodatku mąki z pszenicy zwyczajnej w makaronach. Cz. II. Badanie makaronów metodą chromatografii cieczowej i immunochemiczną. Przegl. Zboż.-Młyn, 1999, 2: 9-12.
• 12. Obuchowski W.: Technologia przemysłowej produkcji makaronu. Skrypt AR Poznań, 1997.
• 13. Obuchowski W., Mitręga A.: Określanie zawartości mąki z innego surowca niż pszenica durum w makaronach metodą “Durotest”. Przegl. Zboż.-Młyn, 1998, 9: 62-63.
• 14. Obuchowski W., Zdziarski R., Krupa E.: Metody określające obecność mąki z pszenicy zwyczajnej w makaronach. Przegl. Zboż.-Młyn, 1990, 34: 5-6.
• 15. Skerritt J.H., Hill A.S., Andrews J.L.: Antigenicity of Wheat Prolamins: Detailed Epitope Analysis Using a Panel of Monoclonal Antibodies. J. Cereal Sci, 2000, 32: 259-279.
• 16. Strebeyko P.: Biologia pszenicy. PWN, Warszawa, 1976.