Occurrence of main Marek's disease genes during infection of chickens

• Autorzy: Wozniakowski G , Samorek-Salamonowicz E. , Kozdrun W.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was the determination of the occurence of ICP4, pp38, meq, and LAT genes of Marek's disease virus (MDV) and its rate of replication in internal organs of infected chickens. The genes were determined using PCR, while the virus replication was measured by real-time PCR method. The results have shown the presence of ICP4 and pp38 genes starting from the 3rd d p.i. while meq oncogene was detected from the 7 d p.i. During the whole period of the experiment, no presence of latency associated transcript gene (LAT) was found. The virus replicated most intensively in the spleen and bursa of Fabricius then in the thymus, liver, and lungs. The results imply a role of the main genes ICP4 and pp38 in early cytolytic infection of chickens, and the further occurrence of meq oncogene associated with tumourgenesis. The highest dynamics of MDV replication in the lymphoid organs - bursa of Fabricius, thymus, and spleen indicates its stringent association with lymphoid cells and tissues.

Słowa kluczowe

PL

Marek's disease; Marek's disease virus; virus replication; animal disease; chicken; infection; gene; ICP4 gene; pp38 gene; meg gene; LAT gene; polymerase chain reaction; Mardivirus; Alphaherpesvirinae

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 2
• Opis fizyczny: p.123-127,fig.,ref.

Twórcy

autor

Wozniakowski G

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Samorek-Salamonowicz E.

autor

Kozdrun W.

Bibliografia

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