Polymorphism in the 5' flanking region of the myostatin gene affects myostatin and TGF-beta1 expression in bovine skeletal muscle

• Autorzy: Jank M. , Zwierzchowski I. , Siadkowska E. , Budasz-Swiderska M. , Sadkowski T. , Oprzadek J. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

Myostatin (GDF-8) is a key protein responsible for skeletal muscle growth and development so mutations in the mstn gene can have major economic and breeding consequences. The aim of the present study was to investigate polymorphism in the 5’flanking region of the mstn gene and its possible influence on the myostatin level in skeletal muscles of Polish Black-and-White bulls. The relation between expression of myostatin and another member of the TGF-β superfamily, TGF-β1, was also examined. We uncovered polymorphism in the 5’flanking region of the mstn gene: G/C substitution at position -7828 (relative to translation start codon ATG). The most frequent genotype was GC (43.6%), followed by genotypes CC (34.2%) and GG (22.2%). The concentration of the active form of myostatin (26 kDa) in M. semitendinosus of homozygotes (CC) was the lowest, whereas the expression of this cytokine in heterozygotes was the highest. The changes in mstn expression did not, however, influence average carcass traits (weight of valuable cuts and weight of lean in valuable cuts). The pattern of differences in the TGF-β1 concentration corresponded to that observed for myostatin.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2006
• Tom: 15
• Numer: 3
• Opis fizyczny: p.381-391,fig.,ref.

Twórcy

autor

Jank M.

• Warsaw Agricultural University, Nawoursynowska 159, 02-787 Warsaw, Poland

autor

Zwierzchowski I.

autor

Siadkowska E.

autor

Budasz-Swiderska M.

autor

Sadkowski T.

autor

Oprzadek J.

autor

Motyl T.

Bibliografia

• Budasz-Świderska M., 2005. The relationship between myostatin and transforming growth factor β1 during myogenesis in skeletal muscles. PhD. Thesis, Warsaw Agricultural University (Poland)
• Budasz-Świderska M., Jank M., Motyl T., 2005. Transforming growth factor-β1 upregulates myostatin expression in mouse C2C12 myoblasts. J. Physiol. Pharmacol. 56, Suppl. 3, 195-214
• Crisa A., Marchitelli C., Savarese M.S., Valentini A., 2003. Sequence analysis of myostatin promoter in cattle. Cytogenet. Genome Res. 102, 48-52
• Dunner S., Miranda M.E., Amigues Y., Canon J., Georges M., Hanset R., Williams J., Menissier F., 2003. Haplotype diversity of the myostatin gene among beef cattle breeds. Genet. Sel. Evol. 35, 103-118
• Grobet L., Martin M.J., Poncelet D., Pirottin D., Brouwers B., Riquet J., Schoeberlein A., Dunner S., Menissier F., Masabanda J., Fires R., Hanset R., Georges M., 1997. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat. Genet. 17, 71-74
• Grobet L., Poncelet D., Royo L.J., Brouwers B., Pirottin D., Michaux C., Menissier F., Zanotti M., Dunner S., Georges M., 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mamm. Genome 9, 210-213
• Jeanplong F., Sharma M., Paterson K.A., Morris C.A., Kambadur R., 2000. Polymorphism in dinucleotide repeat (BTAFJ1) upstream to the bovine myostatin locus. Anim. Genet. 31, 340-341
• Joulia D., Bernardi H., Garandel V., Rabenoelina F., Vernus B., Cabello G., 2003. Mechanisms involved in the inhibition of myoblast proliferation and differentiation by myostatin. Exp. Cell Res. 286, 263-75
• Kambadur R., Sharma M., Smith T.P., Bass J.J., 1997. Mutations in myostatin (GDF8) in double–muscled Belgian Blue and Piedmontese cattle. Genome Res. 7, 910-916
• Kanai N., Fujii T., Saito K., Tokoyama T., 1994. Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood. J. Clin. Pathol. 47, 1043-1044
• Klauzińska M., 2002. Polymorphism of 5’ flanking regions of GH, GH, prolactin and myostatin genes (in Polish). PhD. Thesis, Institute of Genetics and Animal Breeding, Jastrzębiec (Poland)
• Klauzińska M., Zwierzchowski L.M., Siadkowska E., Szymanowska M., Grochowska R., Żurkowski M., 2000. Comparison of selected gene polymorphisms in Polish Red cattle and Polish Black-and-White cattle. Anim. Sci. Pap. Rep. 18, 107-116
• Marchitelli C., Savarese M.C., Crisa A., Nardone A., Marsan P.A., Valentini A., 2003. Double muscling in Marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene. Mamm. Genome 14, 392-395
• McCroskery S., Thomas M., Maxwell L., Sharma M., Kambadur R., 2003. Myostatin negatively regulates satellite cell activation and self-renewal. J. Cell Biol. 162, 1135-1147
• McPherron A.C., Lee S.J., 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Nat. Acad. Sci. USA 94, 12457-12461