Usefulness of the PCR technique for bacterial DNA detection in blood of the patients after 'opened heart' operation

• Autorzy: Siondalski P , Siebert J. , Samet A. , Bronk M. , Krawczyk B. , Kur J.
• Języki publikacji: EN

Abstrakty

EN

To confirm the sensitivity of the polymerase chain reaction (PCR) technique (versus blood cultures) and to gain a better understanding of the incidence of true- and false-positive results when using this technique, one hundred randomly chosen patients treated operationally because of valve defects were examined. In our studies we found that PCR techniques using universal primers complementary to the bacterial 16S rDNA showed promise as being more sensitive than conventional blood culture (BC) techniques. From the time that a blood culture is positive, conventional methods of culture and antibiotic susceptibility testing require at least 64 h for the detection of infection or colonization. Rapid identification of bacteria from blood using PCR technique accelerates the microbiological diagnosis.

Słowa kluczowe

EN

patient; blood circulation; blood infection; polymerase chain reaction; detection; bacterial DNA; cardiac surgery

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 3
• Opis fizyczny: p.145-149,ref.

Twórcy

autor

Siondalski P

• Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland

autor

Siebert J.

autor

Samet A.

autor

Bronk M.

autor

Krawczyk B.

autor

Kur J.

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