Tryptic digestion of peptides corresponding to modified fragments of human growth hormone-releasing hormone

• Autorzy: Witkowska E. , Orlowska A. , Izdebski J.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to examine the degradation of short peptides corre­sponding to modified fragments of human growth hormone-releasing hormone by trypsin. Six analogues of pentapeptide 9-13 of human growth hormone-releasing hor­mone containing homoarginine, ornithine, glutamic acid, glycine, leucine or phenyl­alanine residue in position 11, two analogues of hexapeptide 8-13 of human growth hormone-releasing hormone and two analogues of heptapeptide 7-13 of human growth hormone-releasing hormone containing homoarginine or glycine residue in position 11 were obtained. The peptides were subjected to digestion by trypsin and the course of reaction was monitored using HPLC. It was found that the rate of hy­drolysis of the Lys12-Val13 peptide bond depends on the amino-acid residue preceding Lys12 . The extension of the peptide chain towards the N-terminus by introduction of consecutive amino-acid residues corresponding to the human growth hormone-re­leasing hormone sequence accelerates the hydrolysis process. These results may be of assistance in designing new analogues of human growth hormone-releasing hor­mone, more resistant to the activity of proteolytic enzymes.

Słowa kluczowe

EN

hormone secretion; man; peptide; RNA synthesis; important regulator; tryptic digestion; growth hormone

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 1
• Opis fizyczny: p.51-56,ref.

Twórcy

autor

Witkowska E.

• Warsaw University, L.Pasteura 1, 02-093 Warsaw, Poland

autor

Orlowska A.

autor

Izdebski J.

Bibliografia

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