Otrzymywanie przeciwcial monoklonalnych do diagnostyki pomoru klasycznego swin

• Autorzy: Markowska-Daniel I , Pejsak Z.
• Języki publikacji: PL

Abstrakty

EN

Monoclonal antibodies (MAbs) for diagnosis of classical swine fever have been produced. Hog cholera virus, the Alfort strain, was used as an antigen for the immunization of mice; it was propagated in the culture of primary swine kidney cells. Mice of the BALB-c strain were immunized in four cycles and the spleen cells of the mice characterized by the highest titers of antibodies were taken for hybridization. The spleen cells were fused with S p2/o myeloma cells in the presence of PEG 4000. The hybrydoma cells were cultivated in a 20 per cent DMEM HY-HT medium at 37°C with a 5 per cent presence of CO2. The supernatant fluids were tested for the presence of specific antibodies by ELISA and NPLA assays. The selected cultures were subcloned twice in a semi-solid agar, then they were isotyped using the Immuno-Select TM set. Intraperitoneal inoculation of hybri- doma cells male BALB/c mice resulted in the production of ascites fluids containing MAbs. The ascites fluids were purified by means of an Econo-Pac Protein A Kit and conjugated with horse-radish peroxidase.

Słowa kluczowe

PL

przeciwciala monoklonalne; diagnostyka serologiczna; diagnostyka wirusologiczna; diagnostyka; pomor swin; otrzymywanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1994
• Tom: 50
• Numer: 04
• Opis fizyczny: s.159-161,bibliogr.

Twórcy

autor

Markowska-Daniel I

• Instytut Weterynarii, Al.Partyzantow 57, 24-100 Pulawy

autor

Pejsak Z.

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