Analiza metoda RAPD genetycznej zmiennosci szczepow z rodzaju Bordetella

• Autorzy: Mosiej E. , Gzyl A. , Slusarczyk J.

Warianty tytułu

EN

Genetic diversity analysis of isolates belonging to Bordetella genus

• Języki publikacji: PL

Abstrakty

PL

W pracy zastosowano technikę RAPD (Random Amplified Polymorphic DNA) w klasyfikacji gatunkowo swoistej oraz w ocenie zmienności wewnątrzgatun- kowej szczepów 8 gatunków z rodzaju Bordetella. Przeprowadzona optymalizacja reakcji RAPD umożliwiła uzyskanie powtarzalnych i różnicujących profili RAPD. Wykazano przydatność metody RAPD w identyfikacji gatunkowej szczepów B. avium i B. holmesii oraz w różnicowaniu wewnątrzgatunkowym szczepów B. bronchiseptica i B. hinzii.

EN

In this study, Random Amplified Polymorphic DNA (RAPD) method was used to track differences among human and animals isolates of eight known species of Bordetella genus. One hundred representative strains of these species from international and Polish bacterial collections were genotyped according to RAPD protocols using primer 1254 or 1247. Problems with reproducibility and discriminatory power, frequently discussed in literature, have been overcome by precise optimization procedure, which allowed to achieve reliable conditions for Bordetella species analysed. This study proved high potential of RAPD method using primers 1247/1254 for intra-species differentiation of B. bronchiseptica and B. hinzii isolates. Additionally RAPD method has been found to be useful for confirming B. avium and B. holmesii identification.

Słowa kluczowe

PL

szczepy bakteryjne; Bordetella; bakterie; zmiennosc wewnatrzgatunkowa; oznaczanie; metoda RAPD; Bordetella avium; Bordetella holmesii; Bordetella bronchiseptica; Bordetella hinzii

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 4
• Opis fizyczny: s.395-407,rys.,tab.,bibliogr.

Twórcy

autor

Mosiej E.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Gzyl A.

autor

Slusarczyk J.

Bibliografia

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