Detection of Babesia bigemina antibodies by Dot-ELISA in cattle and buffaloes

• Autorzy: Mishra A K , Reddy G G B , Rao J R , Tewari A K
• Języki publikacji: EN

Abstrakty

EN

An affinity purified Babesia bigemina piroplasm antigen was successfully employed in a Dot-ELISA for detection of antibodies in buffaloes and catle. A low degree of cross-reactivity was seen against putative B. bigemina (Mexican) and B. bovis undiluted reference serum and no cross-reactivity was observed in seru dilutions of 1:10 and above. Theileria annulata did not show any reactivity with B. bigemina. The assay detected antibodies at 7, 14 or 21 days post-experimental infection in bovine calves with 37.5, 87.5 and 100% seroreactivity, respectively. The incidence of B. bigemina infections was found highest in cattle (19.91) as against 7.4% in buffaloes out of 486 sera samples, examined from Boophilus microplus infested areas from Orissa, Rajasthan and Uttar Pradesh States.

Słowa kluczowe

EN

Boophilus microplus; disease; infection; serum; blood; bovine calf; antibody; antigen; babesiosis; cattle; buffalo; Babesia bigemina; India; Theileria annulata; Babesia bovis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1998
• Tom: 43
• Numer: 1
• Opis fizyczny: p.43-45

Twórcy

autor

Mishra A K

• Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243122, India

autor

Reddy G G B

• Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243122, India

autor

Rao J R

• Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243122, India

autor

Tewari A K

• Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243122, India

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