IRAP and REMAP assessments of genetic similarity in rice

• Autorzy: Branco C J S , Vieira E A , Malone G , Kopp M M , Malone E , Bernardes A , Mistura C C , Carvalho F I F , Oliveira C A
• Języki publikacji: EN

Abstrakty

EN

Rice is a model genome for cereal research, providing important information about genome structure and evolution. Retrotransposons are common components of grass genomes, showing activity at transcription, translation and integration levels. Their abundance and ability to transpose make them good potential markers. In this study, we used 2 multilocus PCR-based techniques that detect retrotransposon integration events in the genome: IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism). Markers derived from Tosl 7, a copia-like endogenous retrotransposon of rice, were used to identify genetic similarity among 51 rice cultivars (Oryza saliva L.). Genetic similarity analysis was performed by means of the Dice coefficient, and dendrograms were developed by using the average linkage distance method. A cophenetic correlation coefficient was also calculated. The clustering techniques revealed a good adjustment between matrices, with correlation coefficients of 0.74 and 0.80, or lower (0.21) but still significant, between IRAP and REMAP-based techniques. Consistent clusters were found for Japanese genotypes, while a subgroup clustered the irrigated Brazilian genotypes.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2007
• Tom: 48
• Numer: 2
• Opis fizyczny: p.107-113,fig.,ref.

Twórcy

autor

Branco C J S

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Vieira E A

• Embrapa Cerrados, Brazil

autor

Malone G

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Kopp M M

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Malone E

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Bernardes A

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Mistura C C

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Carvalho F I F

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

autor

Oliveira C A

• Eliseu Maciel School of Agronomy, Federal University of Pelotas, Pelotas, RS-Brazil

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