Hydrogen fluoride effects on plasma membranes sterol and phospholipid composition in needles of eastern white pine [Pinus strobus L.] seedlings pretreated with different photoperiod conditions

• Autorzy: Rakowski K. J.
• Języki publikacji: EN

Abstrakty

EN

Eastern white pine (Pinus strobus L.) seedlings were pretreated with 12 h photoperiod to induce dormancy or with 18 h photoperiod to accelerate growth. Dormant and non-dormant plants were fumigated with 0.5 ppb (0.4 µg m-3) or 2.0 ppb (1.6 µg m-3) hydrogen fluoride (HF) for 2-28 days. Plasma membranes were isolated from needles of HF treated and control seedlings to determine their particular sterol and phospholipid composition. For all determinations, only those plants which did not show needle necrosis were selected. For detailed analyses, content of three main sterol (campesterol, stigmasterol, sitosterol) and phospholipid (18:1, 18:2,18:3) were selected. Analyses of plasma membrane sterol indicated significant increase in sitosterol content observed after 2 days in non- dormant and after 28 days in dormant plants. Analyses of plasma membrane phospholipid fatty acids indicate most significant increase in oleic acid (18:1) level in non-dormant seedlings after 2 and 8 days of HF treatment. Dormant plants expressed decreased level of all analysed fatty acids (18:1, 18:2, 18:3). Results obtained indicate the possibility of direct hydrogen fluoride effect on analysed fatty acid level resulting in plasma membrane phospholipid level decline during plant dormancy. On the other hand, increased sitosterol and oleic acid level suggest more specific fluoride effects on particular sterol and phospholipid synthesis in non- dormant seedlings.

Słowa kluczowe

EN

plasma membrane; pine; white pine; needle; hydrogen fluoride effect; fluoride; sterol; seedling; Pinus strobus; composition; phospholipid

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1997
• Tom: 02
• Numer: 2
• Opis fizyczny: p.161-173,fig.

Twórcy

autor

Rakowski K. J.

• Forest Research Institute, Bitwy Warszawskiej 1920R-3, 00-973 Warsaw, Poland

Bibliografia

• 1. Ratajczak, R. and Fischer-Schliebs, E. Control of higher plant activities by electrochemical proton gradient set up by H+ pumps at the plasma membrane and the tonoplast. Submited.
• 2. Palmgren, M.G. Regulation of plant plasma membrane H+-ATPase activity. Physiol. Plant. 83 (1991) 314-323.
• 3. Michelet, B. and Boutry, M. The Plasma Membrane H+-ATPase. A Highly Regulated Enzyme with Multiple Physiological Functions. Plant Physiol. 108 (1995) 1-6.
• 4. Chonn, A., Semple, S.C. and Cullins, P.R. Protein-membrane interactions in the complex of biological milieu, in: Biological Membranes: Structure, Biogenesis and Dynamics, Op den Kamp, ed. Springer-Verlag, New York, London, Paris, Tokyo, Hong Kong, Barcelona, Budapest 1994, 101-114.
• 5. Steer, M.W. Plasma membrane turnover in plant cells. J. Exp. Bot. 39 (1988) 987-996.
• 6. Leshem, Y.Y., Shewfelt, R.L., Willmer, C.M. and Pantoja, O. Plant membranes. A biophysical approach to structure, development and senescence, Kluwer Academic Publishers, Dordrecht Boston London 1992, 62-63.
• 7. Wolfenden, J. and Wellburn, A.R. Effects of summer ozone on membrane lipid composition in Norway spruce (Picea-Abies). New Phytol. 118 (1991) 323-330.
• 8. Cooper, R.A. and Strauss, J.F. Regulation of cell membrane cholesterol. in: Physiology of Membrane Fluidity, Shinizky, ed. Vol. 1. CRC Press, Boca Raton 1985, 74-98.
• 9. Guye, M.G. Sterol composition in relation to chill-sensitivity in Phaseolus spp. J. Exp. Bot. 39 (1988) 1091-1096.
• 10. Weinstein, L.H. Fluoride and plant life. J. Occup. Med. 19 (1977) 49-78.
• 11. Treshow, M. and Anderson, F.K. Plant stress from air pollution. John Wiley & Sons, Toronto. 1989.
• 12. Rakowski, K.J. and Zwiazek, J.J. Early effects of hydrogen fluoride on water relations, photosynthesis and membrane integrity in eastern white pine (Pinus strobus) seedlings. Environ. Exp. Bot. 32 (1992) 377-382.
• 13. Rakowski, K.J., Zwiazek, J.J. and Sumner, M.J. Hydrogen fluoride effects on plasma membrane composition, ATPase activity and cell structure in needles of eastern white pine (Pinus strobus) seedlings. Trees 9 (1995) 190-194.
• 14. Rakowski, K.J. Hydrogen fluoride effects on plasma membrane composition and ATPase activity in needles of white pine (Pinus strobus) seedlings pretreated with 12 h photoperiod. Trees 11 (1997) 248-253.
• 15. Hellergren, J., Widell, S., Lundborg, T. and Kylin, A. Frosthardiness development in Pinus svlvestris: The involvement of a K+-stimulated Mg2+-dependent ATPase from purified plasma membranes of pine. Physiol. Plant. 58 (1983) 7-12
• 16. Petterson, G.L. A simplification of the protein assay method of Lowry et al. which is generally more applicable. Anal. Biochem. 83 (1977) 346-351.
• 17. Zwiazek, J.J. and Shay, J.M. The effects of sodium fluoride on cytoplasmic leakage and the lipid and fatty acid composition of jack pine (Pinus banksiana) seedlings. Can. J. Bot. 66 (1988) 535- 541.
• 18. Zwiazek, J.J. and Blake, T.J. Effect of preconditioning on electrolyte leakage and lipid composition in black spruce (Picea mariana) stressed with polyethylene glycol. Physiol. Plant. 79 (1990) 71-77.
• 19. Khan, A.A. and Malhotra, S.S. Effects of agueous sulphur dioxide on pine needles glycolipids. Phytochem. 16 (1977) 539-543.