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2006 | 62 | 05 |

Tytuł artykułu

Wlasciwosci biochemiczne krajowych izolatow Streptococcus suis

Warianty tytułu

EN
Biochemical features of Polish isolates of Streptococcus suis

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to evaluate biochemical features of 575 Polish isolates of S. suis from pigs with symptoms of streptococcosis and to improve methods of identification of the pathogen. The following tests were used to evaluate biochemical features of the strains: raffinose, inulin, mannitol, arginine and esculin fermentation, and production of acetoin, β-glucuronidase as well as amylase (on starch agar plates). Typical reactions were observed in 538 isolates (94%). The highest number of positive results was obtained in the β-glucuronidase production test in which 100% of the isolates demonstrate activity of mentioned enzyme. Since positive results were evidenced in 563 isolates (98%), raffinose fermentation was also a specific feature of the tested isolates. Less specific (93% of positive strains) results were proved for the following tests: acetoin and amylase production as well as inulin fermentation. Arginine dihydrolase activity was demonstrated in 523 tested isolates (91%) and 69% had the ability to hydrolyze esculin. The least specific result was obtained in the mannitol fermentation test: 33% of the typical reactions. 14 biochemical profiles were estimated among the analyzed isolates. 60% of the strains were classified in groups B and H. In group H only the strains with typical for S. suis pattern, reaching 22.3% of the tested isolates, were placed. This study confirmed S. suis to be highly heterogenous in biochemical profiles. In our opinion the evaluation of biochemical features is not a universal method and should not be considered as a basis for bacterial identification. This method can be used as an additional test for S. suis identification, supplemented by other, more reliable techniques.

Wydawca

-

Rocznik

Tom

62

Numer

05

Opis fizyczny

s.566-569,rys.,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

Bibliografia

  • 1. Dacko J, Osek J.: Analiza wybranych cech fenotypowych szczepów Escherichia coli izolowanych od prosiąt. Medycyna Wet. 2004, 60, 861-866.
  • 2. Devriese L. A., Ceyssens K., Hommez J., Kilpper-Balz R., Schleifer K. H.: Characteristics of different Streptococcus suis ecovars and description of a simplified identification method. Vet. Microbiol. 1991, 26, 141-150.
  • 3. Devriese L. A., Ceyssens K., Hommez J., Kilpper-Balz R., Schleifer K. H.: Identification and composition of the streptococcal and enterococcal flora of tonsils, intestines and faeces of pigs. J. Appl. Bacteriol. 1994, 77, 31-36.
  • 4. Devriese L. A., Vandamme P., Pot B., Vanrobaeys M., Kersters K., Haesebrouck F.: Differentiation between Streptococcus gallolyticus strains of human clinical and veterinary origins and Streptococcus bovis strains from the intestinal tracts of ruminants. J. Clin. Microbiol. 1998, 36, 3520-3523.
  • 5. Dibb W. L., Bottolfsen K. L.: Evaluation of Rosco diagnostic beta-glucuronidase tablets in the identification of urinary isolates of Escherichia coli. Acta Pathol. Microbiol. Immunol. Scand. 1984, 92, 261-264.
  • 6. Edberg S. C., Pittman S., Singer J. M.: Esculin hydrolysis by Enterobacteriaceae. J. Clin. Microbiol. 1977, 6, 111-116.
  • 7. Edberg S. C., Trepeta R. W., Kontnick C. M., Torres A. R.: Measurement of active constitutive beta-D-glucosidase (esculinase) in the presence of sodium desoxycholate. J. Clin. Microbiol. 1985, 21, 363-365.
  • 8. Erickson E. D., Doster A. R., Pokorny T. S.: Isolation of Streptococcus suis from swine in Nebraska. J. Am. vet. med. Ass. 1984, 185, 666-668.
  • 9. Estoepangestie S., Lammler C.: Distribution of capsular types 1 to 28 and further characteristics of Streptococcus suis isolates from various European countries. Zentbl. Bakt. 1993, 279, 394-403.
  • 10. Faclam R. R.: Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl. Microbiol. 1972, 23, 1131-1139.
  • 11. Facklam R. R., Moody M. D.: Presumptive identification of group D streptococci: the bile-esculin test. Appl. Microbiol. 1970, 20, 245-250.
  • 12. Gadelle D., Raibaud P., Sacquet E.: Beta-Glucuronidase activities of intestinal bacteria determined both in vitro and in vivo in gnotobiotic rats. Appl. Environ. Microbiol. 1985, 49, 682-685.
  • 13. Gottschalk M., Higgins R., Jacques M., Beaudoin M., Henrichsen J.: Characterization of six new capsular types (23 through 28) of Streptococcus suis. J. Clin. Microbiol. 1991, 29, 2590-2594.
  • 14. Gottschalk M., Higgins R., Jacques M., Beaudoin M., Henrichsen J.: Isolation and characterization of Streptococcus suis capsular types 9-22. J. Vet. Diagn. Invest. 1991, 3, 60-65.
  • 15. Higgins R., Gottschalk M.: Streptococcal diseases, [w:] Straw B. E., D'Allaire S., Mengeling W. L., Taylor D. J. (wyd.): Disease of swine. The Iowa State University Press, USA 1999, 277-290.
  • 16. Jayarao B. M., Oliver S. P., Matthews K. R., King S. H.: Comparative evaluation of Vitek Gram-positive identification system and API Rapid Strep system for identification of Streptococcus species of bovine origin. Vet. Microbiol. 1991, 26, 301-308.
  • 17. Markowska-Daniel I., Kowalczyk A.: Identyfikacja drobnoustrojów z gatunku Streptococcus suis testem multiplex PCR. Medycyna Wet. 2005, 61, 522-525.
  • 18. Markowska-Daniel I., Szczotka A.: Diagnostyka zakażeń Streptococcus suis u świń, [w:] Diagnostyka laboratoryjna wybranych bakteryjnych i wirusowych chorób świń. Wyd. PIWet, Puławy 2003, 39-50.
  • 19. Pejsak Z.: Zakażenia Streptococcus suis u świń. Weterynaria po dyplomie 2003, 4, 3-8.
  • 20. Pejsak Z.: Zakażenia Streptococcus suis u świń. Problemy w zakresie rozpoznawania i zwalczania. Mag. Wet. 2002, Suplement - Świnie, 77-79.
  • 21. Prieto C., Garcia F. J., Suarez P., Imaz M., Castro J. M.: Biochemical traits and antimicrobial susceptibility of Streptococcus suis isolated from slaughtered pigs. Zentralbl. Veterinarmed. B. 1994, 41, 608-617.
  • 22. Quinn P. J., Carter M. E., Markey B. K., Carter G. R.: The Streptococci and related cocci, [w:] Clinical Veterinary Microbiology, Mosby, Londyn 2000, 127-136.
  • 23. Setterstrom J. A., Gross A., Stanko R. S.: Comparison of Minitek and conventional methods for the biochemical characterization of oral streptococci. J. Clin. Microbiol. 1979, 10, 409-414.
  • 24. Tarradas C., Arenas A., Maldonado A., Luque I., Miranda A., Perea A.: Identification of Streptococcus suis isolated from swine: proposal for biochemical parameters. J. Clin. Microbiol. 1994, 32, 578-580.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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