Studying protein-DNA binding by dynamic light scattering

• Autorzy: Zhelev N. Z. , Buckle R.S. , Snyder D. , Marsh P.J.
• Języki publikacji: EN

Abstrakty

EN

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Słowa kluczowe

EN

measurement; protein-DNA interaction; dimerization; dynamic light scattering; DNA

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1996
• Tom: 01
• Numer: 2
• Opis fizyczny: p.199-202

Twórcy

autor

Zhelev N. Z.

• King's College London, 26-29 Drury Lane, London, WC2B 5RL, U.K.

autor

Buckle R.S.

autor

Snyder D.

autor

Marsh P.J.

Bibliografia

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• 9. Ferre-D’Amare. A. R., Prendergast, G. C., Edward, B. Z., Burley, S. K. (1993). Nature 363, 38-45.