Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers

• Autorzy: Konnert M
• Języki publikacji: EN

Abstrakty

EN

Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.

Słowa kluczowe

EN

international conference; Europe; forest ecosystem; plant breeding; tree; Norway spruce; Picea abies; genetic variation; Germany; isoenzyme; Bavaria; DNA marker; plant genetics; provenance

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2009
• Tom: 61 Supplement
• Opis fizyczny: p.131-136,ref.

Twórcy

autor

Konnert M

• Bavarian Office for Forest Seeding and Planting, Teisendorf, Forstamtsplatz 1, 83317 Teisendorf, Germany

Bibliografia

• Ammer Ch. 2007. Die Fichte im Zeichen des Klimawandels – vom Brotbaum zum Sorgenkind? Bayerisches Landwirtschaftliches Wochenblatt 23: 40.
• Bentele M. 2007. Trotz Klimawandels gedeihen auch künftig stabile Fichtenwälder. Bericht zu Jahrestagung der ANW Baden-Württemberg. Holz-Zentralblatt 22: 576.
• Gömöry. D. 1992. Effect of stand origin on the genetic diversity of Norway spruce (Picea abies Karst.) populations. Forest Ecology and Management 54: 215–223.
• Kölling Ch. 2007. Bäume für die Zukunft. Baumartenwahl im Zeichen des Klimawandels. LWF-aktuell 60: 35–37.
• Konnert M. 1991. Die Fichte (Picea abies L. Karst.) im Schwarzwald: Genetische Variation und Korrelationen. Forstwissenschaftliches Centralblatt 110: 84–94.
• Konnert M., Behm A. 2006. Proof of identity of reproductive material based on reference samples. Mitteilungen der Bundesforschungsanstalt für Forst- und Holzwirtschaft, Hamburg 221: 61–71.
• Konnert M., Franke A. 1991. Die Fichte (Picea abies (L.) Karst.) im Schwarzwald: Genetische Differenzierung von Beständen. Allgemeine Forstund Jagdzeitung 5/6: 100–106.
• Konnert M., Maurer W. 1995. Isozymic Investigations on Norway Spruce (Picea abies (L.) Karst.) and European Silver Fir (Abies alba Mill.): A Practical Guide to Separation Methods and Zymogram Evaluation. From the German Federal-State Working Group “Conservation of Forest Gene Resources”.
• Morgante M., Vendramin G.G. 1991. Genetic variation in Italian populations of Picea abies (L.) Karst. and Pinus leucodermis Ant. Müller. In: Genetic Variation in European Populations of Forest Trees. Starck G., Ziehe M. (eds). Sauerländer’s Verlag, Frankfurt a. Main, pp. 205–226.
• Perry J.D., Bousquet J. 1998. Sequence-tagged-site (STS) markers of arbitrary genes: development, characterization and analysis of linkage in Black spruce. Genetics 149: 1089–1098.
• Riegel R., Schubert R., Müller-Starck G., Karnosky D.F., Paule L. 2001. Genetic variation in two heavily polluted stands of Norway spruce (Picea abies (L.) Karst.) as indicated by nuclear and organelle DNA markers. Forest Science 70: 21–34.
• Ruetz W.F., Bergmann F. 1989. Möglichkeiten zum Nachweis von autochthonen Hochlagenbeständen der Fichte (Picea abies) in den Berchtesgadener Alpen. Forstwissenschaftliches Centralblatt 108, 3: 164–174.
• Ruetz W.F., Konnert M., Behm A. 1996. Sind Waldschäden auch eine Frage der Herkunft? AFZ/Der Wald 14: 759–761.
• Schmidt-Vogt H. 1976. Fichtenherkünfte (Picea abies (L.) Karst.) der Bundesrepunblik Deutschland. Allgemeine Forst- und Jagdzeitung 147: 149–163.
• Schubert R., Mueller-Starck G., Riegel R. 2001. Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea abies (L.) Karst. Theoretical and Applied Genetics 103: 1223–1231.
• Tigerstedt P.M.A. 1973. Studies on isozyme variation in marginal and central populations of Picea abies. Hereditas 75: 47–60.