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2005 | 47 | 1 |

Tytuł artykułu

Physiological responses of the eustigmatophycean Nannochloropsis salina to aqueous diesel fuel pollution

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Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The marine eustigmatophycean microalga Nannochloropsis (Monallantus) salina Hibberd was cultivated in a batch culture in the presence of various concentrations (0, 25, 50, 75 and 100%) of an aqueous extract of diesel fuel oil in order to assess the influence of the pollutant on the growth and certain physiological responses of the microalga. The growth data revealed a significant negative effect of the various pollutant concentrations on the algal cell number (p≤0.05). However, at the midlogarithmic growth phase (day 8), the algal cells were analysed for chlorophyll a, β-glucan, amino acid pool, C/N ratio and elemental composition. According to our results, N. salina was significantly affected by the pollution with regard to the different physiological parameters examined, and this significance may be negative, positive or variable. The effect of the pollutant on cellular β-glucan and the total amount of amino acids was negative;h owever, the composition of the cellular amino acid pool remained unaffected. A positive effect of the pollutant on cellular chl a and the C/N ratio was observed. In addition, the pollutant showed variable effects on the composition of different elements, as shown by energy-dispersive X-ray microanalysis. Also, an existence correlation between different elements was statistically reported.

Wydawca

-

Czasopismo

Rocznik

Tom

47

Numer

1

Opis fizyczny

p.75-92,fig.,ref.

Twórcy

  • Alexandria University, Alexandria, Egypt

Bibliografia

  • Agarwal B.L., 1988, Basic statistics, Wiley Eastern Ltd., New Delhi, 748 pp.
  • Ansari Z.A., Saldanha M.C., Rajkumar R., 1997, Effects of petroleum hydrocarbons on the growth of microalga Isicrysis sp. (Chrysophyta), Indian J. Mar. Sci., 26, 372–376.
  • Atlas R.M., Schofield E.A., Morelli F.A., Cameron R. E., 1976, Effects of petroleum pollutants on Arctic microbial populations, Environ. Pollut., 10, 35–45.
  • Barrett G.C., Elmore D.T., 1998, Amino acids and peptides, Cambridge Univ. Press, 240 pp.
  • Boussiba S., Vonshak A., Cohen Z., Avissar Y., Richmond A., 1987, Lipid and biomass production by halotolerant microalga Nannochloropsis salina, Biomass, 12, 17–24.
  • Boylan D. B., Tripp B.W., 1971, Determination of hydrocarbons in seawater extracts of crude oil and crude oil fractions, Nature, 230 (5288), 44–47.
  • Brown M.R., McCausland M.A., Kowalski K., 1998, The nutritional value of four Australian microalgal strains fed to Pacific oyster Crassostrea gigas spat, Aquaculture, 165 (3), 281–293.
  • Burkhardt S., Zondervan I., Riebesell U., 1999, Effects of CO2 concentration on C:N:P ratio in marine phytoplankton: a species comparison, Limnol. Oceanogr., 44 (3), 683–690.
  • Caldwell D.R., 1995, The effects of environmental factors on microbes, [in:] Microbial physiology and metabolism, Wm. C. Brown Publ., Dubuque, IA, 353 pp.
  • Carman K.R., Fleeger J.W., Pomarico S.M., 1997, Response of benthic food web to hydrocarbon contamination, Limnol. Oceanogr., 42 (3), 561–571.
  • Caron D.A., Peele E.R., Lim E. L., Dennett M.R., 1999, Picoplankton and nanoplankton and their trophic coupling in the surface waters of the Sargasso Sea south of Bermuda, Limnol. Oceanogr., 44 (2), 259–272.
  • Chabbi A., Rumpel C., 2004, Chemical composition of organic matter in extremely acid, lignite-containing lake sediments impacted by fly ash contamination, J. Environ. Qual., 33 (2), 628–636.
  • Czekanowski J., 1913, Zarys metod statystycznych, Anthropol. Anz., 9, 227–249.
  • DeBoer J.A., 1981, Nutrients, [in:] The biology of seaweeds, C.S. Lobban & M.J. Wynne (eds.), Blackwell Sci. Publ., Oxford, 356–392.
  • Dennington V.N., George H.M., Wybron C.H.E., 1975, The effect of oil on growth of fresh water phytoplankton, Environ. Pollut., 8, 233–237.
  • Dubois M., Gilles K.A., Hamilton J. K., Rebers P.A., Smith F., 1956, Colorimetric method for determination of sugar and related substances, Anal. Chem., 28, 350–356.
  • El-SheekhM.M., 2000, Effect of glyphosate herbicide on growth, photosynthesis and some metabolic activities on the green alga Chlorella kessleri (Chlorophyta), Egypt. J. Phycol., 1, 87–97.
  • Fabregas J., Herrero C., Veiga M., 1984, Effect of oil and dispersant on growth and chlorophyll a content of the marine microalga Tetraselmis suecica, Appl. Environ. Microbiol., 47 (2), 445–447.
  • Fogg G. E., 1995, Some comments on picoplankton and its importance in the pelagic ecosystem, Aquat. Microb. Ecol., 9 (1), 33–39.
  • Gill C. O., Ratledge C., 1972, Toxicity of n-alkanes, n-alk-1-enes, n-alkan-1-ols and n-alkyl-1-bromides towards yeasts, J. Gen. Microbiol., 72, 165–172.
  • Granum E., Myklestad S.M., 1999, Effect of NH4 assimilation on dark carbon fixation and β-1,3-glucan metabolism in the marine diatom Skeletonema costatum (Bacillariophyceae), J. Phycol., 35, 1191–1199.
  • Granum E., Myklestad S.M., 2001, Mobilization of β-1,3-glucan and biosynthesis of amino acids induced by NH+4 addition to N-limited cells of the marine diatoms Skeletonema costatum (Bacillariophyceae), J. Phycol., 37 (5), 772–782.
  • Hibberd D. J., 1981, Notes on the taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (synonym Xanthophyceae), Bot. J. Linn. Soc., 82 (2), 93–119.
  • Hibberd D. J., 1988, Eustigmatophyceae, [in:] Handbook of Protoctista, L. Margulis, J. Corliss, M. Melkonian & D. Chapman (eds.), Jones & Bartlett Publ., Boston, 30–50.
  • Hu H., Gao K., 2003, Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources, Biotechnol. Lett., 25 (5), 421–425.
  • Ikawa M., Mosley S.P., Barbero L. J., 1992, Inhibitory effects of terpene alcohols and aldehydes on growth of green alga Chlorella pyrenoidosa, J. Chem. Ecol., 18 (10), 1755–1760.
  • Kang S.X., Shen X. S., Huang Y.Y., Ju X., Wu Z.Q., 2003, SR-XRF analysis of characteristics of heavy element concentration in qingdao algae and application to monitoring oceanic pollution, Sci. Total Environ., 23 (1), 94–97.
  • Lai B., Khanna S., 1996, Mineralization of 14C octacosane by Acinetobacter calcoaceticus S30, Can. J. Microbiol., 42, 1225–1231.
  • Lubián L.M., Montero O., Moreno-Garrido I., Huertas E., Sobrino C., González -Delvalle M., Par´es G., 2000, Nannochloropsis (Eustigmatophyceae) as a source of commercially valuable pigments, J. Appl. Phycol., 12 (3)–(5), 249–255.
  • Moore S., Spackman D.H., Stein W., 1958, Chromatography of amino acids on sulphonated polystyrene resins, Anal. Chem., 30, 1185–1190.
  • Morales-Loo M.R., Goutx M., 1990, Effects of water soluble fractions of the Mexican crude oil ‘Isthmus Cactus’ on growth, cellular content of chlorophyll a, and lipid composition of planktonic microalgae, Mar. Biol., 104, 503–509.
  • Moreno-Garrido I., Blasco J., González-Delvalle M., Lubián L.M., 1998, Differences in Copper accumulation by the marine microalga Nannochloropsis gaditana, submitted to two different thermal treatments, Ecotox. Environ. Rest., 1 (1), 43–47.
  • Myklestad S., Djurhuus R., Mohus A., 1982, Demonstration of exo- (β-1,3)-Dglucanase activity in some planktonic diatoms, J. Exp. Mar. Biol. Ecol., 56, 205–211.
  • National Research Council, 1985, Oil in the Sea: inputs, fates and effects, Natl. Acad. Press, Washington, DC, 601 pp.
  • Nechev J. T., Khotimchenko S.V., Ivanova A. P., Stefanov K. L., Dimitrova-Konaklieva S.D., Andreev S., Popov S. S., 2002, Eff ect of diesel fuel pollution on the lipid composition of some wide-spread Black Sea algae and invertebrates, Z. Nat¨urforsch, 57 (C), 339–343.
  • Piehler M. F., Swistak J.G., Pinckney J. L., Paerl H.W., 1997, Sub-lethal effects of coastal petroleum pollution on Spartina alterniflora stem epiphytes, Chemosphere, 35 (11), 2665–2674.
  • Piehler M. F., Winkelmann V., Twomey L. J., Hall N. S., Currin C.A., Paerl H.W., 2003, Impact of diesel fuel exposure on the microphytobenthic community of an intertidal sand flat, J. Exp. Mar. Biol. Ecol., 297, 219–237.
  • Plante-Cuny M.R., Salen-Picard C., Grenz C., Plante R., Alliot E., Barranguet C., 1993, Experimental field study of the effect of crude oil, drill cutting and natural biodeposits on microphyto-and macrozoobenthic communities in a Mediterranean area, Mar. Biol., 117, 355–366.
  • Rebolloso-Fuentes M. M., Garciá-Sánchez J. L., Fernández-Seville J. M., Acién-Fernández F.G., Sánchez-Pérez J.A., Molina-Grima E., 1999, Outdoor continuous culture of Porphyridium cruentum in a tubular photobioreactor: quantitative analysis of the daily cyclic variation of culture parameters, J. Biotechnol., 70 (1)–(3), 271–288.
  • Riebesell U., Revill A.T., Holdsworth D.G., Volkman J.K., 2000, The effect of varying CO2 concentration on lipid composition and carbon isotope fractionation in Emiliania huxleyi, Geochim. Cosmochim. Acta, 64 (24), 4179–4192.
  • Rocha J.M., Gracia J. E., Henriques M.H., 2003, Growth aspects of the marine microalga Nannochloropsis gaditana, Biomol. Eng., 20 (4)–(6), 237–242.
  • Russell A.D., Hugo W.B., 1988, Perturbation of homeostatic mechanisms in bacteria by pharmaceutical, [in:] Homeostatic mechanisms in micro-organisms, R. Whittenbury, G. W. Gould, J. G. Banks & R. G. Board (eds.), Bath Univ. Press, Bath, 206–219.
  • Sánchez-Rodriguez I., Huerta-Diaz M.A., Choumiline E., Holguin-Quinones O., Zertuche-González J.A., 2001, Elemental concentrations in different species of seaweeds from Loreto Bay, Baja California Sur, Mexico: implications for the geochemical control of metals in algal tissue, Environ. Pollut., 114 (2), 145–160.
  • Schoen S., 1988, Cell counting, [in:] Experimental phycology: a laboratory manual, C. S. Lobban, D. J. Chapman & B.P. Kremer (eds.), Cambridge Univ. Press, New York, 16–22.
  • Sierra-Alvarez R., Lettinga G., 1991, The effect of aromatic structure on the inhibition of acetoclastic methanogenesis in granular sludge, Appl. Microbiol. Biotechnol., 34 (4), 544–550.
  • Sikkema J., de Bont J.A.M., Poolman B., 1995, Mechanisms of membrane toxicity of hydrocarbons, Microbiol. Rev., 59 (2), 201–222.
  • Speransky V.V., Speransky S.R., Brawley S.H., 1999, Cryoanalytical studies of freezing damage and recovery in Fucus vesiculosus (Phaeophyceae), J. Phycol., 35, 1264–1275.
  • Sterman N.T., 1988, Spectrophotometric and fluorometric chlorophyll analysis, [in:] Experimental phycology: a laboratory manual, C. S. Lobban, D. J. Chapman & B.P. Kremer (eds.), Cambridge Univ. Press, New York, 35–46.
  • Sullivan M. J., Currin C.A., 2000, Community structure and functional dynamics of benthic microalgae in salt marshes, [in:] Concepts and controversies in tidal marsh ecology, M.P. Weinstein & D.A. Kreeger (eds.), Kluwer Acad. Publ., Dordrecht, 81–106.
  • Tien C. J., 2004, Some aspects of water quality in a polluted lowland river in relation to the intercellular chemical levels in planktonic and epilithic diatoms, Water Res., 38 (7), 1779–1790.
  • Tongpim S., Pickard M.A., 1996, Growth of Rhodococcus S1 on anthracene, Can. J. Microbiol., 42 (3), 289–294.
  • Torzillo G., 1998, Optimization of microalgal productivity outdoors: use of an online chlorophyll fluorescence technique, 52nd Ann. Meet. Phycol. Soc. Am.
  • Veron B., Dauguet J. C., Billard C., 1998, Sterolic biomarkers in marine phytoplankton. II. Free and conjugated sterols of seven species used in mariculture, J. Phycol., 34, 273–279.
  • Volkering F., Breure A.M., Sterkenburg A., van Andel J.G., 1992, Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics, Appl. Microbiol. Biot., 36, 548–552.
  • Wang Y., Tang X., Li Y., Liu Y., 2002, Stimulation effects of anthracene on marine microalgae growth, Sci. Total Environ., 13 (3), 343–346.
  • Whyte L.G., Hawari J., Zhou E., Bourbonni´ere L., Inniss W. E., Greer C.W., 1998, Biodegradation of variable-chain length alkanes at low temperatures by a psychrotrophic Rhodococcus sp., Appl. Environ. Microbiol., 64 (7), 2578–2584.
  • Wodziński R. S., Coyle J.E., 1974, Physical state of phenanthrene for utilization by bacteria, Appl. Micobiol., 27 (6), 1081–1084.
  • Yang S., Wu R. S., Kong R.Y., 2002, Physiological and cytological responses of the marine diatom Skeletonema costatum to 2, 4-dichlorophenol, Aquat. Toxicol., 60 (1)–(2), 33–41.
  • Zittelli G. C., Lavista F., Bastianini A., Rodolfi L., Vincenzini M., Tredici M. R., 1999, Production of eicosapentaenoic acid by Nannochloropsis sp. cultures in outdoor tubular photobioreactors, J. Biotechnol., 70, 299–312.

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Bibliografia

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