UDP-N-acetylglucosaminc can be bound by pure ribosomes. The part of N-acetylglucosamine-1-P can be transferred from the complex ribosome-UDP-N-acetylglucosamine onto dolichol phosphate. Evidence Is presented that N-acetylglucosamine bound to dolichol phosphate can be transferred to the nascent peptide synthesized on the ribosomc.
1. Kopacz-Jodczyk. T.. Paszkiewicz-Gadek, A. & Gałasiński. W. (1988) llie altachcment of IJDP-hexosamine to die ribosomes isolated from rai liver. Biochem. Med. Mctab. Biol., 39, 290 - 295.
2. Gałasiński. W. & Moldave, K. (1969) Purification of aminoacyltransferasc II (translocation factor) from rat liver. J. Biol. Chem.. 244.6527 - 6532.
3. Jabłonowska. K.. Kopacz-Jodczyk. T.. Nied/wiecka. J. & Gałasiński. W. (1983) Isolation and characterization of elongation factor EF-2 from Guerin tumour. Acta Biochim. Polon., 30, 381 - 389.
4. Moldave, K. & Skogcnson, L. (1967) Purification of mammalian ribosomes Methods Enzymoi, 12A. 478 - 480.
5. Skogerson, G. & Moldave. K. (1967) llic binding of aminoacyl transferase II to ribosomes. Biochem. Biophys. Res. Cornmun., 27,568 - 572.
6. Peiermann, M. L. (1964) The physical and chemical properties of ribosomes, p. 63. American Elsevier, New York.
7. Lowry. O. II.. Kosebrough. N. J., Fan; A. L. & Randall. R. J. (1951) Protein measurement witli the Folin phenol reagent. J. Biol. Chem., 193, 265 275.
8. Vijay, I. K., Perdev. G. H. & Lewis, D. E. (1980) Biosynthesis of mammary glycoproteins. Partial characterization of die sequence for llie assembly of lipid-Iinked saccharides. J. Biol. Chem., 255, 11210- 11220.
9. Shailubhai, K., Dong-Yu, B., Saxena, E. S. & Vijay, I. K. (1988) Purification and characterization of UDP-/V-acetyl-L>-glucosaminc: dolichol phosphate /V-acctyl-r>- glucosamine-1 -phosphate transferase involved in the biosynthesis of asparginc-linked glycoproteins in die mammary gland. J. Biol. Chem., 263. 15964 - 15972.
10. Kaushal, G. P. & Elbein, A. D. (1985) Purificaiion and properties of IJDP-GlcNac: I)olicliyl-phosphate GlcNac-1 -phosphate transferase. Activation and inhibition of die enzyme. J. Biol. Chem.. 260.16303 - 16309.
11. Palamarczyk, G. & Hemming, R. W. (1975) Hie formation of mono-iV-acelyl- hexosaminc derivatives of dolichol diphosphate by pig liver microsomal fraction. Biochem. J., 148. 245 - 251.
12. Folch. J., I.ees, M. & Sloane. S. G. II. (1957) A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226.497 - 509.
13. Leder, P. & Uursztyn. J1. (1966) Initiation of protein synthesis. II. A convenient assay for the ribosome dependent synthesis of /V-formyl ,4C-metionylpuromycin. Biochem. Biophys. Res. Com/ruin., 25, 233 - 238.
14. Kyossev, Z. & Ossikovski, F.. (1991) Biosynthesis of dolichyl diphospliate N-ace- tylglucosaminc intermediates in Ascaridia galli microsomes. Int. J. Biochem., 23, 749 - 753.
15. Palamarczyk, G, Lehle. L., Marikowski, T., Chojnacki, T. & lanner. W (1980) Specificity of solubilized yeast glycosyl transferases for polyprenyl derivatives. Eur. J. Biochem. 105, 517 - 523.
16. Trcvelyan, W. E., Procter, D. K. & 1 larrison, J. S. (1950) Detection of sugars on paper chromatogram. Nature (London). 166.444 - 445.
17. Villemez.C. L. V. & Carlo. P. L. (1980) Properties of a soluble polyprenyl phosphate. UDP-D-Af-acetylglucosamine A/-acelylglucosamine-l-phosphate transferase. J.Biol. Chem., 255,8174-8178.