Influence of methisoprinol on the replication of rhabdoviruses isolated from carp [Cyprinus carpio] and catfish [Ictalurus melas]: in vitro study

• Autorzy: Siwicki A. K. , Pozet F. , Morand M. , Kazun B. , Trapkowska S. , Malaczewska J.
• Języki publikacji: EN

Abstrakty

EN

Rhabdoviruses constitute one of the most pathogenic viruses isolated from: rainbow trout and carp culture. Several viruses were also isolated from other species of fish. These viruses are mostly "associated with epizootics and heavy losses. Spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV) have been the most extensively studied, due to their significant economic impact. Significant progress has been made towards controlling the major bacterial fish diseases using vaccines, but this approach has not yet been successful in preventing viral diseases in fish culture. However, for an effective therapeutic approach, specific drugs should be developed to selectively inhibit virus replication and/or stimulate antiviral protection. In this investigation we examined the in vitro influence of methisoprinol on the SVCV and virus isolated from catfish (Ictalurus melas) replication by measuring their RNA synthesis. The viruses were propagated in EPC cells and cell cultures containing methisoprinol were followed by infection with SVCV or catfish rhabdovirus suspension containing 107TCID50/ml. Methisoprinol (Polfa, Poland) at concentrations of 0, 100, 200, 300, 400 and 500 μg/ml of medium (Glasgow MEM) was used in this study. The results of this study show the strong inhibition of incorporation (cpm) of [3H]-uridine into SVCV and catfish rhabdovirus RNA in cell culture exposed to methisoprinol at various concentrations. The highest percent of inhibition of viral RNA at 72 h after infection with two rhabdoviruses were observed in doses of 400 and 500 μg/ml of methisoprinol in medium. The results of this in vitro study showed that methisoprinol inhibits the rhabdoviruses isolated from carp and catfish.

Słowa kluczowe

EN

catfish; carp; fish; rhabdovirus; replication; Ictalurus melas; in vitro; animal disease; carp culture; Cyprinus carpio; veterinary medicine; virus; methisoprinol; rainbow trout

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2003
• Tom: 06
• Numer: 1
• Opis fizyczny: p.47-50,fig.

Twórcy

autor

Siwicki A. K.

• University of Warmia and Mazury, Oczapowskiego 13, Olsztyn, Poland

autor

Pozet F.

autor

Morand M.

autor

Kazun B.

autor

Trapkowska S.

autor

Malaczewska J.