Effect of a preparation containing lactic fermentation bacteria on the hygienic status and aerobic stability of silages

• Autorzy: Selwet M.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.

Słowa kluczowe

EN

silage; aerobic stability; hygienic status; biological additive; preparation; lactic fermentation bacteria; bacteria; nutritive value; hygienic quality; grass-legume silage

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2008
• Tom: 11
• Numer: 1
• Opis fizyczny: p.35-39,ref.

Twórcy

autor

Selwet M.

• August Cieszkowski Agricultural University in Poznan, 60-656 Poznan, Poland

Bibliografia

• AOAC (1990) Official Methods of Analysis, 15th ed., Washington, DC.
• Aksu T, Baytok E, Bolat D (2004) Effects of bacterial in- oculant on corn silage fermentation and nutrient digesti­bility. Small Ruminat Res 55:249-252.
• Conway EJ, (1962) Microdiffusion analysis and volumetric error, 5th ed., Crosby Lokwood & Son Ltd, London.
• Deibel RH, Evans JB, Niven CF (1957) Microbiological as­say for the thiamin using Lactobacillus viridescsens. J Bac­terid 74: 818-821.
• Driehuis F, Oude Elfering S (2000) The impact of the qual­ity of silage on animal health and food safety: a review. Vet Quart 22: 212-216.
• Filya I (2004) Nutritive value and aerobic stability of whole crop maize silage harvested at four stages of maturity. Anim Feed Sci Tech 116: 141-150.
• Filya I, Sucu E, Karabulut A (2006) The effects of Propionibacterium acidipropionici and Lactobacillus plan- tarum, applied at ensiling, on the fermentation and aero­bic stability of low dry matter corn and sorghum silages. J Ind Microbiol Biot 33: 353-358.
• Giffel MC, Wagendorp A, Herrewegh A, Driehuis F (2002) Bacterial spores in silage and raw milk. Anton Leeuw Int 81: 625-630.
• Grajewski J, Potkański A, Raczkowska-Werwinska K, Twarożek M, Miklaszewska B, Grabowska M, Gubata A, Selwet M (2007) Hygienic quality of horn silage with a biological and chemical additive. Med Weter 63: 205-208.
• Kleinschmit DH, Schmidt RJ, Rung L Jr (2005) The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J Dairy Sci 88: 2130-2139.
• Rung L Jr, Myers CL, Neylon JM, Taylor CC, Lazartic J, Mills JA, Whiter AG (2004) The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of high moist­ure corn and whole-crop barley. J Dairy Sci 87: 1310-1316.
• Manafi M, Rneifel W (1989) A combined chromogenic-fluorogenic medium for simultaneous de­tection of total coli form and E. coli in the water. Zen- tralbl Hyg 189: 225-234.
• Me Donald P, Henderson AR (1964) Determination of water-soluble carbohydrates in grass. J Sci Food Agri 15: 395-398.
• Muck RE, Filya I, Contreras-Govea FE (2007) Inoculant effects on alfalfa silage in vitro gas and volatile fatty acid production. J Dairy Sci 90: 5115-5125.
• Orthy DS (1977) Comparison of sulfite-polymyxin-sul­fadiazine medium and tryptose-sulfite-cycloserine me­dium without eggyolk for recovering Clostridium peifrin- gens. Appl Environ Microb 33: 986-988.
• Rundberget T, Skaar I, Floapyen A (2004) The presence of Pénicillium and Pénicillium mycotoxins in food wastes. Int J Food Microb 90: 181-188.
• SAS (1999) SAS/STAT User Guide (Release 6.03) SAS In­stitute Inc Cary NC.
• Selwet M (2004) Influence of formic acid on bacterial growth during the ensilage of grass-legume mixtures. Med Weter 60: 763-765.
• Selwet M (2005) Effect of preservatives based on formic acid on the development of yeasts and mold fungi in si­lages. Med Weter 61: 349-352.
• Selwet M (2006) Effect of organic acids and bacterial-enzy­matic preparations on number of fungal populations and silage aerobic stability. Bull Vet Inst Puławy 50:215-220.
• Steidlova S, Kalać P (2002) Levels of biogenic amines in maize silages. Anim Feed Sci Tech 102: 197-205.
• Wohlt JE (1989) Use of silage inoculant to improve feeding stability and intake of a corn silage-grain diet. J Dairy Sci 72: 545-551.