Measurement of protozoan grazing on bacteria by means of [3H-thymidine]-labeled natural assemblages of lake bacteria

• Autorzy: Koton-Czarnecka M , Chrost R.J.
• Języki publikacji: EN

Abstrakty

EN

Protozoan grazing on bacteria transfers significant amounts of organic carbon to higher trophic levels in aquatic ecosystems. Therefore, the determination of protozoan grazing rates on bacteria is necessary for estimating the efficiency of microbial loops in organic matter utilization and transformation. Currently, a variety of methods are available to measure protozoan grazing on bacteria but most of them result in serious physical perturbations of the examined samples. We describe an assay for protozoan grazing on bacteria by means of [3H-methyl]thymidine-labelled natural bacterial assemblages as a food tracer for protozoans. Using this method we determined the protozoan grazing rates on bacteria in ten Mazurian lakes in April and July, 2000. We compared the grazing rates with bacterial biomass production and the trophic state index of studied lakes.

Słowa kluczowe

EN

lake; measurement; trophic status; aquatic ecosystem; microbial loop; organic carbon; bacteria; H-radiolabelled bacteria; biomass production; protozoan grazing

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2002
• Tom: 11
• Numer: 4
• Opis fizyczny: p.385-393,fig.

Twórcy

autor

Koton-Czarnecka M

• University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland

autor

Chrost R.J.

Bibliografia

• 1. BERNINGER U.G., FINDLAY B.J., KUUPPO-LEINIKKI P. Protozoan control of bacterial abundances in freshwater. Limnol. Oceanogr. 36, 147, 1991.
• 2. SANDERS R.W., PORTER KG., BENNETT S.L., DE­BIASE A.E. Seasonal patterns of bacterivory by flagellates, ciliates, rotifers, and cladocerans in a freshwater planktonic community. Limnol. Oceanogr. 34, 673, 1989.
• 3. WEISSE T., MULLER H. Planktonic protozoa and the microbial food web in Lake Constance. Arch. Hydrobiol. Spec. Issues. Advanc. Limnol. 53, 223, 1998.
• 4. LAUTERBORN R. Zur Kenntnis des Planktons des Boden­sees und der benachbarten Kleiseen. Mitteilungen des Badischen Landesvereins fur Natyrkunde und Naturschutz. Neue Folge Bd.1, 421, 1925.
• 5. AZAM F., FENCHEL T, FIELD J.G, GRAY J.S, MEYER-REIL A., THINGSTAD F. The ecological role of watercolumn microbes in the sea. Mar. Ecol. Prog. Ser. 10, 257, 1983.
• 6. McMANUS G.B., FUHRMAN J.A. Bacterivory in seawater studied with the use of inert fluorescent particles. Limnol. Oceanogr. 31, 420, 1986.
• 7. SHERR B.F, SHERR E.B., FALLON R.D. Use of mono- dispersed, fluorescently labeled bacteria to estimate in situ protozoan bacterivory. Appl. Environ. Microbiol. 53, 958, 1987.
• 8. LANDRY M.R., HASSET R.P. Estimating the grazing im pact of marine micro-zooplankton. Mar. Biol. 67, 283, 1982.
• 9. KUUPPO-LEINIKKI P., KUOSA H. Estimation of flagel late grazing on bacteria by size fractionation in the Northern Baltic Sea. Arch. Hydrobiol. Beih. 34, 283, 1990.
• 10. SHERR B.F., SHERR E.B., ANDREW T.L., FALLON R.D., NEWELL S.Y. Trophic interactions between hetero­trophic Protozoa and bacterioplankton in estuarine water analysed with selective metabolic inhibitors. Mar. Ecol. Prog. Ser. 32,169, 1986.
• 11. HOLLIBAUGH J.T., FUHRMAN J.A., AZAM F. Radioactively labeling of natural assembleges of bacterioplankton for use in trophic studies. Limnol. Oceanogr. 25, 172, 1981.
• 12. NYGAARD K., HESSEN D.O. Use of 14C-protein-labelled bacteria for estimating clearance rates by heterotrophic and mixotrophic flagellates. Mar. Ecol. Prog. Ser. 68, 7, 1990.
• 13. JAR VIS A.C., HART R.C. Determining in situ bacterial fil tration rates by zooplankton: improvements to the [methyl- 3H]thymidine labelling technique. J. Plankton Res. 15, 277, 1993.
• 14. ROMAN M.R., RUBLEE P.A. A method to determine in situ zooplankton grazing rates on natural particle assem blages. Mar. Biol. 65, 303, 1981.
• 15. FORSYTH D.T., JAMES M.R. Zooplankton grazing on lake bacterioplankton and phytoplankton. J. Plankton Res. 6, 803, 1984.
• 16. SERVAIS P., BILLEN G., REGO J.V. Rate of bacterial mortality in aquatic environments. Appl. Environ. Microbiol. 49, 1448, 1985.
• 17. BJORNSEN P.K., LARSEN J.P., GEERTZ-HANSEN O., OLESEN M. A field technique for the determination of zo oplankton grazing on natural bacterioplankton. Freshwater Biol. 17, 151, 1986.
• 18. BERN L. Zooplankton grazing on [methyl-3H]thymidine-labelled natural particles assemblages: determination of filtering rates and food selectivity. Freshwater Biol. 17, 151, 1987.
• 19. SERVAIS P., MARTINEZ J, BILLEN G., VIVAS-REGO J. Determining (3H)thymidine incorporation into bacteriop lankton DNA: improvement of the method by Dnase treat ment. Appl. Environ. Microbiol. 53, 1977, 1987.
• 20. FUHRMAN J.A., AZAM F. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar. Biol. 66, 109, 1982.
• 21. LANDRY M.R., HAAS L.W, FAGERNESS V.L. Dyna mics of microbial plankton communities: experiments in Kaneohe Bay, Hawaii. Mar. Ecol. Prog. Ser. 16, 127, 1984.
• 22. SANDERS R.W., PORTER K.G. Use of metabolic inhibi tors to estimate protozooplankton grazing and bacterial production in a monomictic eutrophic lake with an anaerobic hypolimnion. Appl. Environ. Microbiol. 52, 101, 1986.
• 23. PORTER K. G., FEIG Y. S. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25, 943, 1980.
• 24. CHROST R. J., OVERBECK J., WCISLO R. Evaluation of the [3H]thymidine method for estimating bacterial growth rates and productionnin the lake water: re-examination and methodological comments. Acta Microbiol. Polon. 37, 95, 1988.
• 25. CHROST R. J., RAI H. Bacterial secondary production. Microbial Ecology of Lake PluBee, Springer-Verlag, New York, pp 92-117, 1994.
• 26. LEE S., FUHRMAN J. A. Relationships between biovolume and biomass of naturally derived marine bacterioplankton. Appl. Environ. Microbiol. 53, 1298, 1987.
• 27. STRICLAND J.D.H., PARSONS T.R. A practical hand book of seawater analyses. 2nd edn. Bull. Fish. Res. Bd Can. 167, 1972.
• 28. CARLSON R.E., A trophic state index for lakes. Limnol. Oceanogr. 22, 361, 1977.
• 29. WIKNER J., RASSOULZADEGAN F., HAGSTROM A. Periodic bacterivore activity balances bacterial growth in the marine environment. Limnol. Oceanogr. 35, 313, 1990.
• 30. GUDE H. Influence of phagotrophic processes on the re generation of nutrients in two-stage continuous culture systems. Microb. Ecol. 11, 193, 1985.
• 31. GUDE H. The role of grazing on bacteria in plankton succession, (in:) Plankton Ecology: Succession in Plankton Communities., red. Sommer U., Brock/Springer, Berlin, pp: 337-364, 1989.
• 32. SIMEK K., VRBA J., PERNTALER J., POSCH T., HART­MAN P, NEDOMA J, PSENNER R. Morphological and composition shifts in an experimental bacterial community influenced by protists with contrasting feeding modes. Appl. Environ. Microbiol. 63, 587, 1997.
• 33. DEL GIORGIO P.A., GASOL J.M., VAQUE D., MURA P., AGUSTI S., DUARTE CM. Bacterioplankton community structure: protists control net production and the pro portion of active bacteria in a coastal marine community. Limnol. Oceanogr. 41, 1169, 1996.
• 34. KUUPPO-LEINIKKI P. Protozoan grazing on planktonic bacteria and its impact on bacterial population. Mar. Ecol. Prog. Ser. 63, 227, 1990.
• 35. KOTON-CZARNECKA M., CHROST R.J. Konsumpcja bakterii przez pierwotniaki w ekosystemach wodnych. Post. Mikrobiol. 40, 219, 2001.
• 36. DISALVO L.H. Regenerative functions and microbial ecol ogy of coral reefs: ingestion of labelled bacteria in a coral reef microcosm. J. Exp. Mar. Biol. Ecol. 7, 123, 1971.
• 37. KING K.R., HOLLIBAUGH J.T., AZAM F. Predator-prey interactions between the larvacean Oikopleura dioica and bacterioplankton in enclosed water columns. Mar. Biol. 56, 49, 1980.
• 38. LESSARD E.J., SWIFT E. Species-specific grazing rates of heterotrophic dinoflagellates in oceanic waters, measured with dual-label radioisotope technique. Mar. Biol. 87, 289, 1985.
• 39. DUCKLOW H.W., PURDIE D.A., WILIAMS P.J.B., DA VIES J.M. Bacterioplankton: a sink for carbon in a coastal marine plankton community. Science 232, 865, 1986.
• 40. CARON D.A., LESSARD E. J., VOYTEK M., DENNETT M.R. Use of tritiated thymidine (TdR) to estimate rates of bacterivory: implications of label retention and release by bacterivores. Mar. Microb. Food Webs 7, 177, 1993.
• 41. ROB ARTS R.D., ZOHARY T. Fact or fiction - bacterial growth rates an production as determined by [3H-methyl]thymidine? Adv. Microb. Ecol. 13, 121, 1993.
• 42. HANSEN B., HANSEN P.J., BJORSEN P.K. The size ratio between planktonic predators and their prey. Limnol. Oceanogr. 39, 395, 1994.
• 43. CHROST J.R., KOTON M., SIUDA W. Bacterial secondary production and bacterial biomass in four Mazurian Lakes of differing trophic status. Polish J. Environ. Studies 9, 255, 2000
• 44. BEAVER J.R., CRISMAN T.L. the role of ciliated protozoa in pelagic freshwater ecosystems. Microb. Ecol. 17, 111, 1989.
• 45. FUKAMI K, MEIER B., OVERBECK J. Vertical and temporal changes in bacterial production and its consumption by heterotrophic nanoflagellates in a north German eutrophic lake. Arch. Hydrobiol. 122, 129, 1991.
• 46. NAGATA T. The microflagellate - picoplankton food link­age in the water column of Lake Biwa. Limnol. Oceanogr, 33, 504, 1988.
• 47. PERNTHALER J., SIMEK K., SATTLER B., SCHWAR­ZENBACHER A, BOBKOVA J, PSENNER R. Short-term changes of protozoan control on autotrophic picoplankton in an oligo-mesotrophic lake. J. Plankton Res. 38, 443, 1996.
• 48. WEISSES T. The annual cycle of heterotrophic freshwater nanoflagellates: role of bottom-up versus top-down control. J. Plankton Res. 13, 167, 1991.
• 49. WIELTSCHNIG C, WIHLIDAL P., ULBRICHT T., KIR­SCHNER A.K.T., VELIMIROV B. Low control of bacterial production by heterotrophic nanoflagellates in a eutrophic backwater environment. Aquat. Microb. Ecol. 17, 77, 1999.
• 50. CHRISTOFFERSEN K., RIEMANN B., KLYSNER A., SONDERGAARD M. Potential role of fish predation and natural populations of zooplankton in structuring a plankton community in eutrophic lake water. Limnol. Oceanogr. 38, 561, 1993.
• 51. DOLAN J.R., GALLEGOS C.L. Trophic coupling of rotifers, microflagellates, and bacteria during fall months in the Rhode River Estuary. Mar. Ecol. Prog. Ser. 77, 147, 1991.
• 52. JtlRGENS K., GUDE H. The potential importance of grazing-resistant bacteria in planktonic systems. Mar. Ecol. Prog. Ser. 112, 169, 1994.
• 53. GONZALES J.M., SUTTLE C.A. Grazing by marine nanof lagellates on viruses and virus-sized particles: ingestion and digestion. Mar. Ecol. Prog. Ser. 94, 1, 1993.
• 54. VAN WAMBEKE F. Influence of phytoplankton lysis or grazing on bacterial metabolism and trophic relationships. Microb. Ecol. 27, 143, 1994.
• 55. SHERR E.B. Direct use of high molecular weight polysac charide by heterotrophic flagellates. Nature 335, 348, 1988.
• 56. SANDERS R.W., CARON D.A., BERNINGER U.G. Rela tionship between bacteria and heterotrophic nanoplankton in marine and fresh waters: an inter-ecosystem comparison. Mar. Ecol. Prog. Ser. 86, 1, 1992.