Limitation of usage of PicoGreen dye in quantitative assays of double-stranded DNA in the presence of intercalating compounds

• Autorzy: Koba M. , Szostek A. , Konopa J.
• Języki publikacji: EN

Abstrakty

EN

PicoGreen is a very sensitive fluorescent dye for quantitative assays of double-stranded DNA (dsDNA) in solution and is used in several analytical protocols in which sensitive and precise DNA detection is needed, also for examination of drug–DNA interactions. The data shown in this paper indicate that compounds intercalating to DNA influence the applicability of PicoGreen dye for quantitative measurements of dsDNA, and for this reason PicoGreen dye is not suitable for examination of drug–DNA interactions, especially interstrand DNA crosslinks.

Słowa kluczowe

PL

PicoGreen dye; quantitative assay; double stranded DNA; drug-DNA binding

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 4
• Opis fizyczny: p.883-886,fig.,ref.

Twórcy

autor

Koba M.

• Collegium Medicum of Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland

autor

Szostek A.

autor

Konopa J.

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