Influence of organic solvents on papain kinetics

• Autorzy: Szabelski M. , Stachowiak K. , Wiczk W.
• Języki publikacji: EN

Abstrakty

EN

Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. More­over, the number of papain active sites decreased with the increase of DMF and MeOH concentration.

Słowa kluczowe

EN

fluorogenic substrate; organic solvent; enzyme activity; papain; papain kinetics

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 4
• Opis fizyczny: p.1197-1201,fig.

Twórcy

autor

Szabelski M.

• University of Gdansk, J.Sobieskigo 18, 80-952 Gdansk, Poland

autor

Stachowiak K.

autor

Wiczk W.

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