Beta-laktamazy o rozszerzonym spektrum substratowym u Gram-ujemnych paleczek rosnacych w warunkach bezwzglednie beztlenowych

• Autorzy: Rokosz A. , Sawicka-Grzelak A. , Kot K. , Pituch H. , Meisel-Mikolajczyk F. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Przedmiotem badań było 100 klinicznych szczepów pałeczek Gram-ujemnych, należących do bezwzględnych beztlenowców (93 szczepy z rodzaju Bacteroides i 7 szczepów z rodzaju Prevotella). Stwierdzono, że znaczna większość szczepów ((95%) wykazuje wrażliwość na piperacylinę, piperacylinę z tazobaktamem, tikarcylinę z kwasem klawulanowym, imipenem i meropenem. Wykryto dwa szczepy gatunku Bacteroides fragilis, wytwarzające ß-laktamazy o rozszerzonym zakresie działania (ESBLs). U badanych szczepów nie znaleziono indukowanych ß-laktamaz (IBL-s).

EN

This study was performed to determine the susceptibility of the clinical strains of Gram-negative strictly anaerobic rods to newer ß-lactam antibiotics. Also, the trial was undertaken to detect strains producing extended-spectrum ß-lactamases (ESBLs) and inducible ß-lactamases (IBLs) among Bacteroides spp. and Prevotella spp. rods isolated from hospitalized patients. One hundred strains of Gram-negative, obligatory anaerobic rods were applied in the study. The strains were identified in automatic ATB system using API 20 A strips. ß-lactamase-positive strains were determined with disc nitrocefin test. ESBL-producing strains were detected with double disc test according to Jarlier et al. (1988). Clavulanate was applied as the inhibitor of these ß-lactamases (AMO/CLAV disc). ESBL-positive strains were confirmed with the use of E test (TZ/TZL strip). Inducible ß-lactamases were determined by double disc method according to Sanders and Sanders (1979). Cefoxitin was the inducer of these ß-lactamases (FOX disc). Among 93 Bacteroides spp. strains and 7 Prevotella spp. strains, 91 strains (91%) produced ß-lactamases. Two ESBL-producing strains (2%) were detected. Strains producing inducible ß-lactamases (IBL) were not found. A high activity of the examined ß-!actam antibiotics against strains of Gram-negative anaerobes was found. The majority of strains were susceptible to piperacillin (95%), piperacillin combined with tazobactam (99%), ticarcillin combined with clavulanic acid (99%), meropenem (97%) and imipenem (99%). The obtained results indicate the necessity of ESBL determination among strains of the genus Bacteroides, isolated from clinical specimens. Newer ß-lactam antibiotics, especially penicillins in combination with ß-lactamase inhibitors and carbapenems, are useful in empiric therapy of infections caused by Bacteroides spp. and Prevotella spp. anaerobic rods.

Słowa kluczowe

PL

Prevotella; infekcja bakteryjna; Bacteroides; szczepy bakteryjne; antybiotyki; beta-laktamaza; leczenie; Fusobacterium; Porphyromonas; wrazliwosc na antybiotyki; Clostridium

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2000
• Tom: 52
• Numer: 2
• Opis fizyczny: s.129-137,tab.,bibliogr.

Twórcy

autor

Rokosz A.

• Akademia Medyczna, 02-004 Warszawa, ul.T.Chalubinskiego 5

autor

Sawicka-Grzelak A.

autor

Kot K.

autor

Pituch H.

autor

Meisel-Mikolajczyk F.

autor

Luczak M.

Bibliografia

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