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Tytuł artykułu

Cytological assessment of carrot plants obtained in anther culture

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Anthers of Feria F1 and Narbonne F1 carrot cultivars were cultured in vitro to induce androgenic embryos. To confirm the microspore origin of developed embryos, chromosome counts of root tip meristematic cells were made for each carrot plant obtained in anther culture. Using phase contrast technique and fluorescence microscopy, cytological changes of microspores during culture leading to proembryo formation were documented in the first days after anther placement on the induction medium. More than 90% of the carrot plants obtained in anther cultures had no haploid chromosomes.

Wydawca

-

Rocznik

Tom

50

Numer

2

Opis fizyczny

p.7-11,fig.,ref.

Twórcy

autor
  • Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
autor
autor
autor

Bibliografia

  • Andersen SB, Christiansen J, and Farestveit B. 1990. Carrot (Daucus carota L.): in vitro production of haploids and field trials. In: Bajaj YPS [ed.], Biotechnology in agriculture and forestry, 393-402. Springer-Verlag, Berlin, Heidelberg.
  • Gamborg OL, Miller RA, and Ojima O. 1968. Nutrient requirements of suspension cultures of soybean root cell. Experimental Cell Research 50: 151-158.
  • Górecka K. 1998. Obtaining of homozygous lines of head cabbage (Brassica oleracea L. var. capitata L.) with aid of anther cultures. Habilitation thesis, 14. Research Institute of Vegetable Crops Skierniewice, Poland. (In Polish with English summary).
  • Górecka K, Krzyżanowska D, and Górecki R. 2005a. The influence of several factors on the efficiency of androgenesis in carrot. Journal of Applied Genetics 46: 265-269.
  • Górecka K, Krzyżanowska D, Kiszczak W, and Górecki R. 2005b. Embryo induction in anther culture of Daucus carota L. Vegetable Crops Research Bulletin 63: 25-32.
  • Keller WA, Rajhathy R, and Lacapra J. 1975. In vitro production of plants from pollen in Brassica campestris. Canadian Journal of Genetics and Cytology 17: 655-666.
  • Kiszczak W, Górecka K, Krzyżanowska D, and Kowalska U. 2005. Size of flower buds in carrot (Daucus carota L.) as an indicator of a stage of microsporogenesis and its suitability for induction of androgenesis. Vegetable Crops Research Bulletin 62: 81-90.
  • Krzyżanowska D, and Górecka K. 2004. The effect of the some factors on embryogenesis in anther culture of Brussels sprouts. Vegetable Crops Research Bulletin 60: 17-23.
  • Maraschin SF, de Priester W, Spaink HP, and Wang M. 2005. Androgenic switch: an example of plant androgenesis from the male gametophyte perspective. Journal of Experimental Botany 56: 1711-1726.
  • Matthys-Rochon E. 2005. Secreted molecules and their role in embryo formation in plants: a mini-review. Acta Biologica Cracoviensia Series Botanica 47/1: 23-29.
  • Nitsch C, and Norreel B. 1973. Histophysiologie végétable, effect d’un choc thermique sur le pouvoir embryogéne du pollen de Datura innoxia cultivé dan l'anthére ou isolée de l'anthére. Comptes Rendus de l'Académie des Sciences, D. 276: 303-306.
  • Pauls KP, Chan J, Woronuk G, Schulze D, and Brazolot J. 2006. When microspores decide to become embryos - cellular and molecular changes. Canadian Journal of Genetics and Cytology 84: 668-678.
  • Tyukavin GB, Shmykova NA, and Monakhova MA. 1999. Cytological study of embryogenesis in cultured carrot anthers. Fiziologiya Rastenii 46: 876-884.
  • Wang M, van Bergen S, and van Duijn B. 2000. Update on plant development. Insights into a key developmental switch and its importance for efficient plant breeding. Plant Physiology 124: 523-530.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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