Involvement of the essential yeast DNA polymerases in induced gene conversion

• Autorzy: Halas A. , Ciesielski A. , Zuk J.
• Języki publikacji: EN

Abstrakty

EN

In the yeast Saccharomyces cerevisiae three different DNA polymerases α, δ and ε are involved in DNA replication. DNA polymerase α is responsible for initiation of DNA synthesis and polymerases δ and ε are required for elongation of DNA strand during replication. DNA polymerases δ and ε are also involved in DNA repair. In this work we studied the role of these three DNA polymerases in the process of recombinational synthesis. Using thermo-sensitive heteroallelic mutants in genes encoding DNA polymerases we studied their role in the process of induced gene conversion. Mutant strains were treated with mutagens, incubated under permissive or restrictive conditions and the numbers of convertants obtained were compared. A very high difference in the number of convertants between restrictive and permissive conditions was observed for polymerases α and δ, which suggests that these two polymerases play an important role in DNA synthesis during mitotic gene conversion. Marginal dependence of gene conversion on the activity of polymerase ε indicates that this DNA polymerase may be involved in this process but rather as an auxiliary enzyme.

Słowa kluczowe

EN

yeast; DNA polymerase; gene conversion; DNA replication; DNA synthesis; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.863-872,fig.

Twórcy

autor

Halas A.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warszawa, Poland

autor

Ciesielski A.

autor

Zuk J.

Bibliografia

• Araki, H., Ropp, P.A., Johnson, A.L., Johnson, L.H., Morrison, A. & Sugino, A. (1992) DNA polymerase II, the probable homolog of mam­malian DNA polymerase e, replicates chromo­somal DNA in the yeast Saccharomyces cerevisiae. EMBO J. 11, 733-740.
• Averbeck, D. (1994) Photoaddition by furo- coumarins; in Photobiology in Medicine (Jori et al, ed.) pp. 71-S8, Plenum Press, New York.
• Bauer, G.A., Heller, H.M. & Burgers, P.M.J. (1988) DNA polymerase III from Saccharomyces cerevisiae. J. Biol Chem. 263, 917-924.
• Blank, A., Kim, B. & Loeb, L. (1994) DNA polymer­ase ó is required for base-excision repair of DNA methylation damage in Saccharomyces cerevisiae. Proc. Natl Acad. Sci U.S.A. 91, 9047-9051.
• Boulet, A., Simon, M., Faye, G., Bauer, G.A. & Bur­gers, P.M.J. (1989) Structure and function of the Saccharomyces cerevisiae CDC2 gene en­coding the large subunit of DNA polymerase III. EMBO J. 8, 1849-1854.
• Budd, M.E., Wittrupt, K.D., Bailey, J.E. & Camp­bell, J.L. (1989) DNA polymerase I is required for premeiotic DNA replication and sporu- lation but not for X-ray repair in Saccharo­myces cerevisiae. Mol Cell Biol 9, 365-376.
• Budd, M.E. & Campbell, J.L. (1993) DNA polymerases <5 and e are required for chromo­somal replication in Saccharomyces cerevisiae. Mol. Cell. Biol 13, 496-505.
• Budd, M.E. & Campbell, J.L. (1995) DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae. Mol CellL Biol 15, 2173-2179.
• Budd, M.E. & Campbell, J.L. (1997) The roles of the eucaryotic DNA polymerases in DNA re­pair synthesis. Mutat Res. 384, 157-167.
• Chan, G.L., Doetsch, P.W. & Haseltine, W.A. (1985) Cyclobutane pyrimidine dimers and (6-4) photoproducts block polymerisation by DNA polymerase I. Biochemistry 24, 5723- 5728.
• Fabre, F., Boulet, A. & Faye, G. (1991) Possible in­volvement of the yeast POLIII DNA polymer­ase in induced gene conversion. Mol Gen. Genet 229, 353-356.
• Foiani, M., Martini, F., Gamba, D., Lucchini, G. & Plevani, P. (1994) The B subunit of the DNA polymerase a-primase complex in Saccharo­myces cerevisiae executes an essential function at the initial stage of DNA replication. Mol Cell Biol 14, 923-933.
• Ilalas, A., Barauoweka, H., Policinska, Z. & Jachymczyk, W.J. (1997) Involvement of the REV3 gene in the methylated base-excision re­pair system. Co-operation of two DNA polymerases, d and Rev3p, in the repair of MMS-induced lesions in the DNA of Sac­charomyces cerevisiae. Curr. Genet. 31, 292- 301.
• Halas, A., Policinska, Z., Baranowska, H. & Jachymczyk, W.J. (1999) The essential DNA polymerases <5 and e are involved in repair of UV damaged DNA in the yeast Saccharomyces cerevisiae. Acta Biochim. Polon. 46, 289-298.
• Hamatake, R.K., Hasegawa, H., Clark, A.B., Bebenek, K., Kunkel, T.A. & Sugino, A. (1990) Purification and characterisation of DNA polymerase II from the yeast Saccharomyces cerevisiae. J. Biol Chem. 265, 4072-4083.
• Huang, J.C., Svoboda, D.L., Reardon, J.T. & Sancar, A. (1992) Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3 to the photodimer. Proc. Natl Acad Sci U.S.A. 89, 3664-3668.
• Husain, I., Griffith, J. & Sancar, A. (1988) Thy­mine dimers bend DNA. Proc. Natl, Acad. Sci U.S.A. 85, 2558-2562.
• Leem, S.H., Ropp, P.A. & Sugino, A. (1994) The yeast Saccharomyces cerevisiae DNA polymer­ase IV-possible involvement in double strand break DNA repair. Nucleic Acids Res. 22, 3011-3017.
• Meselson, M.S. & Radding, C.H. (1975) A general model for genetic recombination. Proc. Natl Acad Sci U.S.A. 72, 358-361.
• Morrison, A., Hiroyouki, A., Clark, A.B., Hama­take, R.R. & Sugino, A. (1990) A third essen­tial DNA polymerase in Saccharomyces cere­visiae. Cell 62, 1143-1151.
• Navas, T.A., Zhou, Z. & Elledge, S.J. (1995) DNA polymerase £ links the replication machinery to the S phase checkpoint. Cell 80, 29-39.
• Nelson, J.R., Lawrence, C.W. & Hinkle, D.C. (1996) Thymine-thymine dimer bypass by yeast DNA polymerase zeta. Science 272, 1646-1649.
• Pearlman, D.A., Holbrook, S.R., Pirkle, D.H. & Kim, S.H. (1985) Molecular models for DNA damaged by photoreaction. Science 227, 1304-1308.
• Schimizu, K., Santocanale, C., Ropp, P.A., Longhese, M.P., Plevani, P., Lucchini, G. & Sugino, A. (1993) Purification and characteri­sation of a new DNA polymerase from budding yeast Saccharomyces cerevisiae J. Biol Chem. 268, 27148-27153.
• Seeberg, E., Eide, L. & Bjoras, M. (1995) The base excision repair pathway. Trends Biochem. Scl 20, 391-397.
• Singer, B. & Kusmierek, J.T. (1982) Chemical mu­tagenesis. Annu. Rev. Biochem. 51, 655-693.
• Sitney, K.C., Budd, M.E. & Campbell, J.L. (1989) DNA polymerase III, a second essential DNA polymerase, is encoded by the Saccharomyces cerevisiae. Cell 56, 599-605.
• Sherman, F., Fink, G.R. & Lawrence, C.W. (1974; Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Sring Harbor, New York.
• Sobol, R.W., Horton, J.K., Kuhn, R., Gu, H., Singhal, R.K., Prasad, R., Rajewsky, K. & Wil­son, S.H. (1996) Requirement of mammalian DNA polymera8e-beta in base-excision repair. Nature 379, 183-186.
• Suszek, W., Baranowska, H., Żuk, J. & Jachymczyk, W J. (1993) DNA polymerase III is required for DNA repair in Saccharomyces cerevisiae. Curr. Genet. 24, 200-204.
• Szostak, J.W., Orr-Weaver, T.L., Rothstein, R.J. & Stahl, F.W. (1983) The double-strand-break re­pair model for recombination. Cell 33,25-35.
• Wang, Z., Wu, X. & Friedberg, E.C. (1993) DNA re­pair synthesis during base excision repair in vitro is catalysed by DNA polymerase e and is influenced by DNA polymerase a and ó in Saccharomyces cerevisiae. Mol Cell Biol 13, 1051-1058.
• Wiebauer, K. & Jiricny, J. (1990) Mis­match-specific thymine DNA glycosylase and DNA polymerase mediate the correction of G*T mispairs in nuclear extracts from human cells. Proc. Natl Acad. Sci. U.S.A. 87, 5842-5845.