Immunospecific nuclear proteins of colorectal tumors

• Autorzy: Szymczyk P. , Krajewska W.M. , Jakubik J. , Berner A. , Berner J. , Brocki M. , Rajczyk B. , Kilianska Z.
• Języki publikacji: EN

Abstrakty

EN

Electrophoretically specific nuclear proteins of human colon adenocarcinoma with mol. wt of 35-40 kDa were used as immunogen to produce rabbit antiserum. Expression of cancer-specific antigens was investigated by Western blot technique among nuclear proteins from normal and cancerous mucosa. Obtained antiserum crossreacted mainly with 36 kDa polypeptide in 23 of 26 (88,5%) colorectal tumor nuclear fractions but not with any of normal ones. It was also observed that this antiserum recognized 36 kDa antigen in 10 of 12 and 6 of 7 nuclear fractions from other cancers, ie. gastric and lung, respectively. In part of studied tumors antiserum crossreacted also with the antigens of 38 and 32-33 kDa. Expression of 36 and 32-33 kDa components seems to be correlated with colorectal cancer progression from A to B stage of disease according to the classification of Dukes. Immunoblot analysis revealed that cancer-specific 36 kDa polypeptide, mainly associated with nuclear compartment, can be also detected within 10P and 100P fractions of colorectal tumors.

Słowa kluczowe

EN

cellular fraction; antigen; rabbit antiserum; tumour marker; colorectal tumour; human cancer

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1996
• Tom: 01
• Numer: 4
• Opis fizyczny: p.363-371,fig.

Twórcy

autor

Szymczyk P.

• University of Lodz, Banacha 12-16, 90-237 Lodz, Poland

autor

Krajewska W.M.

autor

Jakubik J.

autor

Berner A.

autor

Berner J.

autor

Brocki M.

autor

Rajczyk B.

autor

Kilianska Z.

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