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1996 | 01 | 4 |
Tytuł artykułu

Immunospecific nuclear proteins of colorectal tumors

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Electrophoretically specific nuclear proteins of human colon adenocarcinoma with mol. wt of 35-40 kDa were used as immunogen to produce rabbit antiserum. Expression of cancer-specific antigens was investigated by Western blot technique among nuclear proteins from normal and cancerous mucosa. Obtained antiserum crossreacted mainly with 36 kDa polypeptide in 23 of 26 (88,5%) colorectal tumor nuclear fractions but not with any of normal ones. It was also observed that this antiserum recognized 36 kDa antigen in 10 of 12 and 6 of 7 nuclear fractions from other cancers, ie. gastric and lung, respectively. In part of studied tumors antiserum crossreacted also with the antigens of 38 and 32-33 kDa. Expression of 36 and 32-33 kDa components seems to be correlated with colorectal cancer progression from A to B stage of disease according to the classification of Dukes. Immunoblot analysis revealed that cancer-specific 36 kDa polypeptide, mainly associated with nuclear compartment, can be also detected within 10P and 100P fractions of colorectal tumors.
Wydawca
-
Rocznik
Tom
01
Numer
4
Opis fizyczny
p.363-371,fig.
Twórcy
autor
  • University of Lodz, Banacha 12-16, 90-237 Lodz, Poland
autor
autor
autor
autor
autor
autor
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-1d420b5e-8100-468b-a3ef-878290010526
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