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2007 | 63 | 04 |

Tytuł artykułu

Distribution and morphology of ChAT- and VAChT-immunoreactive neurons in the tuberal hypothalamus of the pig

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) are currently accepted specific markers for cholinergic neurons. Current knowledge concerning the organization of the hypothalamic cholinergic system has been derived predominantly from laboratory animals. In the present study, applying immunocytochemistry the authors investigated the morphology and distribution of the ChAT- and VAChT-immunoreactive nerve cells and terminals in the porcine hypothalamic preoptic, supraoptic and tuberal nuclei. ChAT-immunoreactive perikarya were present in the tuberal arcuate nucleus. Numerous VAChT- -positive terminals were found in the external layer of the median eminence, while perikarya occupied the perifornical, dorso-caudal and dorsal hypothalamic nuclei. These results provide morphological indications that the cholinergic hypothalamic system may affect the secretory function of the median eminence in pigs.

Wydawca

-

Rocznik

Tom

63

Numer

04

Opis fizyczny

p.412-415,fig.,ref.

Twórcy

autor
  • University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland
autor
autor
autor
autor

Bibliografia

  • 1.Arvidsson U., Riedl M., Elde R., Meister B.: Vesicular acetylcholine transporter (VAChT) protein: a novel and unique marker for cholinergic neurons in the central and peripheral nervous system. J. Comp. Neurol. 1997, 378, 454-467.
  • 2.Całka J., Kaleczyc J., Majewski M.: Histochemical demonstration of acetylcholinesterase (AChE) activity in some regions of the hypothalamus in the pig. Zool. Jb. Anat. 1993, 123, 187-196. 3.Feldberg W., Vogt M.: Acetylcholine synthesis in different regions of the central nervous system. J. Physiol. Lond. 1948, 107, 372-381.
  • 4.Grandison L., Gelato M., Meiter J.: Inhibition of prolactin secretion by cholinergic drugs. Proc. Soc. Exp. Biol. 1974, 145, 1236-1239.
  • 5.Hebb C. O., Silver A.: Choline acetylase in the central nervous system of man and some other mammals. J. Physiol. Lond. 1956, 134, 718-728.
  • 6.Ichikawa T., Ajiki K., Matsuura J., Misawa H.: Localization of two cholinergic markers, choline acetyltransferase and vesicular acetylcholine transporter in the central nervous system of the rat: in situ hybridization histochemistry and immunohistochemistry. J. Chem. Neuroanat. 1997, 13, 23-39. 7. Jones M. T., Hilhouse E. W., Burden J.: Effect of various putative neurotransmitters on the secretion of corticotropin-releasing hormone from the rat hypothalamus in vitro a model of the neurotransmitters involved. J. Endocrinol. 1976, 69, 1-10.
  • 8.Karnovsky M. J., Roots L.: A ”direct-coloring” thiocholine method for cholinesterases. J. Histochem. Cytochem. 1964, 12, 219-221.
  • 9.Kimura H., McGeer P. L., Peng J.-H.: Choline acetyltransferase-containing neurons in the rat brain, [in:] Bjorklund T., Hokfelt T., Kuhar M. J. (Eds.): Handbook of Chemical Neuroanatomy. Elsevier, Amsterdam 1984, pp. 51-67.
  • 10.Kizer J. S., Palkovits M., Tappaz M., Kebabian J., Brownstein M. J.: Distribution of releasing factors, biogenic amines, and related enzymes in the bovine median eminence. Endocrinology 1976, 98, 685-695.
  • 11.Kobayashi R. M., Brownstein M., Saavedra J. M., Palkovits M.: Choline acetyltransferase content in discrete regions of the brain stem. J. Neurochem. 1975, 24, 637-641.
  • 12.Levey A. I., Wainer B. H., Mufson E. J., Mesulam M.-M.: Co-localization of acetylcholinesterase and choline acetyltransferase in the rat cerebrum. Neuroscience 1983, 9, 9-22. 13.Libertun C., McCann S. M.: Blockade of the release of gonadotropins and prolactin by subcutaneous or intraventricular injection of atropine in male and female rats. Endocrinology 1973, 92, 1714-1724.
  • 14.Richardson S. B., Prasad J. A., Hollander C. S.: Acetylcholine, melatonin, and potassium depolarization stimulate release of luteinizing hormone-releasing hormone from rat hypothalamus in vitro. Proc. Natl. Acad. Sci. USA 1982, 79, 2686-2689.
  • 15.Swindle M. M., Moody D. C., Philips L. D.: Swine as models in biomedical research. Iowa State Univ. Press, Ames 1992, pp. 1-312.
  • 16.Szteyn S., Galert D., Dynowski J., Hoczyk W.: The sereotaxic configuration of hypothalamus nerve centres in the pig. Anat. Anz. 1980, 147, 12-32.
  • 17.Tago H., McGeer P. L., Bruce G., Hersh L. B.: Distribution of choline acetyltransferase-containing neurons of the hypothalamus. Brain Res. 1987, 415, 49-62.
  • 18.Wainer B. H., Levey A. I., Mufson E. J., Mesulam M.-M.: Cholinergic systems in mammalian brain identified with antibodies against choline acetyltransferase. Neurochem. Int. 1984, 6, 163-182. 19.Watson R. E., Wiegand S., Hoffman G.: Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides 1986, 7, 155-159.
  • 20.Weihe E., Tao-Cheng J.-H., Schafer M.-K., Erickson J., Eiden L. E.: Visualization of the vesicular acetylcholine transporter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles. Proc. Natl. Acad. Sci. USA 1996, 93, 3547-3552.
  • 21.Welento J.: Budowa i topografia jąder międzymózgowia świni. Cześć I, II, III, IV. Annal. Univers. Marie Curie-Sklodowska, Sectio DD, Lublin 1964, 19

Typ dokumentu

Bibliografia

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