EN
Choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) are currently accepted specific markers for cholinergic neurons. Current knowledge concerning the organization of the hypothalamic cholinergic system has been derived predominantly from laboratory animals. In the present study, applying immunocytochemistry the authors investigated the morphology and distribution of the ChAT- and VAChT-immunoreactive nerve cells and terminals in the porcine hypothalamic preoptic, supraoptic and tuberal nuclei. ChAT-immunoreactive perikarya were present in the tuberal arcuate nucleus. Numerous VAChT- -positive terminals were found in the external layer of the median eminence, while perikarya occupied the perifornical, dorso-caudal and dorsal hypothalamic nuclei. These results provide morphological indications that the cholinergic hypothalamic system may affect the secretory function of the median eminence in pigs.