Long term monitoring of Babesia microti infection in BALB-c mice using nested PCR

• Autorzy: Welc-Faleciak R. , Bajer A. , Bednarska M. , Paziewska A. , Sinski E.
• Języki publikacji: EN

Abstrakty

EN

In this study we report the usefulness of nested PCR for screening of the persistent B. microti infections in rodent hosts. Female BALB/c mice were inoculated with 100 μl of donor blood infected with B. microti. Infections were detected using microscopic examination of Giemsa-stained blood smears. To determine whether B. microti DNA was present in blood and/or spleen tissue, nested PCR was performed targeting a specifi c fragment of the gene encoding the 18S rRNA. Blood was sampled every 10 days post-infection (dpi) until day 30, after which mice were sampled every 30 days until the end of experiment at 360 dpi. The most extensive parasitaemia (39% of infected erythrocytes) was observed at 10 dpi. Between 20-60 dpi, less then 1% of infected erythrocytes were detected in blood smears, and from 90 dpi onwards, infected erythrocytes were no longer observed. B. microti DNA was successfully amplifi ed from the blood of mice from 10 dpi until 180 dpi, as well as from spleens of infected mice at 10 and 20 dpi. The presented results show that nested PCR is the method of choice for monitoring infections of B. microti in the blood of rodent hosts, and could therefore be a tool for environmental monitoring of naturally infected rodents which are the predominant source of infection for tick vectors.

Słowa kluczowe

EN

parasite; mouse; infection; mice; Babesia microti; blood; monitoring; BALB-c mice; Piroplasmida; rodent host; Apicomplexa

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2007
• Tom: 14
• Numer: 2
• Opis fizyczny: p.287-290,fig.,ref.

Twórcy

autor

Welc-Faleciak R.

• University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland

autor

Bajer A.

autor

Bednarska M.

autor

Paziewska A.

autor

Sinski E.

Bibliografia

• Bajer A, Pawelczyk A, Behnke JM, Gilbert FS, Sinski E: Factors affecting the component community structure of haemoparasites in bank voles (Clethrionomys glareolus) from the Mazury Lake District region of Poland. Parasitology 2001, 122, 43-54.
• Böse R, Jorgensen WK, Dalgliesh RJ, Friedhoff KT, de Vos AJ: Current state and future trends in the diagnosis of babesiosis. Vet Parasitol 1995, 57, 61-74.
• Burkot TR, Schneider BS, Pieniazek NJ, Happ CM, Rutherford JS, Slemenda SB, Hoffmeister E, Maupin GO, Zeidner NS: Babesia microti and Borrelia bissettii transmission by Ixodes spinipalpis ticks among prairie voles, Microtus ochrogaster, in Colorado. Parasitology 2000, 121, 595-599.
• Gray J, von Stedingk LV, Gurtelschmid M, Granstrom M: Transmission studies of Babesia microti in Ixodes ricinus ticks and gerbils. J Clin Microbiol 2002, 40, 1259-1263.
• Healing TD: Infections with blood parasites in the small British rodents Apodemus sylvaticus, Clethrionomys glareolus and Microtus agrestis. Parasitology 1981, 83, 179-189
• Hildebrandt A, Hunfeld KP, Baier M, Krumbholz A, Sachse S, Lorenzen T, Kiehntopf M, Fricke HJ, Straube E: First confi rmed autochthonous case of human Babesia microti infection in Europe. Eur J Clin Microbiol Infect Dis 2007, 8, 595-601.
• Homer MJ, Aguilar-Delfi n I, Telford III SR, Krause PJ, Persing DH: Babesiosis. Clin Microbiol Rev 2000, 13, 451-469.
• Konopka E, Sinski E: Experimental infection of mice with Babesia microti: characterization of parasitemia. Wiad Parazytol 1996, 42, 395-406.
• Mehlhorn H, Schein E: Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998. Parasitol Res 1998, 84, 467-475.
• Mitrović S, Kranjcić-Zec I, Arsić-Arsenijević V, Dzamić A, Radonjić I: Human babesiosis – recent discoveries. Med Pregl 2004, 57, 349-353.
• Pastusiak K, Konopka E, Doligalska M, Sinski E: Babesia microti: parasitaemia and antibody responses to primary and challenge infections in BALB/c mice. Folia Parasitol 2003, 50, 237-239.
• Pawelczyk A, Bajer A, Behnke JM, Gilbert FS, Sinski E: Factors affecting the component community structure of haemoparasites in common voles (Microtus arvalis) from the Mazury Lake District region of Poland. Parasitol Res 2004, 92, 270-284.
• Presing DH, Mathiensen D, Marshal WF, Telford SR, Spielman A, Thomford JW, Conrad PA: Detection of Babesia microti by ploymerase chain reaction. J Clin Microbiol 1992, 30, 2097-2103.
• Popovsky MA: Transfusion-transmitted babesiosis. Transfusion 1991, 31, 296-298.
• Pucek Z, Ryszkowski L, Zejda J: Estimation of average length of life in bank vole Clethrionomys glareolus (Schreber, 1780). In: Petrusewicz K, Ryszkowski P (Eds): Energy fl ow through small mammals populations. Warsaw 1970, PWN, 187-201.
• Ruebush MJ, Hanson WL: Susceptibility of fi ve strains of mice to Babesia microti of human origin. J Parasitol 1979, 65, 430-433.
• Sebek Z, Rosicky B, Sixl W: The occurrence of babesiasis affecting small terrestrial mammals and the importance of this zoonosis in Europe. Folia Parasitol 1977, 24, 221-228.
• Sinski E, Bajer A, Welc R, Pawelczyk A, Ogrzewalska M, Bhenke JM: Babesia microti: prevalence in wild rodents and Ixodes ricinus ticks from the Mazury Lakes District of North-Eastern Poland. Int J Med Microbiol 2006, 296, 137-143.
• Spielman A, Etkind P, Piesman J, Ruebush TK 2nd, Juranek DD, Jacobs MS: Reservoir hosts of human babesiosis on Nantucket Island. Am J Trop Med Hyg 1981, 30, 560-565.
• Stowell PCh, Gelfand JA, Shepard JAO, Kratz A: Case 17-2007: a 25-year-old woman with relapsing fevers and recent onset of dyspnea. N Eng J Med 2007, 356, 2313-2319.
• Tsuji M, Wei Q, Zamoto A, Morita C, Arai S, Shiota T, Fujimagari M, Itagaki A, Fujita H, Ishihara C: Human babesiosis in Japan: epizootiologic survey of rodent reservoir and isolation of new type of Babesia microti-like parasite. J Clin Microbiol 2001, 39, 4316-4322.
• Watkins RA, Moshier SE, O’Dell WD, Pinter AJ: Splenomegaly and reticulocytosis caused by Babesia microti infections in natural populations of the montane vole, Microtus montanus. J Protozool 1991, 38, 573-576.
• Wei Q, Tsuji M, Zamoto A, Kohsaki M, Matsui T, Shiota T, Telford SR 3rd, Ishihara C: Human babesiosis in Japan: isolation of Babesia microti-like parasites from an asymptomatic transfusion donor and from a rodent from an area where babesiosis is endemic. J Clin Microbiol 2001, 39, 2178-2183.
• Wójcik-Falta A, Cisak E, Chmielewska-Badora J, Zwoliński J, Buczek A, Dutkiewicz J: Prevalence of Babesia microti in Ixodes ricinus ticks from Lublin region (Eastern Poland). Ann Agric Environ Med 2006, 13, 319-322.
• Zamoto A, Tsuji M, Kawabuchi T, Wei Q, Asakawa M, Ishihara C: U.S.-type Babesia microti isolated from small wild mammals in Eastern Hokkaido, Japan. J Vet Med Sci 2004, 66, 919-926.