Effects of phytoestrogen daidzein and estradiol on steroidogenesis and expression of estrogen receptors in porcine luteinized granulosa cells from large follicles

• Autorzy: Nynca A. , Jablonska O. , Slomczynska M. , Petroff B.K. , Ciereszko R.E.
• Języki publikacji: EN

Abstrakty

EN

The aims of the study were to compare the in vitro effects of daidzein or 17ß-estradiol (E2) on: 1) progesterone (P4) secretion by luteinized granulosa cells harvested from large porcine follicles, as well as 2) estrogen receptor and ß (ER and ERß) mRNA and protein expression in the cells. In addition, the effect of daidzein on E2 secretion and viability of the granulosa cells was examined. We found that basal and gonadotropin-stimulated P4 secretion were inhibited in granulosa cells cultured in the presence of daidzein either for 24 or 48 hours. In contrast to daidzein, E2 reduced P4 secretion only during 24-hour cell cultures increasing it during longer cultures. Daidzein did not affect E2 secretion by granulosa cells. The expression of ER and ERß mRNA, as well as ERß protein, was up-regulated by daidzein but unaffected by E2. To conclude, the soy estrogen daidzein acts directly on the porcine ovary to decrease progesterone production and to increase expression of ERß mRNA and protein. Daidzein actions in porcine luteinized granulosa cells differ from those of estradiol and it may suggest disadvantageous effects of the phytoestrogen on reproductive processes in females.

Słowa kluczowe

EN

estradiol; mRNA; porcine follicle; protein expression; estrogen receptor expression; estrogen receptor beta; luteinized granulosa cell; progesterone; granulosa cell; daidzein; steroidogenesis; 17beta-estradiol; phytoestrogen; estrogen receptor alpha

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 2
• Opis fizyczny: p.95-105,fig.,ref.

Twórcy

autor

Nynca A.

• University Warmia and Mazury, Oczapowskiego 1A, 10-719 Olsztyn, Poland

autor

Jablonska O.

autor

Slomczynska M.

autor

Petroff B.K.

autor

Ciereszko R.E.