The influence of black currant [Ribes nigrum] seed extract on effectiveness of human ceruloplasmin in Fe[II] ions elimination

• Autorzy: Budzyn M , Iskra M. , Wielkoszynski T. , Malecka M. , Gryszczynska B.

Warianty tytułu

PL

Wplyw ekstraktu z pestek porzeczki czarnej [Ribus nigrum] na skutecznosc ludzkiej ceruloplazminy w eliminowaniu Fe[II]

• Języki publikacji: EN

Abstrakty

EN

Ions of irons, especially ferrous ions may be harmful for living organisms, because they generate reactive oxygen species like O2 •- or •OH. Probability of the risk rises especially in pathological conditions, in which high level of iron is observed. For this reason scientists try to establish new methods that can support organism in eliminating reactive ferrous ions. Nowadays, attention focuses on substances present in plants, especially polyphenols, whose administration prevents oxidative damages in iron overloading. This new approach requires some research on behavior of plant-derived compounds in human organism, within a system containing other biomolecules involved in iron metabolism. The aim of this study has been to investigate the influence of black currant (Ribes nigrum) seed extract, a source of polyphenols, on the activity of ceruloplasmin, an enzyme participating in Fe(II) elimination from blood plasma in human organism. Depletion of Fe(II) caused by ceruloplasmin isolated from healthy blood donors was compared to its decrease in a system containing both ceruloplasmin and the extract. The results have shown that addition of a particular amount of the extract elevates the effectiveness of ceruloplasmin in eliminating Fe(II) from the sample but only under physiological condition (pH 7.4; T 37°C). In a weak acidic solution, addition of the extract does not lead to any change in Fe(II) concentration.

PL

Jony żelaza, szczególnie jony żelazawe Fe(II), ze względu na wysoki potencjał oksydoredukcyjny mają zdolność generowania wolnych rodników, takich jak O2 •- i •OH. W pewnych stanach patologicznych, którym towarzyszy przeładowanie organizmu żelazem, ryzyko pojawienia się wolnych jonów Fe(II) inicjujących wiele procesów wolnorodnikowych jest bardzo prawdopodobne. W tym celu dąży się do opracowania terapii, które w chorobach żelazozależnych wspomogą naturalne mechanizmy usuwania reaktywnych jonów żelaza z organizmu. Ostatnio dużą uwagę poświęca się aktywnym substancjom obecnym w roślinach, szczególnie związkom polifenolowym, ze względu na ich silne właściwości chelatujące. Ten nowy kierunek badań wymaga jednak wnikliwej analizy, która wyjaśni mechanizm współdziałania substancji roślinnych z innymi biomolekułami zaangażowanymi w prawidłowy metabolizm żelaza w organizmie oraz rozstrzygnie o bezpieczeństwie ich stosowania. Celem badań było ustalenie wpływu ekstraktu z pestek porzeczki czarnej (Ribus nigrum), bogatego w związki polifenolowe, na aktywność ludzkiej ceruloplazminy, enzymu uczestniczącego w usuwaniu Fe(II) w organizmie ludzkim. Ubytek Fe(II) obserwowany w obecności ceruloplazminy porównywano z jego ubytkiem w mieszaninie ceruloplazmina-ekstrakt. Stwierdzono, że dodatek ekstraktu podnosi skuteczność ceruloplazminy w eliminowaniu Fe(II) ze środowiska. Istnieje więc przypuszczenie, że związki pochodzenia roślinnego takie jak polifenole mogą wspomagać naturalne mechanizmy eliminowania Fe(II) w organizmie.

Słowa kluczowe

PL

Ribes nigrum; black currant; seed; seed extract; ceruloplasmin; human ceruloplasmin; iron ion; elimination; effectiveness

• Wydawca: -
• Czasopismo: Journal of Elementology
• Rocznik: 2009
• Tom: 14
• Numer: 2
• Opis fizyczny: p.217-227,fig.,ref.

Twórcy

autor

Budzyn M

• Poznan University of Medical Science, 6 Grunwaldzka Street, 60-789 Poznan, Poland

autor

Iskra M.

autor

Wielkoszynski T.

autor

Malecka M.

autor

Gryszczynska B.

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