Typy fagowe oraz profile plazmidowego i chromosomalnego DNA szczepow Salmonella enterica subsp. enterica ser. Enteritidis [S.enteritidis] izolowanych w Polsce w latach 1999-2000

• Autorzy: Cieslik A. , Brown D. , Paciorek J. , Szych J. , Kaluzewski S.
• Języki publikacji: PL

Abstrakty

PL

Scharakteryzowano 140 szczepów S. Enteritidis określając ich lekowrażliwość, typując bakteriofagami wg Ward, oraz wyznaczając elektroforetyczne profile plazmidowego i chromosomalnego DNA. Izolowane w Polsce w latach 1999-2000 szczepy S. Enteritidis będące przedmiotem niniejszego opracowania w przeważającej większości przedstawiają typ fagowy 4, elektroforetyczne profile chromosomalnego DNA SentX1 i SentS1 oraz posiadają jeden plazmid o wielkości 57 kb.

EN

Salmonella Enteritidis strains are the most often isolated Salmonella serovars in Poland. In the present study, phage typing, plasmid profile analysis, and PFGE have been applied to characterize 140 Polish S. Enteritidis isolates originated from human cases of salmonellosis and from other sources. The typing phages of Ward and colleagues scheme were used to type a total of 140 S. Enteritidis strains coming from Poland. All 140 strains were typable and six different phage types were observed. A total of 125 (89%) of 140 isolates examined belonged to PT 4. The others PTs were represented by small amount of strains (PT1-2, PT6-6, PT7-1, PT8-4 and PT21-2 strains). Among all tested isolates six different plasmid profiles were observed. Of the 140 examined strains, 128 (91.4%) contained the 57 kb plasmid alone. After XbaI digestion four distinct pulse field chromosomal restriction profiles among studied S. Enteritidis were observed. XbaI and SpeI chromosomal restriction profiles of S. Enteritidis PT4 were identical with reference strain profiles. Our findings confirmed earlier suggestions that the increase of human salmonellosis cases in Poland was caused by S. Enteritidis PT4 and was due to consumption of contaminated food. This study confirmed the importance of using PFGE in combination with phage typing, plasmid typing and antibiotic resistance testing for studying the epidemiology of S. Enteritidis.

Słowa kluczowe

PL

bakteriofagi; wrazliwosc na leki; zakazenia przewodu pokarmowego; choroby czlowieka; szczepy bakteryjne; DNA chromosomowy; Salmonella enteritidis; Salmonella enterica subsp.enterica; DNA plazmidowe

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 2
• Opis fizyczny: s.185-196,fot.,bibliogr.

Twórcy

autor

Cieslik A.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Brown D.

autor

Paciorek J.

autor

Szych J.

autor

Kaluzewski S.

Bibliografia

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