DNA damage in human colonic mucosa cells induced by bleomycin and the protective action of vitamin E

• Autorzy: Wozniak K , Arabski M. , Malecka-Panas E. , Drzewoski J. , Blasiak J.
• Języki publikacji: EN

Abstrakty

EN

Using the alkaline comet assay, we showed that bleomycin at 0.1-5 μg/ml induced DNA strand breaks and/or alkali-labile sites, measurable as the comet tail moment, in human colonic mucosa cells. This DNA damage was completely repaired during a 120-minute post-treatment incubation of the cells. Post-treatment of the bleomycin-damaged DNA with 3-methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing alkylated bases, gave rise to a significant increase in the extent of DNA damage, indicating that the drug could induce alkylative bases in DNA. We did not observe any change in the comet tail moment in the presence of catalase. Vitamin E ((+)-α-tocopherol) decreased DNA damage induced by bleomycin. The results obtained suggest that hydrogen peroxide might not be involved in the formation of DNA lesions induced by bleomycin in the colonic mucosa cells, and that vitamin E may exert protective effects on these cells.

Słowa kluczowe

EN

vitamin E; mucosa cell; man; catalase; DNA damage; colonic mucosa; bleomycin; protective activity

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 1
• Opis fizyczny: p.31-45,fig.,ref.

Twórcy

autor

Wozniak K

• University of Lodz, Banacha 12-16, 90-237 Lodz, Poland

autor

Arabski M.

autor

Malecka-Panas E.

autor

Drzewoski J.

autor

Blasiak J.

Bibliografia

• 1. Croke, S.T. and Bradner, W.T. Bleomycin: A review. J. Med. 7 (1976) 333-428.
• 2. Xu, R.X., Nettesheim, D., Otvos, J.D. and Petering, D.H. NMR determination of the structures of peroxycobalt(III) bleomycin and cobalt(III) bleomycin, products of the aerobic oxidation of cobalt(II) bleomycin by dioxygen. Biochemistry 33 (1994) 907-916.
• 3. Caceres-Cortes, J., Sugiyama, H., Ikudome, K., Saito, I. and Wang, A.H.J. Structures of cobalt(III)-pepleomycin and cobalt(III)-deglyco-pepleomycin (green forms) determined by NMR studies. Eur. J. Biochem. 244 (1997) 818-828.
• 4. Burger, R.M. Nature of activated bleomycin. Struc. Bonding 97 (2000) 287-303.
• 5. Giloni, L., Takeshita, M., Johnson, F., Iden, C. and Grollman, A.P. Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage. J. Biol. Chem. 256 (1981) 8608-8615.
• 6. Wu, J. C., Stubbe, J. A. and Kozarich, J. W. Mechanism of bleomycin: evidence for 4'-ketone formation in poly(dA-dU) associated exclusively with free base release. Biochemistry 24 (1985) 7569-7573.
• 7. Sugiyama, H., Xu, C., Murugesan, N., Hecht, S.M., van der Mrel, G.A. and van Boom, J.H. Chemistry of the alkali-labile lesion formed from iron(II) bleomycin and d(CGCTTTAAAGCG). Biochemistry 27 (1988) 58-67.
• 8. Burger, R.M., Peisach, J. and Horwitz, S.B. Effects of O2 on the reactions of activated bleomycin. J. Biol. Chem. 257 (1982) 3372-3375.
• 9. Dziegielewski, J., Melendy, T. and Beerman, T.A. Bleomycin-induced
• alternations in DNA replication: relationship to DNA damage. Biochemistry 40 (2001) 704-711.
• 10. McKelvey-Martin, V.J., Green, M.H.L., Schmezer, P., Pool-Zobel, B.L., De Meo, M.P. and Collins, A. The single cell gel electrophoresis assay (Comet assay): A Europian review. Mutat. Res. 288 (1993) 47-63.
• 11. Pool-Zobel, B.L. and Leucht, U. Induction of DNA damage by risk factor of colon cancer in human colon cells derived from biopsies. Mutat. Res. 375 (1997) 105-115.
• 12. Singh, N.P., McCoy, T., Tice, R.R. and Schneider, E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-192.
• 13. Collins, A.R., Duthie, S. J. and Dobson, V.L. Direct enzymatic detection of endogenous base damage in human lymphocyte DNA. Carcinogenesis 14 (1993) 1733-1735.
• 14. Klaude, M., Eriksson, S., Nygren, J. and Ahnstrom, G. The comet assay: Mechanisms and technical considerations. Mutat. Res. 363 (1996) 89-96.
• 15. Blasiak, J. and Kowalik, J. A comparison of the in vitro genotoxicity of tri- and hexavalent chromium. Mutat. Res. 469 (2000) 135-145.
• 16. Tudek, B., Van Zeeland, A.A., Kusmierek, J.T. and Laval, J. Activity of Escherichia coli DNA-glycosylases on DNA damaged by methylating and ethylating agents and influence of 3-substituted adenine derivatives. Mutat. Res. 407 (1998) 169-176.
• 17. Jahn, B. and Hansch, G.M. Oxygen radical generation in human platelets: dependence of 12-lipoxygenase activity and on the glutathione cycle. Int. Arch. Allergy Appl. Immunol. 93 (1990) 73-79.
• 18. Chu, F.F. and Esworthy, R.S. The expression of an intestinal form of glutathione peroxidase in rat intestinal epithelium. Arch. Biochem. Biophys. 323 (1995) 288-294.
• 19. Frederiks, W.M. and Bosch, K.S. Localization of superoxide dismutase in rat tissues. Free Radical Biol. Med. 22 (1997) 241-248.
• 20. Tham, D.M., Whitin, J.C., Kim, K.K., Zhu, S.X. and Cohen, H.J. Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract. Am. J. Physiol. 275 (1998) G1463-G1471.
• 21. Blau, S., Rubinstein, A., Bass, P., Singaram, C. and Kohen, R. Differences in the reducing power along the rat GI tract: lower antioxidant capacity of the colon. Mol. Cell. Biochem. 194 (1999) 185-191.
• 22. Halliwell, B., Zhao, K. and Whiteman, M. The gastrointestinal tract: A major site of antioxidant action? Free Radical Res. 33 (2000) 819-830.
• 23. Levine, M., Rumsey, S.C., Daruwala, S., Park, J.B. and Wang, Y. Criteria and recommendations for vitamin C intake. J. Am. Med. Assoc. 281 (1999) 1415-1423.
• 24. Traber, M.G. and Arai, H. Molecular mechanisms of vitamin E transport. Ann. Rev. Nut. 19 (1999) 343-355.
• 25. Roxborough, H.E., Burton, G.W. and Kelly, F.J. Inter and intra-individual variation in plasma and red blood cell vitamin E after supplementation. Free Radical Res. 33 (2000) 437-445.
• 26. Tada, T., Matsumoto, K. and Suzuki, H. Electrochemotherapy siginficantly inhibits the growth of colon 26 tumors in mice. Surg. Today 27 (1997) 506-510.
• 27. Kato, M., Ioritani, N., Suzuki, T., Kambe, M., Inaba, Y., Watanabe, R., Sasano, H. and Orikasa, S. Mechanism of anti-tumor effect of combination of bleomycin and shock waves. Jpn. J. Cancer Res. 91 (2000) 1065-1072.
• 28. Kuriyama, S., Tsujinoue, H., Toyokawa, Y., Mitoro, A., Nakatani, T., Yoshiji, H., Tsujimoto, T. and Fukui, H. A potential approach for electrochemotherapy against colorectal carcinoma using a clinically available alternating current system with bipolar snare in a mouse model. Scand. J. Gastroenterol. 36 (2001) 297-302.
• 29. Byrnes, R.W., Templin, J., Sem, D., Lyman, S. and Petering, D.H. Intracellular DNA strand scission and growth inhibition of Ehrlich ascites tumor cells by bleomycins. Cancer Res. 50 (1990) 5275-5286.
• 30. Byrnes, R.W. and Petering, D.H. DNA strand breakage in isolated nuclei subjected to bleomycin or hydrogen peroxide. Biochem. Pharmacol. 48 (1994) 575-582.
• 31. Ejchart, A. Genotoxity of bleomycin in human cell lines differing in catalase activity. Acta Pol. Pharm. 1 (2001) 25-29.
• 32. Chakrabarti, S., Makrigiorgos, G.M., O’Brien, K., Bump, E. and Kassis, A.I. Measurement of hydroxyl radicals catalyzed in the immediate vicinity of DNA by metal-bleomycin complexes. Free Radic. Biol. Med. 20 (1996) 777-783.
• 33. Krokan, H.E., Standal, R. and Slupphaug, G. DNA glycosylases in the base excision repair. Biochem. J. 325 (1997) 1-16.
• 34. Kline, K.K., Weiping, Y. and Sanders, B.G. Vitamin E: mechanisms of action as tumor cell growth inhibitors. J. Nutr. 131 (2001) 161-163.
• 35. Yamashita, N., Murata, M., Inoue, S., Burkitt, M., Milne, L. and Kawanishi, S.
• α-Tocopherol induces oxidative damage to DNA in the presence of copper (II) ions. Chem. Res. Toxicol. 11 (1998) 855-862.
• 36. Suntres, Z.E. and Shek, P.N. Protective effect of liposomal alpha-tocopherol against bleomycin-induced lung injury. Biomed. Environ. Sci. 10 (1997) 47-59.
• 37. Kilinc, C., Ozcan, O., Karaoz, E., Sunguroglu, K., Kutluay, T. and Karaca, L. Vitamin E reduces bleomycin-induced lung fibrosis in mice biochemical and morphological studies. J. Basic Clin. Physiol. Pharmacol. 4 (1993) 249-269.
• 38. Mahabir, S., Coit, D., Liebes, L., Brady, M.S., Levis, J.J., Roush, G., Nestle, M., Fry, D. and Berwick, M. Randomized, placebo-controlled trial of dietary supplementation of alpha-tocopherol on mutagen sensitivity levels in melanoma patients: a pilot trial. Melanoma Res. 12 (2002) 83-90.