Optimized methods for stabilization of microbal communities specializing in biodegradation of organic environmental contaminants

• Autorzy: Augustynowicz J , Kaszycki P. , Kus M. , Bialecka A. , Koloczek H.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to optimize storage conditions of a microbial community used for degradation of petroleum-derived environmental contaminants. Microorganisms were either freeze-dried or directly frozen (-20°C) in the presence of four stabilizers: trehalose, sucrose, glycerol and DMSO. It was found that preincubation with trehalose and sucrose had a positive impact on cell viability for both tested storage techniques. Disaccharide-stabilized consortia were more biodiverse than control samples (untreated with any protectants) and they retained high xenobiotic biodegradation capabilities. The effect of glycerol and DMSO was unexpectedly poor, contradicting other findings on the protective action of these compounds on monocultures. Higher cell survival was achieved only upon short-term (7 days) freezing, whereas DMSO proved to be lethal in the case of freeze-dried communities. Taking into account practical and economic reasons, the use of sucrose rather than the more expensive trehalose appears as the most efficient method for microbial consortia biostabilization during long-term storage. The experimental work provides some important data concerning the problem of elaboration of improved methods for preserving robust microbial communities to be used in environmental biotechnology practice.

Słowa kluczowe

EN

microbial community; biodegradation; organic contaminant; environment contaminant; biodiversity; petroleum-derived xenobiotic; microorganism survival; freezing

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2008
• Tom: 17
• Numer: 5
• Opis fizyczny: p.655-664,fig.,ref.

Twórcy

autor

Augustynowicz J

• University of Agriculture, Al.29 Listopada 54, 31-425 Krakow, Poland

autor

Kaszycki P.

autor

Kus M.

autor

Bialecka A.

autor

Koloczek H.

Bibliografia

• 1. LEAHY J.G., COLWELL R.R. Microbial degradation of hydrocarbons in the environment. Microbiol. Rev. 54 (3), 305, 1990.
• 2. MULLER-MARGRAF W. Bioremediation of soil. Methods in Applied Soil Microbiology and Biochemistry; Academic Press Ltd., Chpt. 11, 529, 1995.
• 3. SURYGAŁA, J. Petroleum contaminants in ground. Oficyna Wyd. Politechniki Wrocławskiej, Wrocław 2000 [In Polish].
• 4. BOSZCZYK-MALESZAK H., CHORĄŻY M., BIESZKIEWICZ E., KACIESZCZENKO J. Phenol utilisation by fungi isolated from activated sludge. Acta Microbiol. Polon. 51, 183, 2002.
• 5. van HAMME J.D., SINGH A., WARD O.P. Recent advances in petroleum microbiology. Microbiol. Mol. Biol. Rev. 67 (4), 503, 2003.
• 6. OKOH A.I. Biodegradation of Bonny light crude oil in soil microcosm by some bacterial strains isolated from crude oil flow stations saver pits in Nigeria. Afr. J. Biotechnol. 2, 104, 2003.
• 7. TOWNSEND G.T., PRINCE R.C., SUFLITA J.M. Anaerobic oxidation of crude oil hydrocarbons by the resident microorganisms of a contaminated anoxic aquifer. Environ. Sci. Technol. 37, 5213, 2003.
• 8. VINAS M., GRIFOLL M., SABATE J., SOLANAS A.M. Biodegradation of a crude oil by three microbial consortia of different origins and metabolic capabilities J. Indust. Microbiol. Biotechnol. 28, 252, 2002.
• 9. KASZYCKI P., SZUMILAS P., KOŁOCZEK H. Microbial consortium used for bioremediation of environmental pollution with hydrocarbons and their derivatives. Inżynieria Ekologiczna 4, 15, 2001 [In Polish].
• 10. KOŁOCZEK H., KASZYCKI P. Bioremediation of refinery-pollutants in ground-water environment. In: Methods of hydrocarbon contaminants removal from ground-water environment. Ed. S. Rychlicki, AGH Uczelniane Wydawnictwa Naukowo-Dydaktyczne, Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie, pp.149, Kraków 2006 [In Polish].
• 11. PETRYSZAK P., KASZYCKI P., KOŁOCZEK H. Synergic effect of biphenyl biodegradation by microbial consortia. J. Indust. Microbiol. Biotechnol., 2008 (in preparation).
• 12. EUROPEAN NORM EN 12322:1999 (PN-EN 12322:2002) In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media.
• 13. MIYAMOTO-SHINOHARA Y., IMAIZUMI T., SUKENOBE J., MURAKAMI Y., KAWAMURA S., KOMATSU Y. Survival rate of microbes after freeze-drying and long-term storage. Cryobiology 41, 251, 2002.
• 14. SHARMA B., SMITH D. Recovery of fungi after storage for over a quarter of a century. World J. Microbiol. Biotechnol. 15, 517, 1999.
• 15. TANGHE A., van DIJCK P., THEVELEIN J.M. Determination of freeze tolerance in microorganisms, physiological importance, and biotechnological applications. Adv. Appl. Microbiol. 53, 129, 2003.
• 16. WOLFE J., BRYANT G. Cellular Cryobiology: thermodynamic and mechanical effects. International Journal of Refrigeration 24, 438, 2001.
• 17. CROWE L.M. Lessons from nature: the role of sugars in anhydrobiosis. Com. Biochem. Physiol. 131A, 505, 2002.
• 18. STOREY K.B. Organic solutes in freezing tolerance. Comp. Biochem. Physiol. 117A, 319, 1997.
• 19. HUBALEK Z. Protectants used in the cryopreservation of microorganisms. Cryobiology 46, 205, 2003.
• 20. ARGÜELLES J.C. Physiological roles of trehalose in bacteria and yeasts. A comparative analysis. Arch. Microbiol. 174 (4), 217, 2000.
• 21. CLEGG J.S. Cryptobiosis – a peculiar state of biological organization. Comp. Biochem. Physiol. 128B, 613, 2001.
• 22. CROWE J.H., CARPENTER J.F., CROWE L.M. The role of vitrification in anhydrobiosis. Annu. Rev. Physiol. 60, 73, 1998.
• 23. AUGUSTYNOWICZ J., SZARANIEC B., KASZYCKI P., KOŁOCZEK H. Influence of trehalose on the stability of microbial communities used for degradation of petroleum derivatives. Acta Sci. Polon. Biotechnol. 3 (1-2), 3, 2004 [In Polish].
• 24. KASZYCKI P., PETRYSZAK P., KOŁOCZEK H. Microbial bioremediation of oily waste: construction of communities based on autochthonous microorganisms. World J. Microbiol. Biotechnol. 2008 (in print).
• 25. SCHIRALDI C., DI LERNIA I., DE ROSA M. Trehalose production: exploiting novel approaches. Trends Biotechnol. 20, 420, 2002.
• 26. WOLKERS W.F., WALKER N.J., TABLIN F., CROWE J.H. Human platelets loaded with trehalose survive freezedrying. Cryobiology 42, 79, 2001.
• 27. SMITH D. Provision and maintenance of microorganisms for industry and international research networks. CryoLetters 22, 91, 2001.
• 28. SMITH D., RYAN M.J. Current status of fungal collections and their role in biotechnology. In Handbook of Fungal Biotechnology. Ed. Marcel Dekker, Inc., pp 527-538, 2003.
• 29. EUROPEAN NORM EN 12353:2007 (PN-EN 12353:2002) Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, mycobactericidal, sporicidal and fungicidal activity.
• 30. REICHGOTT M. Reference strains: How many passages are too many? ATCC Connection 23 (2), Winter 2003.
• 31. LESLIE S., ISRAELI E., LIGHTHART B., CROWE J.H., CROWE L.M. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying. Applied and Environmental Microbiology 61 (10), 3592, 1995.
• 32. http://www.lgcpromochem-atcc.com
• 33. WOLFE J., BRYANT G. Freezing, drying and/or vitrification of membrane-solute-water systems. Crybiology 39, 103, 1999.
• 34. RALL W.M., FAHY G.M. Ice free cryopreservation of mouse embryos at -196°C by vitrification. Nature 313, 573, 1985.
• 35. TROJANOWICZ K., DUSZA P., KASZYCKI P., KUBICA M., KOŁOCZEK H. Application of a microbial community for construction of a specialized activated sludge. Characteristics of biodegradation process in a model wastewater-treatment system of “Glimar” S.A. oil refinery. Inżynieria Ekologiczna 4, 52, 2001 [In Polish].
• 36. AMANN R.I., LUDWIG W., SCHLEIFER K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143, 1995.
• 37. LOVLEY D.R. Cleaning up with genomics: Applying molecular biology to bioremediation. Nature Rev. Microbiol. 1, 35, 2003.
• 38. MAŁACHOWSKA-JUTSZ A., MROZOWSKA J. Enzymatic activity in soil contaminated with oil-derived compounds during detoxication process. Biotechnologia 1 (36), 79, 1997 [In Polish].