Naltrexone stimulates LH secretion in vitro from the common carp [Cyprinus carpio L.] pituitary gland

• Autorzy: Socha M , Sokolowska-Mikolajczyk M , Mikolajczyk T , Chyb J , Epler P
• Języki publikacji: EN

Abstrakty

EN

The effects of naltrexone on the in vitro luteinizing hormone (LH) secretion from whole pituitary glands as well as from dispersed pituitary cells of common carp (Cyprinus carpio L.)in primary cell culture were studied. The perifusion of whole pituitary glands with naltrexone at a concentration of 10⁻⁴ M caused a significant increase of LH secretion compared to the control. This finding suggests that opioid peptides can indirectly affect LH secretion at the pituitary level, probably through the nerve terminals containing GnRH and/or DA present in the pituitary. In the perifusion of dispersed pituitary cells with naltrexone at concentrations of 10⁻⁶ or 10⁻⁴ M, significant increases of LH levels were found. Similar results were observed in static culture of dispersed carp pituitary cells: after 4 or 24 hours of culture in the medium containing naltrexone at concentrations of 10⁻⁶, 10⁻⁴ or 10⁻² M,a dose-dependent, statistically significant increase of LH secretion to the culture medium was found. The results from dispersed pituitary cell perifusions or cell culture with naltrexone indicate that this antagonist directly affects the pituitary cells and stimulates LH release from carp gonadotrophs.

PL

Celem niniejszej pracy było zbadanie wpływu naltreksonu na wydzielanie LH z całych przysadek mózgowych, jak również ze zdyspersowanych komórek przysadki mózgowej karpia. Peryfuzja całych przysadek mózgowych medium o koncentracji naltreksonu 10⁻⁴ M spowodowała istotny wzrost poziomu LH w medium w porównaniu z kontrolą (rys. 2). Wyniki te sugerować mogą pośrednie działanie peptydów opioidowych na sekrecje LH na poziomie przysadki mózgowej, prawdopodobnie poprzez obecne w przysadce zakończenia nerwowe, zawierające GnRH i/lub dopaminę. Istotny wzrost sekrecji LH obserwowano także ze zdyspersowanych komórek przysadki mózgowej pod wpływem naltreksonu (rys. 3). Zarówno w trakcie peryfuzji (Nalt 10⁻⁶,10⁻⁴ M), jak i statycznych hodowli (rys. 4) in vitro (Nalt 10⁻⁶,10⁻⁴ lub 10⁻² M). Wyniki doświadczeń in vitro (peryfuzje, hodowle komórkowe) wykonanych na zdyspersowanych komórkach przysadki mózgowej wskazują, że naltrekson bezpośrednio oddziałuje na komórki przysadki mózgowej i stymuluje uwalnianie LH z gonadotropów.

Słowa kluczowe

EN

pituitary cell; carp; fish; pituitary gland; Cyprinus carpio; culture medium; luteinizing hormone; naltrexone; common carp; in vitro; cell culture; secretion

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2003
• Tom: 11
• Numer: 1
• Opis fizyczny: p.5-16,fig.,ref.

Twórcy

autor

Socha M

• Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Krakow-Mydlinki, Poland

autor

Sokolowska-Mikolajczyk M

• Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Krakow-Mydlinki, Poland

autor

Mikolajczyk T

• Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Krakow-Mydlinki, Poland

autor

Chyb J

• Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Krakow-Mydlinki, Poland

autor

Epler P

• Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Krakow-Mydlinki, Poland

Bibliografia

• Ball J.N. 1981 - Hypothalamic control of the pars distalis in fishes, amphibians, and reptiles - Gen. Comp. Endocrinol. 44: 135-170.
• Barb C.R., Barret J.B., Wright J.T., Kraeling R.R.,and Rampacek G.B. 1990 - Opioid modulation of LH secretion by pig pituitary cells in vitro - J. Reprod. Fertil. 1: 213-219.
• Bird D.J., Buckingham J.C., Baker B., Mukherjee S. 1987 - Hypothalamo-pituitary-interrenal responses to opioid substances in the trout. I. Effects of morphine on the release in vitro of corticotrophin-releasing activity from the hypothalamus and corticotrophin from the pituitary gland - Gen. Comp. Endocrinol. 68: 33-39.
• Bird D.J., Jackson M., Baker B.J., Buckingham J.C. 1988 - Opioid binding sites in the fish brain: an autoradiographic study - Gen. Comp. Endocrinol. 70: 49-62.
• Blank M.S., Fabbri A., Catt K.J., Dufau M.L. 1986 - Inhibition of luteinizing hormone release by morphine and endogenous opiates in cultured pituitary cells - Life Sci. 38: 2097-2101.
• Bonavera J.J., Sahu A., Kalra S.P., Kalra P.S. 1994 - The hypothalamic peptides, β-endorphin, neuropeptide K and interleukin-1β, and the opiate morphine, enhance the excitatory amino acid-induced LH release under the influence of gonadal steroids - Neuroendocrinology 6: 557-564.
• Cacicedo L., Sanchez-Franco F. 1985 - Direct action of opioid peptides and naloxone on gonadotropin secretion by cultured rat anterior pituitary cells - Life Sci. 38: 617-625.
• Carter D.A., Baker B.I.1980 - The relationship between opiate concentration and cellular activity in the pars distalis and neurointermediate lobe of the eel pituitary - Gen. Comp. Endocrinol. 41: 225-232.
• Chao C.C., Moss G.E., Malven P.V. 1986 - Direct opioid regulation of pituitary release of bovine luteinizing hormone - Life Sci. 39(6): 527-534.
• Cheng K.W. 1996 - Molecular studies of proopiomelanocortin in golfish brain - M. Phil. Thesis. The University of Hong Kong, Hong Kong.
• Dubois M.P., Billard R., Breton B., Peter R.E. 1979 - Comparative distribution of somatostatin, LHRH, neurophysin, and α-endorphin in the rainbow trout: An immunocytochemical study - Gen. Comp. Endocrinol. 37: 220-231.
• Ferin M., Van Vugt D., Wardlow S.L. 1982 - The hypothalamic control of the menstrual cycle and the role of endogenous opioid peptides - Recent Prog. Horm. Res. 40: 441-485.
• Follénius E., Dubois M.P. 1978 - Distribution of fibres reacting with an α-endorphin antiserum in the neurohypophysis of Carassius auratus and Cyprinus carpio - Cell Tissue Res. 189: 251-256.
• Follénius E., Dubois M.P. 1979a - Différenciation immunocytologique de l'innervation hypophysaire de la Carpe a l'aide de sérums anti met-enképhaline et anti-α-endorphine - Neurobiologie Série D-47: 639-642.
• Follénius E., Dubois M.P. 1979b - Localisation des sites immunoréactifs avec un antisérum contre la met-enképhaline et contre l'α-endorphine dans le cerveau de la Carpe - Neurobiologie Série D 69: 903-906.
• Gopalan C., Currie I.S., Gilmore D.P. 1991 - Effects of opiates on hypothalamic amine turnover and LH levels in castrated rats - Biogenic Amines 8: 144-147.
• Grossman A., Moult P.J.A., Gaillard R.C., Delitala G., Toff W.D., Rees L.H., Besser G.M. 1981 - The opioid control of LH and FSH release: Effects of a met-enkephalin analogue and naloxone - Clin. Endocrinol. 14: 41-47.
• Hon W.K., Ng T.B. 1986 - Hormones with adrenocoticotropic and opiate-like activites from the carp (Cyprinus carpio) pituitary - Comp. Biochem. Physiol. C. 85(2): 443-448.
• Hunter C., Baker B.I. 1979 - The distribution of opiate activity in the trout pituitary gland - Gen. Comp. Endocrinol. 37: 111-114.
• Kah O., Pontet A., Rodriguez J.N., Calas A., Breton B. 1989 - Development of enzyme-linked immunosorbent assay for goldfish gonadotropin - Biol. Reprod. 40: 68-73.
• Kawauchi H., Tubokawa M., Kanezawa A., Kitagawa H. 1980 - Occurrence of two different endorphins in the salmon pituitary - Biochem. Biophys. Res. Commun. 92: 1278-1288.
• Leadem C.A., Kalra S.P. 1985 - Effects of endogenous opioid peptides and opiates on luteinizing hormone and prolactin secretion in ovariectomized rats - Neuroendocrinology 41: 342.
• Mikolajczyk T., Weil C., Epler P., Breton B. 1990 - Calcium ions as a mediator in GnRH action on gonadotropin release in the common carp (Cyprinus carpio L.) - Reprod. Nutr. Dev. 30: 483-492.
• Nishimura S., Pasternak G.W. 1982 - Opiate and opioid peptide binding in rat and goldfish: further evidence for opiate receptor heterogeneity - Brain Res. 248: 192-195.
• Pert C.B., Aposhian D., Snyder S.H. 1974 - Phylogenetic distribution of opiate receptor binding - Brain Res. 75:356-361.
• Pfeifer D.G., Pfeifer A., Shimohigashi Y. 1983 - Predominant involvement of mu- rather then delta- or kappa-opiate receptors in LH secretion - Peptides 4: 647-649.
• Rasmussen D.D., Gambacciani M., Swartz W. 1989 - Pulsatile gonadotropin-releasing hormone release from the human mediobasal hypothalamus in vitro: opiate receptor-mediated suppression - Neuroendocrinology 49: 150-156.
• Rosenblum P.M., Callard I.P. 1988 - The endogenous opioid peptide system in male brown bullhead catfish, Ictalurus nebulosus Lesueur: Characterization of naloxone binding and the response to naloxone during the annual reproductive cycle - J. Exp. Zool. 245: 244-255.
• Rosenblum P.M., Peter R.E. 1987 - Endogenous opioid modulation of gonadotropin secretion in male goldfish, Carassius auratus - In "Proceedings of the Third International Symposium on Reproductive Physiology of Fish." Abstract 57.
• Rosenblum P.M., Peter R.E., 1989 - The evidence for the involvement of endogenous opioids in the regulation of gonadotropin secretion in male goldfish, Carassius auratus - Gen. Comp. Endocrinol. 73: 21-27.
• Sanchez-Franco F., Cacicedo L. 1986 - Inhibitory effect of beta-endorphin on gonadotropin-releasing hormone and thyrotropin-releasing hormone releasing activity in cultured rat anterior pituitary cells - Horm. Res. 24(1): 55-61.
• Sokolowska-Mikolajczyk M., Socha M., Mikolajczyk T., Chyb J., Epler P. 2002a - The effects of naltrexone-opioid receptor antagonist on in vivo LH secretion from the pituitary of common carp (Cyprinus carpio L.) - Comp. Biochem. Physiol. 131: 417-424.
• Sokolowska-Mikolajczyk M., Socha M., Mikolajczyk T., Chyb J., Epler P. 2002b - Seasonal short term effects of naltrexone (an opioid receptor antagonist) on LH secretion in male carp (Cyprinus carpio L.) - Comp. Biochem. Physiol. 131: 379-385.
• Vallarino M. 1985 - Occurence of β-endorphin-like immunoreactivity in the brain of the teleost, Boops boops - Gen. Comp. Endocrinol. 60: 63-69.
• Veil C., Hansen P., Hyam D., Le Gac F., Breton B., Crim L.W. 1986 - Use of pituitary cells in primary culture to study regulation of gonadotropin hormone (GtH) secretion in rainbow trout: Setting up and validating the system as assessed by its responsiveness to mammalian and salmon gonadotropin releasing hormone. Gen. Comp. Endocrinol. 62: 202-209.
• Walsh J.P., Clark I.J. 1996 - Effects of central administration of highly selective opioid mu-, delta- and kappa-receptor agonists on plasma luteinizing hormone (LH), prolactin, and the estrogen-induced LH surge in ovariectomized ewes - Endocrinology 137: 3640-3648.