Regulation of ammonium and potassium uptake by glutamine and asparagine in Pisum arvense plants

• Autorzy: Kubik-Dobosz G , Buczek J.
• Języki publikacji: EN

Abstrakty

EN

Pisum arvense plants were subjected to 5 days of nitrogen deprivation. Then, in the conditions that increased or decreased the root glutamine and asparagine pools, the uptake rates of 0.5 mM NH₄⁺ and 0.5 mM K⁺ were examined. The plants supplied with 1 mM glutamine or asparagine took up ammonium and potassium at rates lower than those for the control plants. The uptake rates of NH₄⁺ and K⁺ were not affected by 1 mM glutamate. When the plants were pre-treated with 100 µM methionine sulphoximine, an inhibitor of glutamine synthesis, the efflux of NH₄⁺ from roots to ambient solution was enhanced. On the other hand, exposure of plants to methionine sulphoximine led to an increase in potassium uptake rate. The addition of asparagine, glutamine or glutamate into the incubation medium caused a decline in the rate of NH₄⁺ uptake by plasma membrane vesicles isolated from roots of Pisum arvense, whereas on addition of methionine sulphoximine increased ammonium uptake. The results indicate that both NH₄⁺ and K⁺ uptake appear to be similarly affected by glutamine and asparagine status in root cells.

Słowa kluczowe

EN

plasma membrane; cell; ammonium; glutamine; potassium; methionine sulphoximine; asparagine; root; Pisum arvense; plant

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1999
• Tom: 21
• Numer: 1
• Opis fizyczny: p.3-7,fig.

Twórcy

autor

Kubik-Dobosz G

• Wroclaw University, Kanonia 6-8, 50-328 Wroclaw, Poland

autor

Buczek J.

Bibliografia

• Bradford M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
• Deane-Drummond C. E. 1986. Some regulatory aspects of [¹⁴C] methylamine influx into Pisum sativum L. cv. Feltham First seedlings. Planta 169: 8–15.
• Feng J., Volk R. J., Jackson W. A. 1994. Inward and outward transport of ammonium in roots of maize and sorghum: contrasting effects of methionine sulphoximine. J. Exp. Bot. 45: 429–439.
• Hecht U., Mohr H., 1990. Factors controlling nitrate and ammonium accumulation in mustard (Sinapis alba) seedlings. Physiol. Plant. 78: 379–387.
• Jackson W.A., Chaillou S., Morot-Gaudry J-F., Volk R. J., 1993. Endogenous ammonium generation in maize roots and its relationship to other ammonium fluxes. J. Exp. Bot. 44: 731–739.
• Kleiner D., Castroph H. 1982. Inhibition of ammonium (methylammonium) transport in Klebsiella pneumoniae by glutamine and glutamine analogs. FEBS Lett. 146: 201–203.
• Kubik-Dobosz G., Kazanecka M., Buczek J. 1999. Regulation of NH₄⁺ transport pathways in Pisum arvense roots. Acta Physiol. Plant., 21: 9–16.
• Lee R. B., Ayling S. M. 1993. The effect of methionine sulphoximine on the absorption of ammonium by maize and barley roots over short periods. J. Exp. Bot. 44: 53–63.
• Lee R. B., Purves J. V., Ratcliffe R. G., Saker L. R. 1992. Nitrogen assimilation and the control of ammnoium and nitrate absorption by maize roots. J. Exp. Bot. 43: 1385–1396.
• Lee R. B., Rudge K. A. 1986. Effects of nitrogen deficiency on the absorption of nitrate and ammonium by barley plants. Ann. Bot. 57: 471–486.
• Maathuis F. J. M., Sanders D. 1996. Mechanism of potassium absorption by higher plant roots. Physiol. Plant. 96: 158–168.
• Marschner H. 1995. Mineral nitrition in higher plants. Academic Press, London, 889.
• Morgan M. A., Jackson W. A. 1988 a. Inward and outward movement of ammonium in root systems: transient responses during recovery from nitrogen deprivation in presence of ammonium. J. Exp. Bot. 179–191.
• Morgan M. A., Jackson W. A. 1988 b. Suppression of ammonium uptake by nitrogen supply and its relief during nitrogen limitation. Physiol. Plant. 73: 38–45.
• Rhodes D., Deal L., Haworth P., Jamieson G. C., Reuter C. C., Ericson W. 1986. Amino acid metabolism of Lemna minor L. I. Response to methionine sulfoximine. Plant Physiol. 82: 1057–1062.
• Ronzio R. A., Rowe W. B., Meister A. 1969. Studies on the mechanism of inhibition of glutamine synthetase by methionine sulfoximine. Biochem. 8: 1066–1075.
• Rufty T. W., Jackson W. A., Raper C. D. 1982. Inhibition of nitrate assimilation in roots in the presence of ammonium: the moderating influence of potassium. J. Exp. Bot. 33: 1122–1137.
• Schachtman D. P., Schroeder J. I., Lucas W. J., Anderson J. A., Gaber R. F. 1992. Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science 258: 1654–1658.
• Turpin D. H., Edie S. E., Canvin D. T. 1984. In vivo nitrogenase regulation by ammonium and methylamine and the effect of MSX on ammonium transport in Anabena flos-aquae. Plant Physiol. 74: 701–704.
• Vale F. R., Volk R. J., Jackson W. A. 1988. Simultaneous influx of ammonium and potassium into maize roots: kinetics and interactions. Planta 173: 424–431.
• Wang M. Y., Siddigi M. Y., Ruth., Glass. M. 1993. Ammonium uptake by rice roots. II. Kinetics of ¹³NH₄⁺ influx across the plasmalemma. Plant Physiol. 103: 1259–1267.
• White P. J. 1996. The permeation of ammonium through a voltage-independent K⁺ channel in the plasma membrane of rye roots. J. Membrane Biol. 152: 89–99.
• Wiame J M., Grenson M., Arst H. N. 1985. Nitrogen catabolite repression in yeast and filamentous fungi. Adv. Microb. Physiol. 26: 1–88.