Ultrastructural changes of ovarian follicle and corpus luteum after experimental zearalenone mycotoxicosis in bitch

• Autorzy: Gajecka M. , Przybylska-Gornowicz B. , Obremski K. , Polak M. , Jakimiuk E. , Skorska-Wyszynska E. , Zielonka L. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the influence of experimental zearalenone (ZEA) my- cotoxicosis on the ultrastructure of ovaries in bitches receving zearalenone for 100 days per os in the anestrus phase. Experiment has been conducted on 9 sexually mature clinically healthy bitches. The sexually mature animals aged 1-3 years were in anestrus. The dogs have been divided into three groups: EI and Ell, receiving ZEA per os in two dosages (25 μg/kg and 50 μg/kg b.w.) and control animals which received placebo per os. On the last day of experiment, ovariohysterectomy was performed in all the bitches and the uterine samples were submitted to ultrastructural analyses. The study has revealed that long lasting administration of higher dose of zearalenone causes ultrastructural changes in the granular layer of ovarian follicles, which showed due to their biological activity decrease. The changes in the granular layer, atrophy of intercellular connections and the appearance of intercellular spaces can stand for reduced activity of this layer. Changes in morphology of mitochondria, rough endoplasmic reticulum and Golgi apparatus sugest ZEA influence on cell metabolism and secretory processes. It seems therefore that in bitches and other monogastric animals, intoxications induced by this mycotoxin causes ultrastructural changes in granular cells of ovarian follicles.

Słowa kluczowe

EN

animal disease; dog; bitch; mycotoxicosis; zearalenone; ovary; ultrastructure; ovarian follicle; corpus luteum; ultrastructural change; granular cell

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2008
• Tom: 11
• Numer: 4
• Opis fizyczny: p.327-337,fig.,ref.

Twórcy

autor

Gajecka M.

• University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Przybylska-Gornowicz B.

autor

Obremski K.

autor

Polak M.

autor

Jakimiuk E.

autor

Skorska-Wyszynska E.

autor

Zielonka L.

autor

Gajecki M.

Bibliografia

• Avantaggiato G, Havenaar R, Visconti A (2003) Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointes­tinal model. Food Chem Toxicol 41: 1283-1290.
• Beijerink NJ, Buijtels JJCWM, Okkens AC, Kooistra HS, Dieleman SJ (2007) Basal and GnRH-induced secretion of FSH and LH in anestrous versus ovariectomized bitches. Theriogenology 67: 1039-1045.
• Bossy-Wetzel E, Green DR (1999) Apoptosis: checkpoints at the mitochondrial frontier. Mutat Res 434: 243-251.
• Chen J-Q, Yager JD, Russo J (2005) Regulation of mitochondrial respiratory chain structure and function by estrogens/estrogen receptors and potential physiologi- cal/pathophysiological implications. Biochim Biophys Acta 1746: 1-17.
• Conkova E, Laciakova A, Kovac G, Seidel H (2003) Fusarial toxins and their role in animal diseases. Vet J 165: 214-220.
• De Bosschere H, Ducatelle R, Tshamala M, Coryn M (2002a) Changes in sex hormone receptors during ad­ministration of progesterone to prevent estrus in the bitch. Theriogenology 58: 1209-1217.
• De Bosschere H, Ducatelle R, Vermeirsch H, Simoens P, Coryn M (2002b) Estrogen-h and progesterone receptor expression in cystic endometrial hyperplasia and py- ometra in the bitch. Anim Reprod Sci 70: 251-259.
• Diekman MA, Green ML (1992) Mycotoxins and reproduc­tion in domestic animals. J Anim Sci 70: 1615-1627.
• Gajęcka M (2006) The impact of experimental zearalenone micotoxicosis on the reproductive tract of bitches. Doc­toral thesis. University of Warmia and Mazury in Olsztyn, Poland, (in Polish).
• Gajęcka M, Jakimiuk E, Polak M, Otrocka-Domagala I, Janowski T, Zwierzchowski W, Obremski K, Zielonka Ł, Apoznański J, Gajęcki M (2004a) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
• Gajęcka M, Jakimiuk E, Skorska-Wyszyńska E, Zielonka Ł, Polak M, Paluszewski A, Rybarczyk L, Gajęcki M (2004b) Influence of zearalenone mycotoxicosis on se­lected immunological, hematological and biochemical in­dexes of blood plasma in bitches. Pol J Vet Sci 7: 177-180.
• Gajęcka M, Janowski T, Jakimiuk E, Polak M, Pod- halicz-Dzięgielewska M, Rotkiewicz T, Otrocka-Domagala I, Obremski K, Zielonka Ł, Gajęcki M (2007) Histopathological and immunohistochemical examination of the uterus in bitches following zearalenon mycotoxicosis. Pol J Vet Sci 10: 143-151.
• Grundy SA, Feldman E, Davidson A (2002) Evaluation of infertility in the bitch. Clin Tech Small Anim Pract 17: 108-115.
• Hassen W, El Goili E, Baudrimont I, Mobio AT, Ladjimim M, Creppy EE, Bacha H (2005) Cytotoxicity and Hsp 70 induction in Hep G2 cells in response to zearalenone and cytoprotcction by sub-lethal heat shock. Toxicology 207: 293-301.
• Hoffmann B, Riesenbeck A, Klein R (1996) Reproductive endocrinology of bitches. Anim Reprod Sci 42: 275-288.
• Jakimiuk E, Kuciel-Lisieska G, Zwierzchowski W, Gajęcka M, Obremski K, Zielonka Ł, Skorska-Wyszyńska E, Gajęcki M (2006) Morphometric changes of the reproductive system in gilts during zearalenone mycotoxicosis. Med Weter 62: 99-102.
• Kawano N, Koji T, Hishikawa Y, Murase K, Murata I, Kohno S (2004) Identification and localization of estro­gen receptor a- and (3- positive cells in adult male and female mouse intestine at various estrogen levels. His- tochem Cell Biol 121: 399-405.
• Lephart ED, Setchell KDR, Lund TD (2005) Phytoestro­gens: hormonal action and brain plasticity. Brain Res Bull 65: 193-198.
• Malekinejad H, Colenbrander B, Fink-Gremmels J (2006a) Hydroxysteroid dehydrogenases in bovine and porcine granulose cells convert zearalenone into its hydroxylated metabolites a-zearalenol and p-zearalenol. Vet Res Com- mun 30: 445-453.
• Malekinejad H, Van Tol HT, Collebrander B, Fink-Grem- mels J (2006b) Expression of 3a- and 3b-hydroxy steroid dehydrogenase mRNA in COCs and granulosa cells de­termines Zearalenone biotransformation. Toxicol In Vitro 20: 458-463.
• Minervini F, Giannoccaro A, Cavallini A, Visconti A. (2005) Investigations on cellular proliferation induced by zearalenone and its derivatives in relation to the es­trogenic parameters. Toxicol Lett 159: 272-283.
• Ouanes Z, Ayed-Boussema I, Baati T, Creppy EE, Bacha H (2005) Zearalenone induces chromosome aberrations in mouse bone marrow: preventive effect of 17|3-es- tradiol, progesterone and Vitamin E. Mutat Res 565: 139-149.
• Straub RH (2007) The complex role of estrogens in inflam­mation. Endocr Rev 28: 521-574.
• Sweeney T (2002) Is exposure to endocrine disrupting com­pounds during fetal/postnatal development affecting the reproductive potential of farm animals? Domest Anim Endocrin 23: 203-209.
• Turcotte JC, Hunt PJB, Blaustein JD (2005) Estrogenic ef­fects of zearalenone on the expression of progestin recep­tors and sexual behavior in female rats. Horm Behav 47: 178-184.
• Ververidis HN, Boscos CM, Stefanakis A, Saratsis P, Stamou AI, Rrambovitis E'(2004) Serum estradiol-17|3, proges­terone and respective uterine cytosol receptor concentra­tions in bitches with spontaneous pyometra. Theriogenol­ogy 62: 614-623.
• Yang J, Zhang Y, Wang Y, Cui S (2007) Toxic effects of zearalenone and alpha-zearalenol on the regulation of steroidogenesis and testosterone production in mouse Leydig cells. Toxicol In Vitro 21: 558-565.
• Zinedine A, Soriano JM, Molto JC, Manes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone. An oestrogenic mycotoxin. Food Chem Toxicol 45: 1-18.
• Zwierzchowski W, Gajgcki M, Obremski K, Zielonka L, Baranowski M (2004) The occurrence of zearalenone and its derivatives in standard and therapeutic feeds for companion animals. Pol J Vet Sci 7: 289-293.
• Zwierzchowski W, Obremski K, Zielonka L, Skorska-Wyszynska E, Gaj^cka M, Jakimiuk E, Polak M, Jana B, Rybarczyk L, Gajycki M (2006) The impact of zearalenone on the level of the selected estrogens in blond serum of sexually immature gilts. Pol J Vet Sci 9: 247-252.