A study on the toxicity of a medicinal plant, Artemisia annua L. [Asteraceae] extracts to the sunn pest, Eurygaster integriceps Puton [Hemiptera: Scutelleridae]

• Autorzy: Zibaee A , Bandani A.R.

Warianty tytułu

PL

Badanie toksycznosci wyciagow z rosliny leczniczej Artemisia annua L. w stosunku do szkodnika Eurygaster integriceps Puton [Hemiptera: Scutelleridae]

• Języki publikacji: EN

Abstrakty

EN

A methanolic extract of Artemisia annua was obtained to evaluate its insecticidal activities against the sunn pest (Eurygaster intefriceps). Also, the responses of general esterase (EST), glutathione S-transferase (GST), alkaline phosphatase (ALP), acid phosphatase (ACP), acethylcholinesterase (AChE) to the plant extract were investigated. Topical application of plant extract on adults showed that the mortality was dose-dependent i.e. with increasing of plant extract concentrations more mortality achieved. Esterase and GST activities were increased in the first 24 h post-treatment. However, the enzymes activities were decreased after 24 h until 72 h. The activities of ALP, ACP and AChE in insect body decreased significantly and inhibition was higher along with increasing concentrations of plant extract. Isozyme electrophoresis profiles indicated that responses of isozymes (EST and GST) to plant extract were decreased after 48 h exposure to extract so that some enzymes bands disappeared. The results indicated that the highest concentration of A. annua extract was the most toxic among the four extracts. The decline of the detoxification ability in insects' tissues might be the main reason for the insecticidal activities.

PL

Metanolowy wyciąg z A. annua uzyskano w celu oceny jego aktywności owadobójczej wobec szkodnika Eurygaster integriceps. Badano również reakcje ogólnej esterazy (EST), S-transferazy glutationu (GST), alkalicznej fosfatazy (ALP), kwaśnej fosfatazy (ACP) i acetylholinoesterazy (AChE) w wyciągu roślinnym. Zastosowanie wyciągu roślinnego na dorosłe osobniki wykazało, że śmiertelność zależała od dawki, to znaczy, zwiększenie stężenia wyciągu zwiększało śmiertelność. Aktywność esterazy i GST była zwiększona po 24 do 72 godzinach. Aktywność ALP, ACP i AChE w ciele owada istotnie spadała i inhibicja była wyższa w miarę zwiększania stężeń wyciągu roślinnego. Profile elektroforezy izozymów wskazywały, że reakcje izoerozymów (EST i GST) na wyciąg roślinny spadały po 48 godzinach ekspozycji na wyciąg tak, że niektóre pasma enzymów zanikły. Wyniki wskazywały, że najwyższe stężenie wyciągu A. annua było najbardziej toksyczne spośród czterech stężeń. Spadek zdolności detoksyfikacyjnej w tkankach owadów może być główną przyczyną obserwowanej aktywności owadobójczej.

Słowa kluczowe

EN

plant extract; toxicity; medicinal plant; Artemisia annua; pest; Eurygaster integriceps; Hemiptera; Scutelleridae; enzyme activity; isoenzyme

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 1
• Opis fizyczny: p.79-85,fig.,ref.

Twórcy

autor

Zibaee A

• College of Agriculture and Natural Resources, University of Tehran, Karaj 31584, Iran

autor

Bandani A.R.

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