Transformation of microspore-derivated of winter oilseed rape [Brassica napus L.] by using Agrobacterium tumefaciens

• Autorzy: Cegielska-Taras T , Pniewski T , Szala L
• Języki publikacji: EN

Abstrakty

EN

Haploid microspore-derived embryos (MDEs) constitute a unique material for the introduction of new traits into winter oilseed rape (Brassica napus). MDEs have been transformed by using Agrobacterium tumefaciens strains EHA105 and LBA4404, both carrying the binary vector pKGIB containing the uidA gene encoding -glucuronidase (GUS) and the bar gene as a marker of resistance to phosphinotricin. Transformed embryos expressed GUS and regenerated plants that were resistant to herbicide Basta, as confirmed by a leaf-painting test. Progeny plants of the transformant T-39 were all transgenic, as they inherited T-DNA from their doubled haploid parental plant. Southern-blot analysis confirmed the integration and transmission of T-DNA into T₁ plants. Transformation of MDEs facilitates the obtaining of winter oilseed rape homozygous for the introduced genes.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 4
• Opis fizyczny: p.343-347,fig.,ref.

Twórcy

autor

Cegielska-Taras T

• Plant Breeding and Acclimatization Institute, Strzeszynska 36, 60-479 Poznan, Poland

autor

Pniewski T

• Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland

autor

Szala L

• Plant Breeding and Acclimatization Institute, Strzeszynska 36, 60-479 Poznan, Poland

Bibliografia

• Cardoza V, Stewart CN, 2003. Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyls segments expiants. Plant Cell Rep 21: 599-604.
• Cegielska-Taras T, Tykarska T, Szała L, Kuraś L, Krzymański J, 2002. Direct plant development from microspore-derived embryos of winter oilseed rape Brassica napus L. ssp. oleifera (DC.) Metzger. Euphytica 124: 341-347.
• Chee PP, Slightom JL, 1994. Transformation of soybean (Glycine max) via Agrobacterium tumefaciens and analysis of transformed plants. In: Gartland KMA, Davey MR, eds. Methods in Molecular Biology, Humana Press, Totowa NJ: 101-119.
• Dorman M, Darla N, Hayden D, Puttick D, Quandt J, 1998. Non-destructive screening of haploid embryos for glufosinate ammonium resistance four weeks after microspore transformation in Brassica. Acta Hortic 459: 191-197.
• Friedt W, Zarhloul K, 2005. Haploids in the improvement of Crucifers. In: Plamer CE, Keller WA, Kasha KJ, eds. Haploids in Crop Improvement II. Springer-Verlag Berlin Heidelberg: 191-213.
• Fukuoka H, Ogawa T, Matsuoka M, Ohkawa Y, Yano H, 1998. Direct gene delivery into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants. Plant Cell Rep 17: 323-328.
• Gamborg OL, Miller RA, Ojima K, 1968. Nutrient requirements for suspension cultures of soybean root cells. Exp Cell Res 50: 151-158.
• Grison R, Grezes-Besset B, Schneider M, Lucante N, Olsen L, Leguay J-J, Toppan A, 1996. Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene. Nat Biotechnol 14: 643-646.
• Hansen G, Wright MS, 1999. Recent advances in transformation of plants. Trends Plant Sci 4: 226-231.
• Huang B, 1992. Genetic manipulation of microspores and microspore-derived embryos. In Vitro Cell Dev Biol 28P: 53-58.
• Jefferson RA, 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387-405.
• McGarvey P, Kaper JM, 1991. A simple and rapid method for screening transgenic plants using the PCR. BioFeedback 4: 428-432.
• Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473-479.
• Nałęczyńska A, Cegielska T, 1984. Dihaploid production in Brassica napus L. by in vitro androgenesis. Genet Pol 25:271-276.
• Oelck MM, Phan CV, Eckes P, Donn G, Rakow G, Keller WA, 1991. Field resistance of canola transformants (Brassica napus L.) to Ignite™ (phosphinotricin). Proc. 8th Int. Rapeseed Congress, 292-297.
• Pechan PM, 1989. Successful cocultivation of Brassica napus microspores and proembryos with Agrobacterium. Plant Cell Rep 8: 387-390.
• Pniewski T, Kapusta J, 2005. Efficiency of transformation of Polish cultivars of pea (Pisum sativum L.) with various regeneration capacity by using hypervirulent Agrobacterium tumefaciens strains. J Appl Genet 46: 139-147.
• Poulsen GB, 1996. Genetic transformation of Brassica. Plant Breeding 115: 209-225.
• Takahata Y, Fukuoka H, Wakui K, 2005. Utilization of microspore-derived embryos. In: Plamer CE, Keller WA, Kasha KJ, eds. Haploids in Crop Improvement II, Springer-Verlag Berlin Heidelberg: 153-169.
• Troczyńska J, Drozdowska L, Cegielska-Taras T, 2003. Transformation of microspore-derived embryos to study the myrosinase-glucosinolate system in Brassica napus L. Proc. 11th Int. Rapeseed Congress, 175-177.
• Tsukazaki H, Kuginuki Y, Aida R, Suzuki T, 2002. Agrobacterium-meditted transformation of a doubled haploid line of cabbage. Plant Cell Rep 21: 257-262.
• Vancanneyt G, Schmidt R, O’Connor-Sanchez A, Willmitzer L, Rocha-Sosa M, 1990. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245-250.
• Wang J, Chen Z, Du J, Sun Y, Liang A, 2005. Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes. Plant Cell Rep 24: 549-555.
• Wang YP, Sonntag K, Rudloff E, Han J, 2005. Production of fertile transgenic Brassica napus by Agrobacterium-mediated transformation of protoplasts. Plant Breeding 124: 1-4.
• Weber S, Ünker F, Friedt W, 2005. Improved doubled haploid production protocol for Brassica napus using microspore colchicine treatment in vitro and ploidy determination by flow cytometry. Plant Breeding 124: 511-513.
• Westecott MB, Huang B, 1995. Application of haploidy in genetic manipulation of canola. In: Terzi M et al., eds. Current Issues in Plant Molecular and Cellular Biology, Kluwer Academic Publisher: 143-148.
• Zhang FL, Takahata Y, Watanabe M, Xu JB, 2000. Agrobacterium-mediated transformation of cotyledonary expiants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 19: 569-575.
• Zhong GY, 2001. Genetic issues and pitfalls in transgenic plant breeding. Euphytica 118: 137-144.