Expression of human serum albumin - L7-L12 [Brucella abortus ribosomal protein] fusion protein in Saccharomyces cerevisiae

• Autorzy: Pakzad I , Rezaee A. , Emaneini M. , Hosseini A.Z. , Tabbaraee B. , Taherikalani M.
• Języki publikacji: EN

Abstrakty

EN

Brucella abortus is a facultative intracellular gram-negative bacterial pathogen that causes abortion in pregnant cattle and undulant fever in humans. The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. It has already been expressed in several bacteria and has been used as DNA vaccine. In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the 171112 ribosomal gene was amplified by PCR. The expression plasmid pYtHSA-L7/Ll 2 was constructed by inserting the L7/L12 gene into the pYHSA5 shuttle vector (containing inulinase signal sequence, HSA gene and Gal 10 promoter). The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L 12 antibodies. Fusion protein was purified by affinity chromatography and its amount was approximately 500 μg/liter.

Słowa kluczowe

EN

serum albumin; man; gene expression; bacteria; Brucella abortus; human disease; brucellosis; zoonosis; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 2
• Opis fizyczny: p.99-104,fig.,ref.

Twórcy

autor

Pakzad I

• Ilam University of Medical Sciences, Ilam, Iran

autor

Rezaee A.

autor

Emaneini M.

autor

Hosseini A.Z.

autor

Tabbaraee B.

autor

Taherikalani M.

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