Immunohistochemical identification method of tumour cells in the S phase of mitotic cycle and its usefulness in diagnostics of mammary gland adenocarcinomas in bitches

• Autorzy: Nowak M. , Madej J.A. , Dziegiel P. , Kanzawa H.
• Języki publikacji: EN

Abstrakty

EN

The studies aimed at identification of neoplastic cells at the S phase of mitotic cycle in mammary gland adenocarcinomas of bitches. The material was sampled from bitches of various races, aging 6 to 12 years, in which the mammary gland tumours developed spontaneously. The tumours were verified histopathologically and, then, immunohistochemical reactions were performed in order to detect cells which had incorporated BrdU (bromodeoxyuridine), contained Ki-67 or PCNA antigen. The histological preparations were photographed and obtained pictures were subjected to computer-assisted image analysis using Axiophot microscope (Carl Zeiss) coupled to a computer and the MultiScaneBase V 8.08 software, working under Windows. Fifty percent of sections from mammary gland adenocarcinomas demonstrated BrdU labelling index of 4-5%, 40% of 1-3%, while in the remaining 10% of examined tumours no BrdU incorporation could be demonstrated. No evident relationship could be detected between the presence of BrdU incorporation and Ki-67 or PCNA antigen presence but a significant correlation was demonstrated between the expression of Ki-67 and PCNA.

Słowa kluczowe

EN

tumour cell; bitch; veterinary medicine; neoplastic cell; monoclonal antibody; mitotic cycle; adenocarcinoma; immunohistochemistry; mammary gland carcinoma; mammary gland; diagnostics; identification

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2006
• Tom: 09
• Numer: 1
• Opis fizyczny: p.57-62,fig.,ref.

Twórcy

autor

Nowak M.

• Agricultural Academy, Norwida 31, 50-375 Wroclaw, Poland

autor

Madej J.A.

autor

Dziegiel P.

autor

Kanzawa H.

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