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2004 | 53 | 1 |

Tytuł artykułu

Antigenicity of the Campylobacter coli CjaA protein produced by Escherichia coli

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Immunogenic C. coli CjaA protein is a candidate for a chicken anti-Campylobacter subunit vaccine. In order to enhance its immunogenicity an antigenic determinant of the CjaA protein was identified. Thereafter, two copies of the antigenic epitope were cloned in tandem directly or with a flexible hinge between them. All experiments documented that the CjaA protein contained not only linear antigenic epitope/s but also conformational ones.

Wydawca

-

Rocznik

Tom

53

Numer

1

Opis fizyczny

p.61-64,fig.,ref.

Twórcy

autor
  • Warsaw University, Miecznikowa 1 str., 02-096 Warsaw, Poland
autor

Bibliografia

  • Boos W.F. and J.M. Lucht. 1994. Periplasmic binding protein dependent ABC transports, p. 1175-1210. In: R. Curtiss III, J.L. Ingraham, E.C.C. Lin, K. Brooks Low, B. Magasanik, W.S. Reznikoff, M. Schaechter and U.H.E. (eds), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. ASM, Washington, D.C.
  • Brinkmann U., R.E. Mattes and P. Buckel. 1989. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene 85: 109-114.
  • Chatfield S.N. and G. Dougan. 1997. Attenuated Salmonella as a live vector for expression of foreign antigens, p. 331-362. In: M.N. Levine, G.C. Woodrow, J.B. Kaper and G.S. Cobon (eds), New Generation Vaccine. Marcel Dekker, Inc, New York.
  • Dietrich G., S. Spreng, D. Favre, J.K. Viret and C.A. Guzman. 2003. Live attenuated bacteria as vectors to deliver plasmid DNA vaccines. Curr. Opin. Mol. Ther. 5: 10-19.
  • Jagusztyn-Krynicka E.K., J.E. Clark-Curtiss and R. Curtiss. 1993. Escherichia coli heat-labile toxin subunit B fusions with Streptococcus sobrinus antigens expressed by Salmonella typhimurium oral vaccine strains: importance of the linker for antigenicity and biological activities of the hybrid proteins. Infect. Immun. 61: 1004-1015.
  • Kane J.F. 1995. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherischia coli. Curr. Biotechnol. 6: 494-500.
  • Ketley J.M. 1997. Pathogenesis of enteric infection by Campylobacter. Microbiology 143: 5-21.
  • Lis M., A. Wyszyńska, A. Raczko, R. Godlewska and E.K. Jagusztyn-Krynicka. 2003. Bivalent anti-Salmonella/Campylobacter chicken vaccine - effect of oral immunization with cocktail vaccine prototype. In: XIIth International Workshop on Campylobacter, Helicobacter and related microrganisms, Aarhus, Danmark, 2003.
  • Nachampkin I., K. Bohachick and C. Pallon. 1993. Flagellin genotyping of Campylobacter jejuni by restriction fragments length polimorphism RFLP analysis. J. Clin. Microbiol. 31: 1531-1536.
  • Pawelec D.P., D. Korsak, A.K. Wyszyńska, E. Rożynek, J. Popowski and E.K. Jagusztyn-Krynicka. 2000. Genetic diversity of the Campylobacter genes coding immunodominant proteins. FEMS Microbiol Lett. 185: 43-49.
  • Redman T.K., C.C. Harmon and S.M. Michalek. 1996. Oral immunization with recombinant Salmonella typhimurium expressing surface protein antigen A (SpaA) of Streptococcus sobrinus: effects of the Salmonella virulence plasmid on the induction of protective and sustained humoral responses in rats. Vaccine 14: 868-878.
  • Schwerer B. 2002. Antibodies against gangliosides: a link between preceding infection and immunopathogenesis of Guillain-Barré syndrome. Microbes Infect. 4: 373-384.
  • Spratt B.G., P.J. Hedge, S. Heesen, A. Edelman and J.K. Broome-Smith. 1986. Kanamycin-resistant vectors that are analogues of plasmid pUC8, pUC9, pEMBL8. Gene 41: 337-342.
  • Zhang Q., Y.Q. Yang, Z.Y. Zhang, L. Li, W.Y. Yan, W.J. Jiang, A.G. Xin, C.X. Lei and Z.X. Zheng. 2002. Immunogenicity of a recombinant fusion protein of tandem repeat epitopes of foot-and-mouth disease virus type Asia 1 for guinea pigs. Acta Virol. 46: 1-9.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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