Gene expression profile analysis in laryngeal cancer by high-density oligonucleotide microanalysis

• Autorzy: Markowski J. , Oczko-Wojciechowska M. , Gierek T. , Jarzab M. , Paluch J. , Kowalska M. , Wygoda Z. , Pfeifer A. , Tyszkiewicz T. , Jarzab B. , Niedzielska I. , Borgiel-Marek H.
• Języki publikacji: EN

Abstrakty

EN

The assessment of gene expression profile in laryngeal cancer shall allow to implement molecular biology methods in diagnostics, as well as in prognosis of the course of disease. Thus, it may influence the choice of the most optimal decisions in regards to the method of treatment, extent of surgical procedure, or the necessity of adding post-operative radiotherapy. The aim of the project was to analyse the gene expression profile of laryngeal cancer using oligonucleotide microarrays, aiming to derive novel molecular markers for that carcinoma. The study comprised a group of 14 patients (12 males and 2 females) with squamous cell laryngeal carcinoma, diagnosed and surgically treated between 2005 – 2007 in the ENT Department of the Silesian Medical University in Katowice, Poland. RNA was isolated from frozen tissue fragments. To assess gene expression profile, high density oligonucleotide microarrays (Affymetrix U 133 Plus 2.0) were applied, with over 54 thousand probesets for over 47 thousand transcripts. Four genes, previously not assesed in diagnostic context in laryngeal carcinoma, seemed to be valuable markers of that neoplasm. These are: metalloproteinase ADAM12, cycline-dependent kinase 2 - CDK2, kinesine 14 - KIF14, suppressor 1 of checkpoint - CHES1.

Słowa kluczowe

EN

gene expression; high-density oligonucleotide microarray; metalloproteinase; post-operative radiotherapy; diagnostics; laryngeal cancer; molecular biology; human disease; oligonucleotide microarray

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2009
• Tom: 60
• Numer: 1
• Opis fizyczny: p.57-63,fig.,ref.

Twórcy

autor

Markowski J.

• Silesian Medical University, 20-24 Francuska Street, Katowice, Poland

autor

Oczko-Wojciechowska M.

autor

Gierek T.

autor

Jarzab M.

autor

Paluch J.

autor

Kowalska M.

autor

Wygoda Z.

autor

Pfeifer A.

autor

Tyszkiewicz T.

autor

Jarzab B.

autor

Niedzielska I.

autor

Borgiel-Marek H.