Influence of combined fungicides and adjuvants application on enzymatic activity and ATP content in soil

• Autorzy: Klodka D. , Nowak J.
• Języki publikacji: EN

Abstrakty

EN

In laboratory experiment the influence of different dosages of fungicides on the activity of acid and alkaline phosphatase, dehydrogenase, urease, b-glucosidase and ATP content in loamy clay soil was determined. The fungicides were applied alone and with additions of an adiuvants applied at field rate and 10-fold and 100-fold higher. The following combinations were used: Miedzian 50 WP (a.i. copper oxychloride), Miedzian 350 extra SC (Miedzian 50 WP + Polybutene emulsion) and Miedzian 50 WP applied together with Rapol 90 EC. The soil sample was submitted to analysis in 1, 3, 7, 14, 28, 56 and 112 days after the pesticides treatment. The investigations showed that soil enzymes activity after use pesticides with adiuvants was lower from activities these enzymes in soil after use of fungicides without adiuvants, it was especially visible in case of dehydrogenase and b-glucosidase.

Słowa kluczowe

EN

adenosine triphosphate; fungicide; acid phosphatase; combined fungicide; beta-glucosidase; soil; adjuvant application; alkaline phosphatase; enzyme activity; dehydrogenase; laboratory experiment; urease

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Environmental Development
• Rocznik: 2004
• Tom: 07
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/series/volume7/issue1/environment/art-01.html

Twórcy

autor

Klodka D.

• Agricultural University of Szczecin, Slowackiego 17, 71-434 Szczecin, Poland

autor

Nowak J.

Bibliografia

• Balicka N., 1983. Różne formy wzajemnego oddziaływania drobnoustrojów z herbicydami [Various forms of interrelationship between microorganisms and herbicides]. Postępy Mikrobiol. XXII, 3/4, 291-299 [in Polish].
• Bonmati M., Coccanti B., Nannipieri P., 1991. Spatial variability of phosphatase, urease, protease, organic carbon and total nitrogen in soil. Soil Biol. Biochem. 4, 391-396.
• Eivazi F., Tabatabai M.A., 1977. Phosphatases in soil. Soil Biol. Biochem. 9, 167-192.
• Hoffman G., Dedecen M., 1965. Eine Methode zur colorimetrischen Bestimmung der b-glucosidase – Aktivität im Boden [Method of colorimetric assay of b-glucosidase activity in soil]. Zeitschrift für Pflanzenernähg, Düngung bodenkunde. 108 Band, Heft. 3, 193-198 [in German].
• Ismail B.S., Yapp K.F., Omar O., 1998. Effects of metsulfuron-methyl on amylase, urease, and protease activities in two soils. Australian Journal of Soil Research 36, 3, 449-456.
• Kanazawa S., Filip Z., 1986. Distribution of microorganisms, total biomass, and enzyme activities in different particles of brown soil. Microb. Ecol. 12, 205-215.
• Kucharski J., Jastrzębska E., Wyszkowska J., Hłasko A., 2000. Wpływ zanieczyszczenia gleby olejem napędowym i benzyną ołowiową na jej aktywność enzymatyczną [Effect of pollution with diesel oil and leaded petrol on enzymatic activity of the soil]. Zesz. Probl. Postęp. Nauk. Rol. 472, 457-464 [in Polish].
• Malkomes H.-P., 1989. Einfluß der Lagerung von Bodenproben auf den Nachweis von Herbizid-Effekten auf mikrobielle Aktivität [Identification of herbicide effectson microbial activities in soil samples after storage]. Zentralbl. Mikrobiol. 144, 389-398 [in German].
• Malkomes H.-P., 1993. Eine modifizierte Methode zur Erfassung der Dehydrogenase – Aktivität (TTC – reduction) im Boden nach Herbizidenwendung [A modified method to examine the dehydrogenase activity (TTC reduction) in soil after herbicide treatments]. Nachrichtenbl. Deut. Pflanzenschultzd. 45(9), 180 [in German].
• Malkomes H.-P., 1997. Applications of ecotoxicity tests to assess side effects of pesticides in soils. Soil Ecotoxicology. CRC Press, 319-343.
• Margesin R., 1996. Acid and alkaline Phosphomooesterase Activity with the Substrate p-Nitrophenyl Phosphate. In: Method in soil Biology. Eds. F. Schinner, R. Öhlinger, E. Kandeler, R. Margesin, Springer, Berlin, 213-217.
• Margesin R., 1996. ATP by Sulfuric Acid Extraction Technique. In: Method in soil Biology. Eds. F. Schinner, R. Öhlinger, E. Kandeler, R. Margesin, Springer, Berlin, 260-265.
• Megharaj M., Boul H.L., Thiele J.H., 1999. Effects of DDT and its metabolites on soil algae and enzymatic activity. Biol Fertil Soils 29, 130-134.
• Nowak J., Nowak A., Lech B., Turos-Biernacka M., 1998. Auswirkung von Betanal 160 EC in kombination mit Zusatzstoffen auf die biologische aktivität des Boden. Teil. II. Einfluß auf die Aktivität von Bodenenzymen [Effect of Betanal EC 160 in combination with adjuvants on the biological activity of the soil. Part II: Effect on soil enzyme activity]. PflKrankh. PflSchutz. XVI, 771-778 [in German].
• Nowak J., Nowak A., Kłódka D., Turos-Biernacka M., 2000. Einfluss der gemeinsamen und getrennten Applikation von Herbiziden und Zusatzstoffen auf die Aktivität von Dehydrogenase und Phosphatase im Boden [Influence of different herbicide dosages on the phosphatase and dehydrogenase activity in soil]. PflKrankh. PflSchutz. XVII, 769-774 [in German].
• Paryluk D., 1997. Możliwości ograniczenia zużycia pestycydów w rolnictwie [Possibilities of limitation use of pesticides in agriculture]. Aura 1, 13-15 [in Polish].
• Perucci P., Scarponi L.,1994. Effects of the herbicide imazethapyr on soil microbial biomass and various soil enzyme activities. Biol Fertil Soils 17, 237-240.
• Ptaszkowska J., Bakuniak E., Maliński Z.T., 1996. Podsumowanie badań łanowych z zastosowaniem Siarkolu K 1000 SC i zaprawy nasiennej Funaben T 450 FS w ochronie zbóż [Summary of field reaserches with Siarkol K 1000 SC and seed dressing Funaben T 450 FS used in cereals protection]. Postępy Ochr. Rośl. 36(2), 247-249 [in Polish].
• Rola J., Rola H., 1998. Efektywność biologiczno-ekonomiczna “wspomagaczy” a stosowanie herbicydów [Biological-economical efficiency of adjuvants versus herbicides applying]. Mat. XXVIII Sesji Nauk. IOR, Cz. II – Postery, Poznań, 364-367 [in Polish].
• Shukla A.K., 1997 Effect of herbicides butachlor, fluchloralin, 2,4-D and oxyfluorfen on microbial population and enzyme activities of rice field soil. Indian Journal of Ecology 24, 2, 189-192.
• Sobótka W., 1999. Herbicydy – wczoraj i dziś [Herbicides – yesterday and today]. Postępy Ochr. Rośl. 39(1), 218-223 [in Polish].
• Tabatabai M.A., Bremner J.M., 1969. Use of nitrophenylophosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 19, 281-287.
• Tu C.M., 1992. Effect of some herbicides on activities of microogranisms and enzymes in soil. J. Environ. Sci. Health B27(6), 695-709.
• Węgorek W., 1994. Badania wpływu pestycydów na środowisko rolnicze [Investigations of influence of pesticides on agricultural environment]. Postępy Nauk. Rol. 2, 59-63 [in Polish].